公开(公告)号：US08883495B2

公开(公告)日：2014-11-11

申请号：US12282977

申请日：2007-03-15

申请人： Shuji Nakamura ,  Ryuichi Motoda ,  Masayoshi Kibata ,  Kunzo Orita ,  Fumiyuki Yamasaki

发明人： Shuji Nakamura ,  Ryuichi Motoda ,  Masayoshi Kibata ,  Kunzo Orita ,  Fumiyuki Yamasaki

IPC分类号： C12N5/00 ,  A61K35/14 ,  C12N5/0783 ,  A61K35/12

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K2035/122 ,  A61K2300/00

摘要： The present invention has objects to provide a novel human T-cell population having both cytotoxic and immunosuppressive activities, and to a method for preparing the same. The above objects are attained by providing a human T-cell population which is obtainable by coculturing mononuclear cells, collected from human blood, with stroma cells and which has the following features: (1) being positive for CD3, CD25, CD28 and T-cell antigen receptor αβ; (2) essentially consisting of three groups of a CD4 positive and CD8 positive (CD4+CD8+) T-cell group, a CD4 positive and CD8 dimly positive (CD4+CD8dim) T-cell group, and a CD4 negative and CD8 positive (CD4−CD8+) T-cell group; (3) exerting a cytotoxic activity against the cocultured stroma cells; and (4) exerting an immunosuppressive activity against activated T cells.

摘要翻译： 本发明的目的是提供具有细胞毒性和免疫抑制活性两者的新型人T细胞群及其制备方法。 上述目的是通过提供通过共培养从人血液收集的单核细胞与基质细胞获得的人类T细胞群而获得的，并且具有以下特征：（1）对于CD3，CD25，CD28和T- 细胞抗原受体α＆bgr; （2）基本上由CD4阳性和CD8阳性（CD4 + CD8 +）T细胞组，CD4阳性和CD8阳性（CD4 + CD8dim）T细胞组和CD4阴性和CD8阳性（CD4 + CD4-CD8 +）T细胞组; （3）对共培养的基质细胞发挥细胞毒活性; 和（4）对活化的T细胞施加免疫抑制活性。

公开(公告)号：US5126148A

公开(公告)日：1992-06-30

申请号：US526143

申请日：1990-05-22

申请人： Masashi Kurimoto ,  Ryuichi Motoda ,  Kanso Iwaki

发明人： Masashi Kurimoto ,  Ryuichi Motoda ,  Kanso Iwaki

IPC分类号： C12P21/00 ,  A61K31/00 ,  A61K35/14 ,  A61K38/00 ,  A61P35/00 ,  C07K14/005 ,  C07K14/195 ,  C07K14/47 ,  C07K14/52 ,  C12N5/02 ,  C12R1/91 ,  G01N33/574

CPC分类号： C07K14/52 ,  C07K14/4703 ,  A61K38/00

摘要： Human hematopoietic cells produce metastasis-inhibitory factor (MIF). MIF exhibits a remarkable metastasis-inhibitory activity on viral diseases and immunopathies, as well as on malignant tumors. The MIF-producing human hematopoietic cells are easily proliferative by in vitro tissue culture and in vivo proliferation using a non-human warm-blooded animal. T cells exhibit a high MIF producibility. Mitogens augment the production of MIF when used as an MIF inducer.

公开(公告)号：US20090297490A1

公开(公告)日：2009-12-03

申请号：US12282977

申请日：2007-03-15

申请人： Shuji Nakamura ,  Ryuichi Motoda ,  Masayoshi Kibata ,  Kunzo Orita ,  Fumiyuki Yamasaki

发明人： Shuji Nakamura ,  Ryuichi Motoda ,  Masayoshi Kibata ,  Kunzo Orita ,  Fumiyuki Yamasaki

IPC分类号： A61K35/12 ,  C12N5/08

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K2035/122 ,  A61K2300/00

摘要： The present invention has objects to provide a novel human T-cell population having both cytotoxic and immunosuppressive activities, and to a method for preparing the same. The above objects are attained by providing a human T-cell population which is obtainable by coculturing mononuclear cells, collected from human blood, with stroma cells and which has the following features: (1) being positive for CD3, CD25, CD28 and T-cell antigen receptor αβ; (2) essentially consisting of three groups of a CD4 positive and CD8 positive (CD4+CD8−) T-cell group, a CD4 positive and CD8 dimly positive (CD4+CD8dim) T-cell group, and a CD4 negative and CD8 positive (CD4−CD8+) T-cell group; (3) exerting a cytotoxic activity against the cocultured stroma cells; and (4) exerting an immunosuppressive activity against activated T cells.

摘要翻译： 本发明的目的是提供具有细胞毒性和免疫抑制活性两者的新型人T细胞群及其制备方法。 上述目的是通过提供通过共培养从人血液收集的单核细胞与基质细胞获得的人类T细胞群而获得的，并且具有以下特征：（1）对于CD3，CD25，CD28和T- 细胞抗原受体 （2）基本上由CD4阳性和CD8阳性（CD4 + CD8-）T细胞组，CD4阳性和CD8阳性（CD4 + CD8dim）T细胞组和CD4阴性和CD8阳性三组组成 （CD4-CD8 +）T细胞组; （3）对共培养的基质细胞发挥细胞毒活性; 和（4）对活化的T细胞施加免疫抑制活性。