公开(公告)号：US08663909B2

公开(公告)日：2014-03-04

申请号：US11109857

申请日：2005-04-20

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： C12Q1/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12M1/36 ,  C12M1/38 ,  C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  C12N11/00 ,  C12N11/16 ,  G01N33/53 ,  G01N33/569 ,  G01N33/554 ,  G01N33/543

CPC分类号： C12Q1/04

摘要： This invention describes a device consisting of a micro channel plate, filter, and porous holder for filter, which is substituted by a pure agar block during method performance, and supportive structural elements. The device is intended for rapid detection and/or identification of microorganisms. Microorganisms are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides and attached to a filter from one side. A micro channel plate houses a multiplicity of micro channels (possible diameter of each channel=1-30 μm, length 100-1000 μm, and number on centimeter2=100,000-1,000,000). The micro channel plate with cells trapped on the surface of the filter is attached to an agar block impregnated by artificial substrate(s) so that the molecules of the artificial substrates will fill all micro channels. Trapped cells produce colored or fluorescent molecules from artificial substrates. These molecules are collected in the very small volume of a micro channel. The extremely small volume of a micro channel (1/25 million part of milliliter) allows it to collect a detectable concentration of color or fluorescent substances in a very short time (several minutes). Even one cell from a filtrated sample can be detected by the enzyme—artificial substrate method and/or identified by enzyme immunoassay.

摘要翻译： 本发明描述了一种由微通道板，过滤器和用于过滤器的多孔保持器组成的装置，其在方法执行期间由纯琼脂块代替，并且支撑结构元件。 该装置旨在快速检测和/或鉴定微生物。 微生物被从两侧敞开的长（直径/长度= 1 / 10-1 / 100），圆柱形，平行的微通道中过滤，并从一侧连接到过滤器上。 微通道板容纳多个微通道（每个通道的可能直径=1-30μm，长度为100-1000μm，数量为厘米2 = 100,000-1,000,000）。 将具有捕获在过滤器表面上的细胞的微通道板连接到由人造底物浸渍的琼脂块上，使得人造底物的分子将填充所有微通道。 被捕获的细胞从人造底物产生着色或荧光分子。 这些分子被收集在微通道的非常小体积中。 微通道的体积非常小（1/25百万份毫升）可以在很短的时间（几分钟）内收集可检测浓度的颜色或荧光物质。 甚至可以通过酶 - 人造底物法检测来自过滤样品的一个细胞和/或通过酶免疫测定来鉴定。

公开(公告)号：US20130089887A1

公开(公告)日：2013-04-11

申请号：US13269798

申请日：2011-10-10

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： C12Q1/04 ,  C12M1/34

CPC分类号： C12M41/36 ,  C12Q1/045

摘要： This invention describes method for rapid detection and identification of colonies and micro-colonies of microorganisms growing on the surface of certain nutrient agar under certain porous material(s) impregnated by chromogenic and/or flurogenic substrates and supportive substances. Growth in the space between nutrient agar and impregnated porous material allows limiting the negative influence of unfavorable substrates and substances and allows to reveal color or fluorescence spots at early stage of micro-colony growth.

摘要翻译： 本发明描述了用于在由显色和/或氟化底物和支持物质浸渍的某些多孔材料下快速检测和鉴定生长在某些营养琼脂的表面上的微生物的菌落和微生物菌群的方法。 在营养琼脂和浸渍多孔材料之间的空间中的生长允许限制不利的底物和物质的负面影响，并允许在微生殖生长的早期阶段显示颜色或荧光斑点。

公开(公告)号：US20160106117A1

公开(公告)日：2016-04-21

申请号：US14515026

申请日：2014-10-15

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： A23C9/12 ,  C12M1/00 ,  C12M3/00 ,  C12M1/12

CPC分类号： A23C9/122

摘要： Portable reusable fermenter for easy preparation of homemade kefir with use of kefir grains restrained in the plastic chamber submerged in the milk. Chamber has large number of halls for easy access of milk to kefir grains. The size of halls is significantly smaller that the size of kefir grains in order to keep grains inside chamber but big enough for free access to milk. Portable fermenter with the same set of grains is reusable. Drinking of kefir can be done through plastic straw without of separation of grains and ready kefir.

摘要翻译： 便携式可重复使用的发酵罐，用于容易地制备自制牛皮纸，其中使用含牛皮糖的蛋白质，在浸没在牛奶中的塑料室中被限制。 商会有大量的礼堂可以方便地将牛奶加入牛肉饼。 大厅的大小明显小于牛肉粒的大小，以保持谷物在室内，但足够大可以自由进入牛奶。 具有相同组谷物的便携式发酵罐是可重复使用的。 可以通过塑料秸秆进行腌制，而不会分离谷物和熟食。

公开(公告)号：US20100047850A1

公开(公告)日：2010-02-25

申请号：US12430866

申请日：2009-04-27

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： C12Q1/24

CPC分类号： C12Q1/04

摘要： This invention describes a method of rapid detection of micro-colonies of microorganisms by changing their shape from a regular semi-sphere to a long and thin cylinder. Cells are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides, and attached to a filter from one side. A micro-channel plate houses a multiplicity of micro-channels (diameter of each channel=1-20 μm, and length 100-1000 μm). The micro-channel plate with cells trapped on the surface of the filter is attached to a nutrient media agar block. Cells produce micro-colonies of a long and thin shape according the shape of the micro-channel. The growth of microorganisms in the micro-channels permits a change in the number of cells to accomplish light absorbance. Fewer cells need a shorter time to reproduce. Thus detection and counting of cells can be accomplished in a rapid fashion. The light absorbance can be enhanced by additional coloration of micro-colonies by cell dyes or artificial chromogenic or fluorogenic substrates.

