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公开(公告)号:US20080248524A1
公开(公告)日:2008-10-09
申请号:US11587947
申请日:2005-04-26
摘要: It is intended to provide a thermostable AMP deaminase originating in a microorganism. Namely, an AMP deaminase having the following characteristics. (1) Catalyzing the reaction: 5′-adenylic acid+H2O→5′-inosinic acid+NH3; (2) being stable at a temperature of 65° C. or below (in an acetate buffer (pH 5.6)); (3) having a molecular weight of 48,000±2,000 in gel filtration; and (4) having the optimum pH value at around 5.6 (in McIlvaine buffer).
摘要翻译: 旨在提供源自微生物的热稳定AMP脱氨酶。 即,具有以下特征的AMP脱氨酶。 (1)催化反应:5'-腺苷酸+ H 2 O 5→5'-肌苷酸+ NH 3 (2)在65℃或更低的温度(在乙酸盐缓冲液(pH5.6))中稳定); (3)在凝胶过滤中分子量为48,000±2,000; 和(4)具有约5.6的最佳pH值(麦克林蛋白缓冲液)。
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公开(公告)号:US07709240B2
公开(公告)日:2010-05-04
申请号:US11587947
申请日:2005-04-26
摘要: It is intended to provide a thermostable AMP deaminase originating in a microorganism. Namely, an AMP deaminase having the following characteristics. (1) Catalyzing the reaction: 5′-adenylic acid+H2O→5′-inosinic acid+NH3; (2) being stable at a temperature of 65° C. or below (in an acetate buffer (pH 5.6)); (3) having a molecular weight of 48,000±2,000 in gel filtration; and (4) having the optimum pH value at around 5.6 (in McIlvaine buffer).
摘要翻译: 旨在提供源自微生物的热稳定AMP脱氨酶。 即,具有以下特征的AMP脱氨酶。 (1)催化反应:5'-腺苷酸+ H 2 O→5'-肌苷酸+ NH 3; (2)在65℃或更低的温度(在乙酸盐缓冲液(pH5.6))中稳定); (3)在凝胶过滤中分子量为48,000±2,000; 和(4)具有约5.6的最佳pH值(麦克林蛋白缓冲液)。
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