公开(公告)号：US08361305B2

公开(公告)日：2013-01-29

申请号：US12840590

申请日：2010-07-21

申请人： Junko Ikeda ,  Kunihiko Machida ,  Shinki Kojima

发明人： Junko Ikeda ,  Kunihiko Machida ,  Shinki Kojima

IPC分类号： G01N27/327

CPC分类号： G01N33/92

摘要： To provide a method of analyzing a lipid component in serum which comprises: a first total acid concentration measurement step of electrochemically measuring the total acid concentration in a serum-containing analyte solution in the presence of a water-soluble quinone derivative; a fatty acid production step of allowing a lipid component in the serum to react with one or more kinds of enzymes which specifically act on the lipid component to produce a fatty acid; a second total acid concentration measurement step of electrochemically measuring the total acid concentration in the serum-containing analyte solution in the presence of the water-soluble quinone derivative; and a lipid component concentration calculation step of calculating the serum concentration of the lipid component based on the total acid concentration measured in the first total acid concentration measurement step and the total acid concentration measured in the second total acid measurement step.

摘要翻译： 提供分析血清中的脂质成分的方法，该方法包括：在水溶性醌衍生物的存在下电化学测定含血清分析物溶液中的总酸浓度的第一总酸浓度测定步骤; 使脂质组分在血清中与一种或多种特异性作用于脂质成分以产生脂肪酸的酶反应的脂肪酸生产步骤; 在水溶性醌衍生物的存在下电化学测定含有血清的分析物溶液中的总酸浓度的第二总酸浓度测定步骤; 以及脂质成分浓度计算步骤，基于在第一总酸浓度测定步骤中测定的总酸浓度和在第二全酸测定步骤中测定的总酸浓度，算出脂质成分的血清浓度。

公开(公告)号：US20100213081A1

公开(公告)日：2010-08-26

申请号：US12679339

申请日：2008-10-10

申请人： Nobuhiko Ozaki ,  Shinki Kojima ,  Tetsuo Yukimasa

发明人： Nobuhiko Ozaki ,  Shinki Kojima ,  Tetsuo Yukimasa

IPC分类号： G01N27/26 ,  G01N33/487

CPC分类号： G01N33/5438

摘要： This invention provides an apparatus for determining a biologically specifically reactive substance using the acceleration of a biologically specific reaction by pearl chaining of carrier particles. The apparatus can suppress the generation of heat caused by the pearl chaining to suppress the vaporization of a sample solution. The apparatus comprises a substrate comprising a pair of electrodes and a reaction field disposed between the pair of electrodes, voltage applying means for applying an alternating voltage to the pair of electrodes, and carrier particles which are disposed in the reaction field and undergo pearl chaining upon exposure of an alternating electric field applied to the reaction field by the alternating voltage. The apparatus is characterized in that the voltage applying means applies two or more alternating voltages different from each other in amplitude.

摘要翻译： 本发明提供一种用于通过载体颗粒的珍珠链连接加速生物特异性反应来确定生物特异性反应物质的装置。 该装置可以抑制由珍珠链引起的热量的产生，以抑制样品溶液的蒸发。 该装置包括：基板，包括一对电极和设置在该对电极之间的反应场;施加电压到一对电极的电压施加装置;以及布置在反应场中并承载珍珠链的载体颗粒 通过交流电压施加到反应场的交变电场的暴露。 该装置的特征在于，电压施加装置在振幅上施加彼此不同的两个或更多个交流电压。

公开(公告)号：US20100096276A1

公开(公告)日：2010-04-22

申请号：US12444077

申请日：2007-10-01

申请人： Shinki Kojima ,  Tomohiro Yamamoto ,  Fumihisa Kitawaki ,  Tetsuo Yukimasa

发明人： Shinki Kojima ,  Tomohiro Yamamoto ,  Fumihisa Kitawaki ,  Tetsuo Yukimasa

IPC分类号： G01N27/26

CPC分类号： G01N27/3272 ,  C12Q1/005 ,  C12Q1/006

摘要： A multicomponent analysis sensor for measuring two or more kinds of the subjects to be measured by using redox reactions, which is a multicomponent analysis sensor comprising a liquid sample inlet from which a liquid sample containing two or more kinds of the subjects to be measured is introduced, a first measurement chamber, a second measurement chamber, a first channel connecting the above-described liquid sample inlet to the above-described first measurement chamber and a second channel connecting the above-described first measurement chamber to the second measurement chamber, wherein the above-described first measurement chamber and the above-described second measurement chamber respectively have a working electrode and a counter electrode. A first reagent layer containing an enzyme and an electron transfer substance is provided in the first channel or the first chamber, while another reagent layer containing an enzyme is provided in the second channel or the second chamber.

