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    Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
    1.
    发明授权
    Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media 有权
    使用阴离子交换介质从体液分离核酸的组合物，方法和试剂盒

    公开(公告)号：US09163229B2

    公开(公告)日：2015-10-20

    申请号：US11974016

    申请日：2007-10-10

    申请人： Hovsep Melkonyan ,  Erik Meyer ,  William John Feaver ,  Samuil R. Umansky

    发明人： Hovsep Melkonyan ,  Erik Meyer ,  William John Feaver ,  Samuil R. Umansky

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68

    CPC分类号： C12N15/101 ,  C12N15/1006 ,  C12N15/1017 ,  C12Q1/6806

    摘要： This invention provides compositions and methods for rapid isolation and purification of nucleopolymers from biological samples using anionic exchange media. The method of this invention can utilize commercially available anion exchanger materials with selected solutions of known ionic strength for absorption and elution. The medium/nucleoprotein bound complex may be optionally stored or transported, and is subsequently treated with eluents to remove undesirable proteins and inorganic salts. The partially purified complex may also be stored or transported. This method is particularly advantageous since it allows rapid isolation and purification of soluble nucleic acids from a large volume of biological fluids with easy handling and storage, stabilizing the samples against degradation prior to analysis. It also permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, until now, had not been identified. The method is widely useful to prepare samples formats that are convenient in diagnostic analytical methods.

    摘要翻译： 本发明提供了使用阴离子交换介质从生物样品快速分离和纯化核聚合物的组合物和方法。 本发明的方法可以利用具有已知离子强度的所选溶液进行吸收和洗脱的市售阴离子交换剂材料。 中等/核蛋白结合的复合物可以任选地储存或运输，随后用洗脱液处理以除去不需要的蛋白质和无机盐。 部分纯化的复合物也可以被储存或运输。 该方法是特别有利的，因为其允许从易于处理和储存的大量生物流体中快速分离和纯化可溶性核酸，从而在样品分析之前使样品不会降解。 它还允许纯化和鉴定来自身体液体的较短的核酸片段，迄今为止尚未鉴定。 该方法广泛用于准备在诊断分析方法中方便的样品格式。

    METHOD FOR DIAGNOSIS AND MONITORING OF VIRAL INFECTION BY ANALYSIS OF VIRAL TRANSRENAL NUCLEIC ACIDS IN URINE
    2.
    发明申请
    METHOD FOR DIAGNOSIS AND MONITORING OF VIRAL INFECTION BY ANALYSIS OF VIRAL TRANSRENAL NUCLEIC ACIDS IN URINE 审中-公开
    尿毒症病毒分子核酸分析法检测和监测病毒感染的方法

    公开(公告)号：US20130178544A1

    公开(公告)日：2013-07-11

    申请号：US13777946

    申请日：2013-02-26

    申请人： Hovsep S. MELKONYAN ,  Angela Cannas ,  Louis David Tomei ,  Samuil R. Umansky

    发明人： Hovsep S. MELKONYAN ,  Angela Cannas ,  Louis David Tomei ,  Samuil R. Umansky

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/706 ,  C12Q1/70 ,  C12Q1/703 ,  Y02A50/58 ,  Y10S435/81

    摘要： The present invention relates to methods for diagnosis or monitoring of viral infection by detecting the presence of transrenal viral nucleic acids or nucleic acids of viral origin in urine sample, with or without isolation of nucleic acids from a urine sample. The analysis of the nucleic acids is performed through hybridization of the nucleic acids with specific probes, or through a chain amplification reaction with specific primers. The methods are applicable to all viral pathogenic agents, including RNA, DNA, episomal, or integrated viruses.

    摘要翻译： 本发明涉及用于通过检测在尿样中是否存在来自尿液样本的病毒来源的经肾上腺病毒核酸或核酸的存在来诊断或监测病毒感染的方法，其中或不分离来自尿液样品的核酸。 核酸的分析通过核酸与特异性探针的杂交，或通过与特异性引物的链扩增反应来进行。 所述方法适用于所有病毒致病剂，包括RNA，DNA，附加型或综合病毒。

    Compositions and Methods for Detecting Pathogen Specific Nucleic Acids in Urine
    3.
    发明申请
    Compositions and Methods for Detecting Pathogen Specific Nucleic Acids in Urine 审中-公开
    用于检测尿液中病原体特异性核酸的组合物和方法

