公开(公告)号：US5158891A

公开(公告)日：1992-10-27

申请号：US492227

申请日：1990-03-13

申请人： Yasuhiko Takeda ,  Mikio Fujii ,  Yukihiro Nakajo ,  Sadao Isshiki

发明人： Yasuhiko Takeda ,  Mikio Fujii ,  Yukihiro Nakajo ,  Sadao Isshiki

IPC分类号： C12N15/00 ,  C12N15/77

CPC分类号： C12N15/77 ,  Y10S435/843

摘要： A plasmid and a DNA fragment each of which contains a gene for tetracycline resistance derived from a glutamic acid-producing coryneform bacterium, and a coryneform bacterium containing said DNA fragment. The gene for tetracycline resistance derived from a glutamic acid-producing coryneform bacterium is a useful selective marker in effecting the breeding of a glutamic acid-producing coryneform bacterium by genetic recombination. Using the plasmid, the DNA fragment or the coryneform bacterium of the present invention, breeding of the glutamic acid-producing coryneform bacterium can be easily and effectively conducted by means of recombinant DNA technique.

摘要翻译： 一种质粒和一个DNA片段，每个含有产生谷氨酸的棒状细菌的四环素抗性基因，以及含有所述DNA片段的棒状细菌。 来自产生谷氨酸棒状杆菌的四环素抗性的基因是通过遗传重组实现产生谷氨酸的棒状细菌的育种的有用选择标记。 使用质粒，可以通过重组DNA技术容易且有效地进行本发明的DNA片段或棒状细菌的育种，产生谷氨酸的棒状细菌。