-
1.发明授权公开(公告)号：US07452664B2
公开(公告)日：2008-11-18
申请号：US09461580
申请日：1999-12-15
CPC分类号： G01N33/66 , A61K38/1709 , C12Q1/48 , G01N2500/00 , Y02A50/473 , A61K2300/00
摘要： Methods of identifying agents which alter the NAD-dependent acetylation status and mono-ADP-ribosylation of nuclear proteins are disclosed. The methods further include identifying agents which alter the life span or aging of a cell or an organism by determining the level of NAD-dependent acetylation and/or ADP ribosylation of a nuclear protein. The invention also relates to a mammalian Sir2 protein which acetylates or deacetylates nuclear proteins in a NAD-dependent manner and has mono-ADP-ribosyltransferase activity. Host cells producing the Sir2 protein and antibodies to the Sir2 protein are also provided.
摘要翻译： 公开了鉴定改变核蛋白的NAD依赖性乙酰化状态和单ADP-核糖基化的试剂的方法。 所述方法还包括通过确定核蛋白的NAD依赖性乙酰化和/或ADP核糖基化的水平来鉴定改变细胞或生物体的寿命或衰老的试剂。 本发明还涉及哺乳动物Sir2蛋白，其以NAD依赖性方式乙酰化或脱乙酰化核蛋白并具有单ADP-核糖基转移酶活性。 还提供了产生Sir2蛋白的宿主细胞和Sir2蛋白的抗体。
-
公开(公告)号：US08268575B2
公开(公告)日：2012-09-18
申请号：US11575605
申请日：2005-09-20
IPC分类号： C12P19/36 , C07H21/04 , G01N33/573 , C12N15/63 , C12N5/10
CPC分类号： C12P19/36 , C12N9/1077
摘要： The present invention generally relates to a nicotinamide adenine dinucleotide (NAD) biosynthesis system and methods of screening for NAD biosynthesis effectors. Among the various aspects of the present invention is the provision of an in vitro-reconstituted mammalian NAD biosynthesis system that can be used for the high-throughput screening of chemical activators and inhibitors for mammalian NAD biosynthesis. Another aspect of the invention provides a method of identifying a compound that effects in vivo activity of NAD metabolic enzymes. Further aspects of the invention include nucleic acid sequences, vectors, and transformed cells that can be used in the methods described herein.
摘要翻译： 本发明一般涉及烟酰胺腺嘌呤二核苷酸（NAD）生物合成系统和筛选NAD生物合成效应物的方法。 在本发明的各个方面中，提供体外重构的哺乳动物NAD生物合成系统，其可用于化学活化剂的高通量筛选和用于哺乳动物NAD生物合成的抑制剂。 本发明的另一方面提供了鉴定影响NAD代谢酶的体内活性的化合物的方法。 本发明的其它方面包括可用于本文所述方法的核酸序列，载体和转化的细胞。
-
公开(公告)号：US20100105036A1
公开(公告)日：2010-04-29
申请号：US12408575
申请日：2009-03-20
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： This invention relates to methods of screening compounds that modulate cellular and organismal processes by modification of the activity of SIR2 and/or transcription factors, e.g., p53, particularly methods of screening for compounds that modify lifespan and/or metabolism of a cell or an organism by modulation of the activity of SIR2 and/or transcription factors, e.g., p53, and more particularly to methods of screening for compounds that modulate the activity of Sir2 and/or transcription factors, e.g., p53. In particular, the present invention relates to a method for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound. The invention further relates to therapeutic uses of said compounds. The invention further relates to a method of modifying the acetylation status of a transcription factor binding site on histone or DNA by raising local concentrations of Sir2.
摘要翻译： 本发明涉及通过修饰SIR2和/或转录因子活性（例如p53）来筛选调节细胞和生物体过程的化合物的方法，特别是筛选修饰细胞或生物体的寿命和/或代谢的化合物的方法 通过调节SIR2和/或转录因子例如p53的活性，更具体地涉及筛选调节Sir2和/或转录因子例如p53的活性的化合物的方法。 特别地，本发明涉及通过提供包含转录因子Sir2和Sir2辅因子与该化合物的测试混合物来筛选化合物的方法，并且在存在下评价测试混合物的组分的活性 化合物。 本发明还涉及所述化合物的治疗用途。 本发明还涉及通过提高Sir2的局部浓度来改变组蛋白或DNA上的转录因子结合位点的乙酰化状态的方法。
-
公开(公告)号：US20060252076A1
公开(公告)日：2006-11-09
申请号：US11404146
申请日：2006-04-13
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： This invention relates to methods of screening compounds that modulate cellular and organismal processes by modification of the activity of SIR2 and/or transcription factors, e.g., p53, particularly methods of screening for compounds that modify lifespan and/or metabolism of a cell or an organism by modulation of the activity of SIR2 and/or transcription factors, e.g., p53, and more particularly to methods of screening for compounds that modulate the activity of Sir2 and/or transcription factors, e.g., p53. In particular, the present invention relates to a method for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound. The invention further relates to therapeutic uses of said compounds. The invention further relates to a method of modifying the acetylation status of a transcription factor binding site on histone or DNA by raising local concentrations of Sir2.