摘要翻译： 本发明描述了一种通过将它们的形状从规则的半球改变成长而薄的圆筒来快速检测微生物微生物的方法。 通过从两侧敞开的长（直径/长度= 1 / 10-1 / 100），圆柱形，平行的微通道过滤来捕获细胞，并从一侧连接到过滤器。 微通道板容纳多个微通道（每个通道的直径=1-20μm，长度为100-1000μm）。 将具有捕获在过滤器表面上的细胞的微通道板连接到营养培养基琼脂块上。 细胞根据微通道的形状产生长而薄的微细菌落。 微通道中微生物的生长允许细胞数量的改变以实现光吸收。 更少的细胞需要更短的时间来繁殖。 因此，细胞的检测和计数可以以快速的方式完成。 可以通过细胞染料或人造色素或荧光底物的微集落的附加着色来增强光吸收。

公开(公告)号：US07524623B2

公开(公告)日：2009-04-28

申请号：US11393012

申请日：2006-03-30

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： C12Q1/00 ,  C12Q1/02 ,  C12N11/00

CPC分类号： C12Q1/04

摘要： This invention describes a method of rapid detection of micro-colonies of microorganisms by changing their shape from a regular semi-sphere to a long and thin cylinder. Cells are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides, and attached to a filter from one side. A micro-channel plate houses a multiplicity of micro-channels (diameter of each channel=1 -20 μm, and length 100-1000 μm). The micro-channel plate with cells trapped on the surface of the filter is attached to a nutrient media agar block. Cells produce micro-colonies of a long and thin shape according the shape of the micro-channel. The growth of microorganisms in the micro-channels permits a change in the number of cells to accomplish light absorbance. Fewer cells need a shorter time to reproduce. Thus detection and counting of cells can be accomplished in a rapid fashion. The light absorbance can be enhanced by additional coloration of micro-colonies by cell dyes or artificial chromogenic or fluorogenic substrates.

摘要翻译： 本发明描述了一种通过将它们的形状从规则的半球改变成长而薄的圆筒来快速检测微生物微生物的方法。 通过从两侧敞开的长（直径/长度= 1 / 10-1 / 100），圆柱形，平行的微通道过滤来捕获细胞，并从一侧连接到过滤器。 微通道板容纳多个微通道（每个通道的直径= 1〜20μm，长度为100-1000μm）。 将具有捕获在过滤器表面上的细胞的微通道板连接到营养培养基琼脂块上。 细胞根据微通道的形状产生长而薄的微细菌落。 微通道中微生物的生长允许细胞数量的改变以实现光吸收。 更少的细胞需要更短的时间来繁殖。 因此，细胞的检测和计数可以以快速的方式完成。 可以通过细胞染料或人造色素或荧光底物的微集落的附加着色来增强光吸收。

公开(公告)号：US20070238139A1

公开(公告)日：2007-10-11

申请号：US11393012

申请日：2006-03-30

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： G01N33/52

CPC分类号： C12Q1/04

摘要： This invention describes a method of rapid detection of micro-colonies of microorganisms by changing their shape from a regular semi-sphere to a long and thin cylinder. Cells are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides, and attached to a filter from one side. A micro-channel plate houses a multiplicity of micro-channels (diameter of each channel=1 -20 μm, and length 100-1000 μm). The micro-channel plate with cells trapped on the surface of the filter is attached to a nutrient media agar block. Cells produce micro-colonies of a long and thin shape according the shape of the micro-channel. The growth of microorganisms in the micro-channels permits a change in the number of cells to accomplish light absorbance. Fewer cells need a shorter time to reproduce. Thus detection and counting of cells can be accomplished in a rapid fashion. The light absorbance can be enhanced by additional coloration of micro-colonies by cell dyes or artificial chromogenic or fluorogenic substrates.

