公开(公告)号：US20060110741A1

公开(公告)日：2006-05-25

申请号：US10540897

申请日：2003-12-25

申请人： Ryoichi Asai ,  Katsutoshi Takahashi ,  Ali Arjomand

发明人： Ryoichi Asai ,  Katsutoshi Takahashi ,  Ali Arjomand

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06 ,  C12Q2565/501 ,  C12Q2563/107 ,  C12Q2523/125

摘要： There is disclosed a process for detection of DNA methylation at CpG sites using nucleic acid arrays and preferably microarrays. Specifically, there is disclosed a process for directly generating a reference sample from the sample to be tested and detecting methylation at large numbers of CpG island sites simultaneously. More specifically, the inventive process comprises dividing a DNA sample into two samples (a first sample and a second sample), amplifying the first DNA sample by a nucleic acid amplification process such that any methylcytosine residues are amplified as unmethylated cytosine residues, treating the amplified first sample and the (unamplified) second sample with bisulfite to convert unmethylated cytosine residues in both samples to deoxyuracil residues, labeling the bisulfite-converted second sample with a second fluorescent marker and the bisulfite-converted first sample with a first fluorescent marker, wherein the first and second fluorescent markers have non-overlapping fluorescent excitation and emission spectra; and hybridizing the first sample and the second sample onto a microarray device having a plurality of oligonucleotide capture probes designed to hybridize to CpG island sites of the DNA sample as converted and non-converted by bisulfite.

摘要翻译： 公开了使用核酸阵列，优选微阵列检测CpG位点处DNA甲基化的方法。 具体地，公开了从待测样品直接生成参考样品并同时检测大量CpG岛位点甲基化的方法。 更具体地，本发明的方法包括将DNA样品分成两个样品（第一样品和第二样品），通过核酸扩增方法扩增第一DNA样品，使得任何甲基胞嘧啶残基被扩增为未甲基化的胞嘧啶残基， 第一样品和具有亚硫酸氢盐的（未扩增的）第二样品，以将两个样品中的未甲基化的胞嘧啶残基转化为脱氧尿嘧啶残基，用第二荧光标记物标记亚硫酸氢盐转化的第二样品，并用第一荧光标记物将亚硫酸氢盐转化的第一样品标记，其中 第一和第二荧光标记具有非重叠的荧光激发和发射光谱; 并将第一样品和第二样品杂交到具有多个寡核苷酸捕获探针的微阵列装置上，所述寡核苷酸捕获探针被设计为与DNA样品的CpG岛位点杂交，由亚硫酸氢盐转化而不转化。

公开(公告)号：US20120115238A1

公开(公告)日：2012-05-10

申请号：US13381817

申请日：2010-05-20

申请人： Shuichi Akashi ,  Ryoichi Asai

发明人： Shuichi Akashi ,  Ryoichi Asai

IPC分类号： G01N33/50 ,  B01L3/00

CPC分类号： B01L3/502 ,  B01L3/5085 ,  B01L3/527 ,  B01L2300/0681 ,  G01N2035/0436 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A nucleic acid extraction kit, which enables the nucleic acid extraction operation to be accomplished safely without causing contamination, and in which the complex preparation of reagents and the disposal treatments that are performed before and after the nucleic acid extraction operation can be performed rapidly and simply, with the extraction performed in an automated manner. The nucleic acid extraction kit includes: a container including reagent wells 2 to 9 that each store at least a reagent, a sample well 13 into which a biological sample is introduced, a waste liquid well, and a collection well 11 in which an extracted nucleic acid is collected, and an extraction filter cartridge 12 equipped with an extraction filter for separating and extracting a nucleic acid from the biological sample, wherein the extraction filter cartridge is formed in a manner that enables the extraction filter cartridge to be supported on the waste liquid well and the collection well.

摘要翻译： 一种核酸提取试剂盒，其能够安全地实现核酸提取操作而不引起污染，并且其中可以快速且简单地进行在核酸提取操作之前和之后进行的试剂和处理处理的复杂制备 ，以自动方式进行提取。 核酸提取试剂盒包括：包含试剂孔2至9的容器，其各自存储至少一种试剂，其中引入生物样品的样品孔13，废液井以及收集井11，其中提取的核酸 收集酸，以及提取滤筒12，其配备有用于从生物样品中分离和提取核酸的提取过滤器，其中提取过滤器滤芯的形成方式为使得提取滤筒能够被支撑在废液上 好和收集好。

公开(公告)号：US20070207457A1

公开(公告)日：2007-09-06

申请号：US10551156

申请日：2004-03-24

申请人： Ryoichi Asai ,  Shinichiro Nishimura ,  Katsutoshi Takahashi

发明人： Ryoichi Asai ,  Shinichiro Nishimura ,  Katsutoshi Takahashi

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： G01N33/543 ,  C12Q1/6837 ,  G01N33/5308 ,  C12Q2525/205

摘要： A microarray originally developed for analyzing intracellular RNA expressions can be used to easily load polynucleotides onto a chip and to instantaneously determine polynucleotides (aptamers) capable of binding to a target molecule. Namely, the present inventors discovered that a microarray can be used to rapidly and efficiently obtain aptamers that bind to a target molecule.

摘要翻译： 最初用于分析细胞内RNA表达的微阵列可用于容易地将多核苷酸加载到芯片上并且瞬时确定能够结合靶分子的多核苷酸（适体）。 也就是说，本发明人发现微阵列可用于快速有效地获得与目标分子结合的适体。

公开(公告)号：US08404489B2

公开(公告)日：2013-03-26

申请号：US13381817

申请日：2010-05-20

申请人： Shuichi Akashi ,  Ryoichi Asai

发明人： Shuichi Akashi ,  Ryoichi Asai

IPC分类号： G01N33/50 ,  G01N1/18 ,  B01L3/00

CPC分类号： B01L3/502 ,  B01L3/5085 ,  B01L3/527 ,  B01L2300/0681 ,  G01N2035/0436 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A nucleic acid extraction kit, which enables the nucleic acid extraction operation to be accomplished safely without causing contamination, and in which the complex preparation of reagents and the disposal treatments that are performed before and after the nucleic acid extraction operation can be performed rapidly and simply, with the extraction performed in an automated manner. The nucleic acid extraction kit includes: a container including reagent wells that each store at least a reagent, a sample well into which a biological sample is introduced, a waste liquid well, and a collection well in which an extracted nucleic acid is collected, and an extraction filter cartridge equipped with an extraction filter for separating and extracting a nucleic acid from the biological sample, wherein the extraction filter cartridge is formed in a manner that enables the extraction filter cartridge to be supported on the waste liquid well and the collection well.

摘要翻译： 一种核酸提取试剂盒，其能够安全地实现核酸提取操作而不引起污染，并且其中可以快速且简单地进行在核酸提取操作之前和之后进行的试剂和处理处理的复杂制备 ，以自动方式进行提取。 核酸提取试剂盒包括：容器，其包含各自存储至少一种试剂的试剂孔，其中引入生物样品的样品井，废液阱以及其中收集提取的核酸的收集阱，以及 提取滤筒，其具有用于从所述生物样品中分离和提取核酸的提取过滤器，其中所述提取过滤器滤芯的形成方式使得所述提取滤筒能够充分地被支撑在所述废液和所述收集井上。