    公开(公告)号：US20120021412A1

    公开(公告)日：2012-01-26

    申请号：US13029308

    申请日：2011-02-17

    申请人： Hovsep Melkonyan ,  Angela Cannas ,  Louis David Tomei ,  Samuil R. Umansky

    发明人： Hovsep Melkonyan ,  Angela Cannas ,  Louis David Tomei ,  Samuil R. Umansky

    IPC分类号： C12Q1/68

    CPC分类号： C07H21/04 ,  C12N15/1006 ,  Y02A50/55

    摘要： The invention is based upon the discovery that small nucleic acids from non-viral pathogens are able to cross the kidney and are present in urine of a subject when the subject is infected with the non-viral pathogen. These transrenal DNAs are especially prevalent at smaller sizes under about 300 bp. Thus the invention provides compositions and methods for the diagnosis of infection of a subject with non-viral pathogens through the detection of transrenal nucleic acids from those pathogens in the urine of the subject.

    摘要翻译： 本发明基于以下发现：当受试者感染非病毒病原体时，来自非病毒病原体的小核酸能够穿过肾脏并存在于受试者的尿液中。 这些跨肾脏DNA在大约300bp的较小尺寸下是特别普遍的。 因此，本发明提供了通过检测受试者尿液中的那些病原体的经肾上腺核酸来诊断患有非病毒病原体的受试者的感染的组合物和方法。

    Genetic marker for detection of human papillomavirus
    4.
    发明授权
    Genetic marker for detection of human papillomavirus 有权
    用于检测人乳头状瘤病毒的遗传标记

    公开(公告)号：US08642261B2

    公开(公告)日：2014-02-04

    申请号：US13126081

    申请日：2009-10-26

    申请人： Samuil R. Umansky ,  Zhenghan Xin ,  Natalya Ossina

    发明人： Hovsep S. Melkonyan ,  Samuil R. Umansky ,  Zhenghan Xin

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/708 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16

    摘要： The invention provides compositions and methods for the differential detection of high risk forms of HPV from a urine sample provided by a patient. Specifically, the invention provides primers and probes that specifically recognize and bind sequences within the E1 gene of HPV. Detection of high risk forms of HPV identify individuals at risk of developing or in the early stages of cervical carcinoma.

    摘要翻译： 本发明提供了用于从患者提供的尿样中差异检测HPV的高风险形式的组合物和方法。 具体地说，本发明提供了特异性识别和结合HPV基因E1基因序列的引物和探针。 检测HPV的高风险形式识别患有发展或宫颈癌早期发生危险的个体。

    Method for Diagnosis and Monitoring of Viral Infection by Analysis of Viral Transrenal Nucleic Acids in Urine
    5.
    发明申请
    Method for Diagnosis and Monitoring of Viral Infection by Analysis of Viral Transrenal Nucleic Acids in Urine 有权
    通过尿液中病毒性肾上腺核酸分析病毒感染的诊断和监测方法

    公开(公告)号：US20120021404A1

    公开(公告)日：2012-01-26

    申请号：US13114289

    申请日：2011-05-24

    申请人： Hovsep Melkonyan ,  Angela Cannas ,  Louis David Tomei ,  Samuil R. Umansky

    发明人： Hovsep Melkonyan ,  Angela Cannas ,  Louis David Tomei ,  Samuil R. Umansky

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/706 ,  C12Q1/70 ,  C12Q1/703 ,  Y02A50/58 ,  Y10S435/81

    摘要： The present invention relates to methods for diagnosis or monitoring of viral infection by detecting the presence of transrenal viral nucleic acids or nucleic acids of viral origin in urine sample, with or without isolation of nucleic acids from a urine sample. The analysis of the nucleic acids is performed through hybridization of the nucleic acids with specific probes, or through a chain amplification reaction with specific primers. The methods are applicable to all viral pathogenic agents, including RNA, DNA, episomal, or integrated viruses.

    摘要翻译： 本发明涉及用于通过检测在尿样中是否存在来自尿液样本的病毒来源的经肾上腺病毒核酸或核酸的存在来诊断或监测病毒感染的方法，其中或不分离来自尿液样品的核酸。 核酸的分析通过核酸与特异性探针的杂交，或通过与特异性引物的链扩增反应来进行。 所述方法适用于所有病毒致病剂，包括RNA，DNA，附加型或综合病毒。