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公开(公告)号：US07732174B2
公开(公告)日：2010-06-08
申请号：US10543866
申请日：2003-10-01
摘要： A method of using sequential chemostat culture vessels to provide continuous H2 production, in which photosynthetic O2 evolution and H2 photoproduction are separated physically into two separate bioreactors, comprising: a) growing a microorganism culture able to continuously generate H2 by photosynthetically producing cells at about the early-to-late log state in a first photobioreactor operating as a sulfur chemostat under aerobic and/or conditions; b) continuously feeding cells from the first photobioreactor to a second photobioreactor operating under anaerobic conditions and sulfur deprivation conditions resulting from constant uptake of sulfate in the first bioreactor and a low rate of culture flow between the first and second bioreactors, for induction of hydrogenase and H2 photoproduction to allow for continuous cultivation of the microorganism's cells in the first photobioreactor and constant H2 production in the second photobioreactor, and c) H2 gas from the second photobioreactor.
摘要翻译： 一种使用顺序恒化器培养容器提供连续H 2生产的方法，其中光合O 2进化和H 2光生产在物理上分离成两个单独的生物反应器，其包括：a）生长能够通过光合生产细胞在约 在有氧和/或条件下作为硫化学恒化器运行的第一个光生物反应器中的早期到晚期对数状态; b）将细胞从第一光生物反应器连续供给到在厌氧条件下操作的第二光生物反应器和由第一生物反应器中的硫酸盐持续摄取而产生的硫剥夺条件以及第一和第二生物反应器之间的较低培养流速，用于诱导氢化酶和 H2光生产以允许在第一光生物反应器中连续培养微生物细胞并在第二光生物反应器中恒定生成H2，以及c）来自第二光生物反应器的H2气。
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公开(公告)号：US20070269874A1
公开(公告)日：2007-11-22
申请号：US10543866
申请日：2003-10-01
申请人： Sergey Kosourov , Maria Ghirardi , Michael Seibert
发明人： Sergey Kosourov , Maria Ghirardi , Michael Seibert
IPC分类号： C12P1/04
摘要： A method of using sequential chemostat culture vessels to provide continuous H2 production, in which photosynthetic O2 evolution and H2 photoproduction are separated physically into two separate bioreactors, comprising: a) growing a microorganism culture able to continuously generate H2 by photosynthetically producing cells at about the early-to-late log state in a first photobioreactor operating as a sulfur chemostat under aerobic and/or conditions; b) continuously feeding cells from the first photobioreactor to a second photobioreactor operating under anaerobic conditions and sulfur deprivation conditions resulting from constant uptake of sulfate in the first bioreactor and a low rate of culture flow between the first and second bioreactors, for induction of hydrogenase and H2 photoproduction to allow for continuous cultivation of the microorganism's cells in the first photobioreactor and constant H2 production in the second photobioreactor, and c) H2 gas from the second photobioreactor.
摘要翻译： 使用顺序恒化器培养容器提供连续H 2 O 2生产的方法，其中将光合作用的O 2进化和H 2光生产物理地分离成 两个单独的生物反应器，其包括：a）生长微生物培养物，其能够在有氧时在硫化学恒化器上操作的第一光生物反应器中以大约早期到晚期的对数状态光合生产细胞连续产生H 2 N 2 和/或条件; b）将细胞从第一光生物反应器连续供给到在厌氧条件下操作的第二光生物反应器和由第一生物反应器中的硫酸盐持续摄取而产生的硫剥夺条件以及第一和第二生物反应器之间的较低培养流速，用于诱导氢化酶和 H 2光生产以允许第一光生物反应器中的微生物细胞的连续培养和第二光生物反应器中的恒定H 2 N 2生产，以及c）H 2 / 来自第二光生物反应器的气体。
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