公开(公告)号：US07037672B2

公开(公告)日：2006-05-02

申请号：US10419095

申请日：2003-04-21

Applicant: Chiaki Saitoh ,  Atsushi Yashiro ,  Chikara Tokunaga ,  Kyoko Ozawa ,  Atsuko Yokoi ,  Nobuo Ogata ,  Hiroshi Katahira ,  Keiko Ochiai ,  Katsuhiko Ando

Inventor： Chiaki Saitoh ,  Atsushi Yashiro ,  Chikara Tokunaga ,  Kyoko Ozawa ,  Atsuko Yokoi ,  Nobuo Ogata ,  Hiroshi Katahira ,  Keiko Ochiai ,  Katsuhiko Ando

IPC: C12P1/00

CPC classification number: C12R1/15 ,  A23B7/10 ,  A23L27/24 ,  C12P17/04 ,  Y10S435/843

Abstract: The present invention relates to a novel flavoring solution for making pickles having a flavor of foods pickled in rice bran paste (hereinafter referred to as a bran pickles flavoring solution), a clear bran pickles flavoring solution, processes for producing said solutions, a novel microorganism belonging to the genus Corynebacterium used for the production of said flavoring solutions, and a process for producing a solution containing a lactone wherein the lactone is selectively extracted from a culture of a lactone-producing microorganism.The present invention also relates to a novel bacterium of the genus Corynebacterium which has the ability to produce γ-dodecalactone and/or γ-dodecelactone and is useful for the production of said bran pickles flavoring solutions.

公开(公告)号：US20030148498A1

公开(公告)日：2003-08-07

申请号：US10322455

申请日：2002-12-19

Inventor： Jeong-Hwan Kim ,  Young-Hyeon Kwag ,  Jang-Hee Park ,  Eun-Sung Koh ,  Yoon-Suk Oh ,  Jea-Young Chang ,  Kwang-Ho Lee ,  Jae-Ick Sim ,  Jong-Kwon Han ,  Young-Hoon Park

IPC: C12N001/12 ,  C12N001/20

CPC classification number: C12R1/15 ,  C12N1/20 ,  C12P19/32 ,  Y10S435/843

Abstract: The invention relates to microorganism producing 5null-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXPK001 which is a mutant strain of Corynebacterium ammoniagenes KFCC 10743 having a resistance to 3,4-dehydro-DL-proline. Since Corynebacterium ammoniagenes CJXPK001 of the present invention is resistant to 3,4-dehydro-DL-proline and is not affected by the osmotic pressure caused by the accumulated, highly concentrated solute in the medium, it can produce 5null-xanthylic acid at a high yield and concentration rate.

Abstract translation: 本发明涉及产生5'-戊酸的微生物。 更具体地，本发明涉及产氨棒杆菌CJXPK001，其是具有对3,4-脱氢-DL-脯氨酸的抗性的产氨棒杆菌KFCC 10743的突变株。 由于本发明的产氨酸棒杆菌CJXPK001对3,4-脱氢-DL-脯氨酸具有抗性，并且不受由培养基中积累的高度浓缩的溶质引起的渗透压的影响，所以可以产生5-x-戊酸 高产量和集中率。

公开(公告)号：US20030113878A1

公开(公告)日：2003-06-19

申请号：US10283075

申请日：2002-10-30

Inventor： Hungming J. Liaw ,  John Eddington ,  Yueqin Yang ,  Richard Dancey ,  Stacia Swisher ,  Weiying Mao

IPC: C12P019/04 ,  C12P013/04 ,  C12N015/74 ,  C12N001/21

CPC classification number: C12R1/15 ,  C12N15/01 ,  C12P13/08 ,  Y10S435/843

Abstract: The invention provides novel microorganisms, methods for the production thereof and novel processes for the production of amino acids. Mutagenesis of parental bacterial strains and selection of an improved raffinate-resistant phenotype enables the isolation of strains with enhanced growth properties that produce larger amounts of amino acid. Microorganisms of the invention are produced from amino acid producing parental strains such as Corynebacterium or Brevibacterium, particularly preferred are parental strains that produce L-lysine.