摘要翻译： 本发明描述了一种通过将它们的形状从规则的半球改变成长而薄的圆筒来快速检测微生物微生物的方法。 通过从两侧敞开的长（直径/长度= 1 / 10-1 / 100），圆柱形，平行的微通道过滤来捕获细胞，并从一侧连接到过滤器。 微通道板容纳多个微通道（每个通道的直径= 1〜20μm，长度为100-1000μm）。 将具有捕获在过滤器表面上的细胞的微通道板连接到营养培养基琼脂块上。 细胞根据微通道的形状产生长而薄的微细菌落。 微通道中微生物的生长允许细胞数量的改变以实现光吸收。 更少的细胞需要更短的时间来繁殖。 因此，细胞的检测和计数可以以快速的方式完成。 可以通过细胞染料或人造色素或荧光底物的微集落的附加着色来增强光吸收。

公开(公告)号：US20130288294A1

公开(公告)日：2013-10-31

申请号：US13869650

申请日：2013-04-24

申请人： Michael T. Saul ,  Sergey Gazenko

发明人： Michael T. Saul ,  Sergey Gazenko

IPC分类号： C12Q1/04

CPC分类号： C12Q1/045 ,  C12N1/20 ,  C12Q1/04 ,  G01N2333/21

摘要： A method and point of use kit to determine under field conditions the presence and/or amount of a selected Pseudomonas species capable of bioremediating organic environmental pollutants, such as petroleum hydrocarbons.

公开(公告)号：US08361783B2

公开(公告)日：2013-01-29

申请号：US12797106

申请日：2010-06-09

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： C12M1/34

CPC分类号： C12Q1/04

摘要： The present invention provides a device such as a medical device for the rapid detection, enumeration and identification of microorganisms. It is based on the production and accumulation of absorbent or fluorescent molecules during reactions between artificial substrates and enzymes in micro-channels of a sampling-detecting unit of the device. Enzymes of cells, or enzymes attached to cell bodies through antibody-enzyme conjugates, produce easily detectable concentrations of colored or fluorescent molecules in very small volumes, much faster than what is produced in conventional large volume devices. Microorganisms contained in the micro-channels appear as colored or fluorescent dots when viewed using a light or a fluorescent microscope.

摘要翻译： 本发明提供了一种诸如用于微生物的快速检测，计数和鉴定的医疗装置的装置。 它基于在装置的采样检测单元的微通道中人造基底和酶之间的反应期间吸收剂或荧光分子的产生和积累。 细胞的酶或通过抗体 - 酶缀合物连接到细胞体的酶可以以非常小的体积产生容易检测到的有色或荧光分子浓度，比常规大容量装置中产生的快得多。 当使用光或荧光显微镜观察时，微通道中包含的微生物显示为有色或荧光点。

公开(公告)号：US20100190204A1

公开(公告)日：2010-07-29

申请号：US12532501

申请日：2008-03-24

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： C12Q1/06 ,  C12Q1/02 ,  C12N1/04 ,  C12M1/34

CPC分类号： C12Q1/04

摘要： This invention describes rapid detection and identification of colonies or micro-colonies of microorganisms after regular or short (several hours) growth periods on light pellucid, molecule-permeable membranes installed on solid nutrient media. Colonies or micro-colonies appearing on a membrane can be easily transferred from a growth plate to another media such as, pure agar or paper filled with indicator substances or substrates. Filterable and non-filterable samples can be analyzed by this method. A multitude of different methods of detection and identification can be realized using this invention in a micro-colony format: detection and enumeration of all live cells or specific live cells; detection and simultaneous identification of antibiotic-resistant microorganisms; different immunological methods of detection; detection and enumeration using machine analysis such as automated image identifiers.

摘要翻译： 本发明描述了在固体营养培养基上安装的透明透明的分子可渗透膜上，在常规或短（几个小时）生长期之后快速检测和鉴定微生物的菌落或微菌落。 出现在膜上的殖民地或微菌落可以容易地从生长板转移到另外的介质，例如填充有指示剂物质或底物的纯琼脂或纸。 可以通过这种方法分析可过滤和不可过滤的样品。 使用本发明可以以微团体形式实现多种不同的检测和鉴定方法：检测和计数所有活细胞或特定活细胞; 检测和同时鉴定抗生素抗性微生物; 不同的免疫学检测方法; 检测和枚举使用机器分析，如自动图像标识符。

公开(公告)号：US20080057562A1

公开(公告)日：2008-03-06

申请号：US11850774

申请日：2007-09-06

申请人： Sergey Gazenko

发明人： Sergey Gazenko

IPC分类号： C12N1/00 ,  C12M1/22 ,  C12M1/34

CPC分类号： C12M23/34 ,  C12M23/10

摘要： The present invention provides an apparatus comprised of devices for simple, fast and cost-effective storage and plating of solid nutrient medium (SNM) for various uses in the field of microbiological analysis. The devices consist of a container which can be aseptically filled with SNM and equipped with a plunger to accurately press out needed amounts of the SNM; a cutting means to cut, hold and transfer SNM; a measuring means to measure the amount of SNM exiting the container; and a growth plate for storage of SNM, growth of microorganisms on SNM, and introduction of indicator substances to SNM for analysis of microorganisms.

摘要翻译： 本发明提供一种装置，其包括用于在微生物分析领域中用于各种用途的固体营养培养基（SNM）的简单，快速和成本有效的储存和电镀的装置。 该装置由可以无菌地装满SNM并装有柱塞的容器组成，以准确地挤出所需量的SNM; 切割，保持和转移SNM的切割手段; 测量装置，用于测量离开容器的SNM的量; 以及用于储存SNM的生长板，微生物在SNM上的生长，以及向SNM引入指示剂物质以分析微生物。