-
5.发明授权Methods for identifying agents that alter NAD-dependent deacetylation activity of a SIR2 protein 有权用于鉴定改变SIR2蛋白的NAD依赖性脱乙酰活性的试剂的方法公开(公告)号：US08642284B1
公开(公告)日：2014-02-04
申请号：US09735786
申请日：2000-12-13
CPC分类号： C12Q1/48 , G01N2333/98 , G01N2440/10 , G01N2500/00
摘要： Methods of identifying agents which alter the NAD-dependent deacetylation activity of a Sir2 protein or a fragment of a Sir2 protein are disclosed. The acetylated protein can be a nuclear protein, such as a histone protein, or a cytoplasmic protein. The Sir2 protein employed in the methods can include at least a core domain of a Sir2 protein, such as a human Sir2 protein.
摘要翻译： 公开了鉴定改变Sir2蛋白或Sir2蛋白片段的NAD依赖性脱乙酰活性的试剂的方法。 乙酰化蛋白可以是核蛋白，例如组蛋白，或细胞质蛋白。 用于该方法的Sir2蛋白质可以包括Sir2蛋白质的至少核心结构域，例如人Sir2蛋白质。
-
公开(公告)号：US08546074B2
公开(公告)日：2013-10-01
申请号：US13523412
申请日：2012-06-14
IPC分类号： C12Q1/00 , G01N33/53 , G01N33/567
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： Methods for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound are described.
摘要翻译： 描述了通过向化合物提供包含转录因子Sir2和Sir2辅因子的测试混合物并在化合物存在下评价测试混合物的组分的活性来筛选化合物的方法。
-
7.发明申请Methods of Affecting Biological Function Through Circadian Clock Feedback Cycle by NAMPT-Mediated NAD+ Biosynthesis 审中-公开通过NAMPT介导的NAD +生物合成影响昼夜节律反馈周期的生物学功能的方法公开(公告)号：US20110123510A1
公开(公告)日：2011-05-26
申请号：US12890035
申请日：2010-09-24
申请人： Shin-ichiro Imai , Joseph Bass
发明人： Shin-ichiro Imai , Joseph Bass
IPC分类号： A61K38/45 , A61K31/455 , A61K31/706 , A61K31/7084 , A61P21/00
CPC分类号： A61K31/455 , A61K31/706 , A61K31/7084 , A61K38/45
摘要： The present invention relates to methods of regulating biological functions in a mammal that are mediated, at least in part, by the circadian clock.
摘要翻译： 本发明涉及调节至少部分由昼夜节律钟介导的哺乳动物的生物功能的方法。
-
8.发明申请METHODS FOR IDENTIFYING AGENTS WHICH ALTER HISTONE PROTEIN ACETYLATION, DECREASE AGING OR INCREASE LIFESPAN 有权用于鉴定肝细胞蛋白质乙酰化，降解衰老或增加生殖激素的药剂的方法公开(公告)号：US20090155834A1
公开(公告)日：2009-06-18
申请号：US12209847
申请日：2008-09-12
CPC分类号： G01N33/66 , A61K38/1709 , C12Q1/48 , G01N2500/00 , Y02A50/473 , A61K2300/00
摘要： Methods of identifying agents which alter the NAD-dependent acetylation status and mono-ADP-ribosylation of nuclear proteins are disclosed. The methods further include identifying agents which alter the life span or aging of a cell or an organism by determining the level of NAD-dependent acetylation and/or ADP ribosylation of a nuclear protein. The invention also relates to a mammalian Sir2 protein which acetylates or deacetylates nuclear proteins in a NAD-dependent manner and has mono-ADP-ribosyltransferase activity. Host cells producing the Sir2 protein and antibodies to the Sir2 protein are also provided.
摘要翻译： 公开了鉴定改变核蛋白的NAD依赖性乙酰化状态和单ADP-核糖基化的试剂的方法。 所述方法还包括通过确定核蛋白的NAD依赖性乙酰化和/或ADP核糖基化的水平来鉴定改变细胞或生物体的寿命或衰老的试剂。 本发明还涉及哺乳动物Sir2蛋白，其以NAD依赖性方式乙酰化或脱乙酰化核蛋白并具有单ADP-核糖基转移酶活性。 还提供了产生Sir2蛋白的宿主细胞和Sir2蛋白的抗体。
-
9.发明授权公开(公告)号：US08652797B2
公开(公告)日：2014-02-18
申请号：US12209847
申请日：2008-09-12
CPC分类号： G01N33/66 , A61K38/1709 , C12Q1/48 , G01N2500/00 , Y02A50/473 , A61K2300/00
摘要： Methods of NAD-dependent of at least one lysine residue in an acetylated protein are disclosed. The methods include combining the acetylated protein with an isolated Sir2 protein or fragment that includes a core domain of the Sir2 protein. The Sir2 protein or fragment of the Sir2 protein can include a human Sir2 protein or a fragment of a human Sir2 protein.
摘要翻译： 公开了乙酰化蛋白中至少一个赖氨酸残基的NAD依赖性的方法。 所述方法包括将乙酰化蛋白与包含Sir2蛋白的核心结构域的分离的Sir2蛋白或片段组合。 Sir2蛋白或Sir2蛋白的片段可以包括人Sir2蛋白或人Sir2蛋白的片段。
-
公开(公告)号：US20130071378A1
公开(公告)日：2013-03-21
申请号：US13523412
申请日：2012-06-14
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/44 , G01N2333/4703 , G01N2333/916 , G01N2500/02
摘要： Methods for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound are described.
摘要翻译： 描述了通过向化合物提供包含转录因子Sir2和Sir2辅因子的测试混合物并在化合物存在下评价测试混合物的组分的活性来筛选化合物的方法。
-
-
-
-
-
-
-
-
-
搜索结果
14 条记录 (耗时 0.026 秒)