Abstract translation: 本发明提供新的微生物，其生产方法和制备氨基酸的新方法。 亲本细菌菌株的诱变和提高的残液抗性表型的选择使得能够分离产生更大量氨基酸的增强生长特性的菌株。 本发明的微生物由氨基酸生产的亲本菌株如棒状杆菌或短杆菌产生，特别优选的是产生L-赖氨酸的亲本菌株。

公开(公告)号：US5840551A

公开(公告)日：1998-11-24

申请号：US769437

申请日：1996-12-19

Applicant: Holger Werning ,  Harald Voss ,  Walter Pfefferle ,  Wolfgang Leuchtenberger

Inventor： Holger Werning ,  Harald Voss ,  Walter Pfefferle ,  Wolfgang Leuchtenberger

IPC: C12P13/04 ,  C12P13/08 ,  C12P13/06

CPC classification number: C12P13/04 ,  C12P13/08 ,  Y10S435/83 ,  Y10S435/832 ,  Y10S435/84 ,  Y10S435/843 ,  Y10S435/872

Abstract: A method of producing L-amino acids by fermentation. Microorganisms of the genus Corynebacterium which exhibit an auxotrophy relative to an amino acid are used as biocatalysts. The method is characterized in that the carbon source on the one hand and the limiting amino acid on the other hand are fed in two or more different infeed currents to the process. The infeed profiles have, for example, a concave (saccharose) and an exponential (amino acid) form or a convex (saccharose) and likewise a convex (amino acid) form, with specific differing degrees of increase of the currents relative to each other over time.

Abstract translation: 通过发酵生产L-氨基酸的方法。 显示出相对于氨基酸的营养缺陷型棒状杆菌属的微生物用作生物催化剂。 该方法的特征在于一方面碳源和限制性氨基酸以两种或多种不同的进料电流进料至该方法。 进料曲线具有例如凹（蔗糖）和指数（氨基酸）形式或凸状（蔗糖）和类似于凸（氨基酸）形式，其电流相对于彼此具有特定不同程度的增加 随着时间的推移。

公开(公告)号：US5763231A

公开(公告)日：1998-06-09

申请号：US610874

申请日：1996-03-05

Applicant: Yukiko Ono ,  Katsuaki Sato

Inventor： Yukiko Ono ,  Katsuaki Sato

IPC: C12P13/06 ,  C12R1/15 ,  C12R1/19 ,  C12P13/04

CPC classification number: C12P13/06 ,  Y10S435/843 ,  Y10S435/848 ,  Y10S435/849

Abstract: A process for producing L-leucine, which includes incubating an L-leucine-productive microorganism belonging to the genus Corynebacterium, Escherichia, Brevibacterium, or Microbacterium in a culture medium and reacting the resulting cells with saccharides and acetic acid or its salt to form and accumulate L-leucine in the reaction solution. The process improves the amount of L-leucine accumulated and decreases formation of amino acid byproducts.

Abstract translation: 一种生产L-亮氨酸的方法，其包括在培养基中孵育属于棒状杆菌属，埃希氏杆菌属，短杆菌属或微杆菌属的L-亮氨酸生产性微生物，并使所得细胞与糖和乙酸或其盐反应，形成和 在反应溶液中积聚L-亮氨酸。 该过程提高了L-亮氨酸的积累量，减少了氨基酸副产物的形成。

公开(公告)号：US5597727A

公开(公告)日：1997-01-28

申请号：US245688

申请日：1994-05-18

Applicant: Keiko Kohama ,  Kazuhisa Hatakeyama ,  Yasurou Kurusu ,  Hideaki Yukawa

Inventor： Keiko Kohama ,  Kazuhisa Hatakeyama ,  Yasurou Kurusu ,  Hideaki Yukawa

IPC: C12N1/21 ,  C12N9/88 ,  C12N15/09 ,  C12N15/69 ,  C12N15/77 ,  C12R1/13 ,  C12R1/15 ,  C07H21/04 ,  C12N15/00 ,  C12N15/74

CPC classification number: C12N9/88 ,  C12N15/69 ,  C12N15/77 ,  Y10S435/84 ,  Y10S435/843

Abstract: The present invention relates to a DNA fragment which contains a gene responsible for the function of autonomous replication of plasmid in a Coryneform bacterium, said gene being obtained from plasmid pBY503 held in Brevibacterium stationis, and in which at least one point mutation capable of increasing the copy number of plasmid exists on said gene region. By using a Coryneform transformed with the vector constructed using said DNA fragment and an industrially useful gene such as aspartase gene or tryptophan synthase gene, production of the useful product occurs with higher efficiency than conventional methods.

Abstract translation: 本发明涉及一种DNA片段，其含有负责棒状杆菌型细菌质粒自主复制功能的基因，所述基因得自持有短杆菌属的质粒pBY503，其中能够增加至少一个能够增加 所述基因区上存在质粒的拷贝数。 通过使用由使用所述DNA片段构建的载体和工业上有用的基因如天冬氨酸酶基因或色氨酸合成酶基因转化的棒状细胞，有效产物的产生以比常规方法更高的效率发生。

公开(公告)号：US5484716A

公开(公告)日：1996-01-16

申请号：US130995

申请日：1993-10-04

Applicant: Ryoichi Katsumata ,  Kuniki Kino

Inventor： Ryoichi Katsumata ,  Kuniki Kino

IPC: C12P13/22 ,  C12R1/13 ,  C12R1/15

CPC classification number: C12P13/22 ,  Y10S435/84 ,  Y10S435/843

Abstract: The invention relates to a bacterial process for producing L-tryptophan, L-tyrosine or L-phenylalanine. The process utilizes a coryneform glutamic acid-producing bacterium being capable of producing L-tryptophan, L-tyrosine or L-phenylalanine and also decreased or lacked in phosphoenolpyruvate carboxylase activity. The mutant strain is then cultured in order to accumulate the amino acid in a medium and the amino acid is recovered therefrom.

