公开(公告)号：US5496937A

公开(公告)日：1996-03-05

申请号：US239760

申请日：1994-05-09

申请人： Yoshio Okamoto ,  Naoki Enomoto ,  Sachiko Furukawa ,  Yasushi Ogasawara ,  Hirofumi Akano ,  Yoshiya Kawamura

发明人： Yoshio Okamoto ,  Naoki Enomoto ,  Sachiko Furukawa ,  Yasushi Ogasawara ,  Hirofumi Akano ,  Yoshiya Kawamura

IPC分类号： B01J20/32 ,  C07B57/00 ,  C08B37/00 ,  B01D15/08 ,  C08B33/02 ,  G01N30/48

CPC分类号： B01J20/3274 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/29 ,  B01J20/3092 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3227 ,  B01J20/3246 ,  B01J20/3257 ,  B01J20/3259 ,  B01J20/3261 ,  B01J20/3263 ,  C07B57/00 ,  C08B37/00 ,  B01J2220/4825 ,  B01J2220/54 ,  B01J2220/58

摘要： A polysaccharide substance having the following formula is provided: ##STR1## Also provided is a method of producing the polysaccharide substance in which an oligosaccharide having a degree of polymerization from 3 to 10 is chemically bonded to a silane agent at the reducing terminal of the resultant oligosaccharide, the oligosaccharide compound is then polymerized to an average degree of polymerization from 11 to 500 in the presence of an enzyme. Another method for producing the polysaccharide substance is provided in which an aldehyde group at the reducing terminal of an oligosaccharide having a degree of polymerization from 3 to 10 is oxidized with an oxidizing agent, and the saccharide chain of the oxidized product is polymerized to a degree of polymerization from 11 to 500 in the presence of an enzyme, followed by the addition of an acid. The polysaccharide substance has excellent solvent resistance and is useful as a separating agent for chromatography, especially for the separation of chiral compounds.

摘要翻译： 提供具有下式的多糖物质：还提供了一种多糖物质的制造方法，其中聚合度为3〜10的寡糖与硅烷试剂化学键合 所得寡糖的还原末端，然后在酶的存在下将寡糖化合物聚合至平均聚合度为11至500。 提供另一种生产多糖物质的方法，其中将聚合度为3-10的低聚糖的还原末端的醛基用氧化剂氧化，并将氧化产物的糖链聚合到一定程度 在酶存在下从11-500聚合，然后加入酸。 多糖物质具有优异的耐溶剂性，可用作色谱分离剂，特别是手性化合物的分离。