摘要翻译： 一种多组分分析传感器，用于通过使用氧化还原反应来测量要测量的两种或更多种待测对象，所述氧化还原反应是包含液体样品入口的多组分分析传感器，其中引入含有两种或更多种待测对象的液体样品 第一测量室，第二测量室，将上述液体样品入口连接到上述第一测量室的第一通道和将上述第一测量室连接到第二测量室的第二通道，其中， 上述第一测量室和上述第二测量室分别具有工作电极和对电极。 在第一通道或第一室中设置含有酶和电子转移物质的第一试剂层，而在第二通道或第二室中设置含有酶的试剂层。

公开(公告)号：US08329116B2

公开(公告)日：2012-12-11

申请号：US12741435

申请日：2008-11-21

申请人： Shuhei Tanaka ,  Tomohiro Yamamoto ,  Nobuhiko Ozaki ,  Shinki Kojima

发明人： Shuhei Tanaka ,  Tomohiro Yamamoto ,  Nobuhiko Ozaki ,  Shinki Kojima

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC分类号： G01N33/54366 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0415 ,  Y10T436/11

摘要： Provided is a measuring chip, which can spot a specimen liquid of a minute quantity for an immune agglutination easily and precisely to the measuring chip and which can prevent the flow of the specimen liquid and the contamination of the specimen. The measuring chip comprises at least two substrates, a spacer arranged between the substrates, at least one passage made of the substrates and the spacer into a hollow shape and having two open ends, counter electrodes for applying an electric field, and a specimen-spotted portion to be spotted with the specimen. The specimen-spotted portion has a structure made of a square, circular, elliptical or sector shape by that spacer. Moreover, the specimen-spotted portion has two open ends, one of which is connected to one open end of that passage.

摘要翻译： 提供了一种测量芯片，其可以容易且准确地将测量芯片上的微量的微量样品液体点样，并且可以防止样品液体的流动和样品的污染。 测量芯片包括至少两个基板，布置在基板之间的间隔件，由基板和间隔件形成中空形状的至少一个通道，并具有两个开口端，用于施加电场的对电极和被检测点 用样品点样的部分。 样品点状部分具有由该间隔物形成的正方形，圆形，椭圆形或扇形的结构。 此外，样本点状部分具有两个开口端，其中一个连接到该通道的一个开口端。

公开(公告)号：US20110024306A1

公开(公告)日：2011-02-03

申请号：US12840590

申请日：2010-07-21

申请人： Junko IKEDA ,  Kunihiko MACHIDA ,  Shinki KOJIMA

发明人： Junko IKEDA ,  Kunihiko MACHIDA ,  Shinki KOJIMA

IPC分类号： G01N27/26 ,  G01N33/50

CPC分类号： G01N33/92

摘要： To provide a method of analyzing a lipid component in serum which comprises: a first total acid concentration measurement step of electrochemically measuring the total acid concentration in a serum-containing analyte solution in the presence of a water-soluble quinone derivative; a fatty acid production step of allowing a lipid component in the serum to react with one or more kinds of enzymes which specifically act on the lipid component to produce a fatty acid; a second total acid concentration measurement step of electrochemically measuring the total acid concentration in the serum-containing analyte solution in the presence of the water-soluble quinone derivative; and a lipid component concentration calculation step of calculating the serum concentration of the lipid component based on the total acid concentration measured in the first total acid concentration measurement step and the total acid concentration measured in the second total acid measurement step.

摘要翻译： 提供分析血清中的脂质成分的方法，该方法包括：在水溶性醌衍生物的存在下电化学测定含血清分析物溶液中的总酸浓度的第一总酸浓度测定步骤; 使脂质组分在血清中与一种或多种特异性作用于脂质成分以产生脂肪酸的酶反应的脂肪酸生产步骤; 在水溶性醌衍生物的存在下电化学测定含有血清的分析物溶液中的总酸浓度的第二总酸浓度测定步骤; 以及脂质成分浓度计算步骤，基于在第一总酸浓度测定步骤中测定的总酸浓度和在第二全酸测定步骤中测定的总酸浓度，算出脂质成分的血清浓度。

公开(公告)号：US20100266452A1

公开(公告)日：2010-10-21

申请号：US12741435

申请日：2008-11-21

申请人： Shuhei Tanaka ,  Tomohiro Yamamoto ,  Nobuhiko Ozaki ,  Shinki Kojima

发明人： Shuhei Tanaka ,  Tomohiro Yamamoto ,  Nobuhiko Ozaki ,  Shinki Kojima

IPC分类号： G01N33/544

CPC分类号： G01N33/54366 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0415 ,  Y10T436/11

摘要： Provided is a measuring chip, which can spot a specimen liquid of a minute quantity for an immune agglutination easily and precisely to the measuring chip and which can prevent the flow of the specimen liquid and the contamination of the specimen. The measuring chip comprises at least two substrates, a spacer arranged between the substrates, at least one passage made of the substrates and the spacer into a hollow shape and having two open ends, counter electrodes for applying an electric field, and a specimen-spotted portion to be spotted with the specimen. The specimen-spotted portion has a structure made of a square, circular, elliptical or sector shape by that spacer. Moreover, the specimen-spotted portion has two open ends, one of which is connected to one open end of that passage.

摘要翻译： 提供了一种测量芯片，其可以容易且准确地将测量芯片上的微量的微量样品液体点样，并且可以防止样品液体的流动和样品的污染。 测量芯片包括至少两个基板，布置在基板之间的间隔件，由基板和间隔件形成中空形状的至少一个通道，并具有两个开口端，用于施加电场的对电极和被检测点 用样品点样的部分。 样品点状部分具有由该间隔物形成的正方形，圆形，椭圆形或扇形的结构。 此外，样本点状部分具有两个开口端，其中一个连接到该通道的一个开口端。