Abstract translation: 本发明涉及生产L-色氨酸，L-酪氨酸或L-苯丙氨酸的细菌方法。 该方法利用产生L-色氨酸，L-酪氨酸或L-苯丙氨酸的棒状谷氨酸生产细菌，并且还降低或缺乏磷酸烯醇丙酮酸羧化酶活性。 然后培养突变菌株以将氨基酸在培养基中积累，并从中回收氨基酸。

公开(公告)号：US5268293A

公开(公告)日：1993-12-07

申请号：US851120

申请日：1992-03-16

Applicant: Jong W. Oh ,  Seong J. Kim ,  Young J. Cho ,  Nai H. Park ,  Jae H. Lee

Inventor： Jong W. Oh ,  Seong J. Kim ,  Young J. Cho ,  Nai H. Park ,  Jae H. Lee

IPC: C12P13/08 ,  C12N1/20

CPC classification number: C12R1/15 ,  C12P13/08 ,  Y10S435/843

Abstract: Disclosed are Corynebacterium glutamicum CS-755 (KFCC 10672, FERM BP-2763), a L-lysine producing microorganism which is resistant to .alpha.-amino-.beta.-hydroxyvaleric acid, S-(.beta.-aminoethyl)-L-cysteine, methyl lysine, arginine analogues, other analogues, .beta.-(2 -thiazolyl)-DL-alanine, 5-hydroxyuridine, 6-azauracil, and 6-fluorotryptophan requires leucine and homoserine for growth, and a method for producing L-lysine comprising culturing the strain (KFCC 10672, FERM BP-2763) and recovering L-lysine from the resultant culture broth.

Abstract translation: 公开了谷氨酸棒杆菌CS-755（KFCC 10672，FERM BP-2763），对α-氨基-β-羟基戊酸具有抗性的L-赖氨酸生产微生物，S-（β-氨基乙基）-L-半胱氨酸，甲基赖氨酸 ，精氨酸类似物，其他类似物，β-（2-噻唑基）-DL-丙氨酸，5-羟基尿苷，6-氮尿嘧啶和6-氟色氨酸需要亮氨酸和高丝氨酸进行生长，以及生产L-赖氨酸的方法，包括培养菌株 （KFCC 10672，FERM BP-2763）并从所得培养液中回收L-赖氨酸。

公开(公告)号：US5250434A

公开(公告)日：1993-10-05

申请号：US702111

申请日：1991-05-16

Applicant: Kazuhiko Yamada ,  Akira Seto

Inventor： Kazuhiko Yamada ,  Akira Seto

IPC: C12P13/14 ,  C12N1/20

CPC classification number: C12R1/15 ,  C12P13/14 ,  Y10S435/843

Abstract: Novel bacteria identified as effective in glutamic acid production through fermentation chemistry conducted at temperatures above 42.degree. C.

Abstract translation: 通过在42℃以上的温度下进行的发酵化学鉴定的新型细菌在谷氨酸生产中有效。

公开(公告)号：US5240835A

公开(公告)日：1993-08-31

申请号：US418617

申请日：1989-10-10

Applicant: Frank A. Pettrone ,  Patrick J. Grisdale ,  Gregory M. Whited ,  Theresa C. Paulson

Inventor： Frank A. Pettrone ,  Patrick J. Grisdale ,  Gregory M. Whited ,  Theresa C. Paulson

IPC: C12N9/18 ,  C12N9/78 ,  C12P7/62

CPC classification number: C12N9/78 ,  C12N9/18 ,  C12P7/62 ,  Y10S435/843

Abstract: Unsaturated esters can be converted into unsaturated polymerizable monomers using a biocatalyst derived from Corynebacterium oxydans. The method involves the step of reacting an unsaturated ester with an organic compound having a primary or secondary hydroxy group in a substantially organic environment in the presence of the noted biocatalyst. A transacylase has been isolated from the microorganism and at least partially purified.

Abstract translation: 不饱和酯可以使用衍生自棒状杆菌的生物催化剂转化成不饱和可聚合单体。 该方法包括在所述生物催化剂存在下使不饱和酯与具有伯或仲羟基的有机化合物在基本有机环境中反应的步骤。 从微生物中分离出转酰胺酶，并至少部分纯化。