METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS

    公开(公告)号:US20180348230A1

    公开(公告)日:2018-12-06

    申请号:US16001613

    申请日:2018-06-06

    摘要: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.