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    MULTICOMPONENT ANALYSIS SENSOR AND METHOD OF MEASURING MULTIPLE COMPONENTS
    1.
    发明申请
    MULTICOMPONENT ANALYSIS SENSOR AND METHOD OF MEASURING MULTIPLE COMPONENTS 审中-公开
    多功能分析传感器和测量多个组件的方法

    公开(公告)号：US20100096276A1

    公开(公告)日：2010-04-22

    申请号：US12444077

    申请日：2007-10-01

    申请人： Shinki Kojima ,  Tomohiro Yamamoto ,  Fumihisa Kitawaki ,  Tetsuo Yukimasa

    发明人： Shinki Kojima ,  Tomohiro Yamamoto ,  Fumihisa Kitawaki ,  Tetsuo Yukimasa

    IPC分类号： G01N27/26

    CPC分类号： G01N27/3272 ,  C12Q1/005 ,  C12Q1/006

    摘要： A multicomponent analysis sensor for measuring two or more kinds of the subjects to be measured by using redox reactions, which is a multicomponent analysis sensor comprising a liquid sample inlet from which a liquid sample containing two or more kinds of the subjects to be measured is introduced, a first measurement chamber, a second measurement chamber, a first channel connecting the above-described liquid sample inlet to the above-described first measurement chamber and a second channel connecting the above-described first measurement chamber to the second measurement chamber, wherein the above-described first measurement chamber and the above-described second measurement chamber respectively have a working electrode and a counter electrode. A first reagent layer containing an enzyme and an electron transfer substance is provided in the first channel or the first chamber, while another reagent layer containing an enzyme is provided in the second channel or the second chamber.

    摘要翻译： 一种多组分分析传感器，用于通过使用氧化还原反应来测量要测量的两种或更多种待测对象，所述氧化还原反应是包含液体样品入口的多组分分析传感器，其中引入含有两种或更多种待测对象的液体样品 第一测量室，第二测量室，将上述液体样品入口连接到上述第一测量室的第一通道和将上述第一测量室连接到第二测量室的第二通道，其中， 上述第一测量室和上述第二测量室分别具有工作电极和对电极。 在第一通道或第一室中设置含有酶和电子转移物质的第一试剂层，而在第二通道或第二室中设置含有酶的试剂层。

    APPARATUS AND METHOD FOR DETECTING PRESENCE OR DETERMINING BIOLOGICALLY SPECIFICALLY REACTIVE SUBSTANCE
    3.
    发明申请
    APPARATUS AND METHOD FOR DETECTING PRESENCE OR DETERMINING BIOLOGICALLY SPECIFICALLY REACTIVE SUBSTANCE 审中-公开
    检测存在或确定生物特异性反应物质的装置和方法

    公开(公告)号：US20100213081A1

    公开(公告)日：2010-08-26

    申请号：US12679339

    申请日：2008-10-10

    申请人： Nobuhiko Ozaki ,  Shinki Kojima ,  Tetsuo Yukimasa

    发明人： Nobuhiko Ozaki ,  Shinki Kojima ,  Tetsuo Yukimasa

    IPC分类号： G01N27/26 ,  G01N33/487

    CPC分类号： G01N33/5438

    摘要： This invention provides an apparatus for determining a biologically specifically reactive substance using the acceleration of a biologically specific reaction by pearl chaining of carrier particles. The apparatus can suppress the generation of heat caused by the pearl chaining to suppress the vaporization of a sample solution. The apparatus comprises a substrate comprising a pair of electrodes and a reaction field disposed between the pair of electrodes, voltage applying means for applying an alternating voltage to the pair of electrodes, and carrier particles which are disposed in the reaction field and undergo pearl chaining upon exposure of an alternating electric field applied to the reaction field by the alternating voltage. The apparatus is characterized in that the voltage applying means applies two or more alternating voltages different from each other in amplitude.

    摘要翻译： 本发明提供一种用于通过载体颗粒的珍珠链连接加速生物特异性反应来确定生物特异性反应物质的装置。 该装置可以抑制由珍珠链引起的热量的产生，以抑制样品溶液的蒸发。 该装置包括：基板，包括一对电极和设置在该对电极之间的反应场;施加电压到一对电极的电压施加装置;以及布置在反应场中并承载珍珠链的载体颗粒 通过交流电压施加到反应场的交变电场的暴露。 该装置的特征在于，电压施加装置在振幅上施加彼此不同的两个或更多个交流电压。

    Polymerase chain reaction kit and method of manufacturing the same
    4.
    发明授权
    Polymerase chain reaction kit and method of manufacturing the same 有权
    聚合酶链反应试剂盒及其制备方法

    公开(公告)号：US07790441B2

    公开(公告)日：2010-09-07

    申请号：US12149147

    申请日：2008-04-28

    申请人： Masaya Nakatani ,  Tetsuo Yukimasa

    发明人： Masaya Nakatani ,  Tetsuo Yukimasa

    IPC分类号： C12M1/00

    CPC分类号： C07H21/00 ,  B01L3/502707 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/185

    摘要： The present invention provides a kit employed for polymerase chain reaction. The kit has a cavity and a flow channel on a substrate. The flow channel is separated from the cavity by at least a barrier formed along the cavity. Such a structure allows the cavity to be filled with a sample solution even in minute quantities. At the same time, the structure can provide a sample solution with a rapid temperature-control. The structure can therefore contribute to accelerated polymerase chain reaction.

    摘要翻译： 本发明提供了用于聚合酶链反应的试剂盒。 该套件在衬底上具有空腔和流动通道。 流动通道至少沿着空腔形成的屏障与空腔分离。 这种结构允许空腔即使以微量数量填充样品溶液。 同时，该结构可以提供具有快速温度控制的样品溶液。 因此，该结构可能有助于加速聚合酶链反应。

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    5.
    发明授权
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
    DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物

    公开(公告)号：US07297499B2

    公开(公告)日：2007-11-20

    申请号：US11489582

    申请日：2006-07-20

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is T.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T; the third base from the 3′ end to be any one of T, G or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开。根据本发明，3'端基被设计为对应于SNP的基座; 从3'端的第二个基地是T; 3'末端的第3个碱基为T，G或C任一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

    Pyrophosphate acid detection sensor, method of detection of nucleic acid, and method of discrimination of base type
    7.
    发明申请
    Pyrophosphate acid detection sensor, method of detection of nucleic acid, and method of discrimination of base type 审中-公开
    焦磷酸检测传感器，核酸检测方法，碱基鉴别方法

    公开(公告)号：US20060269915A1

    公开(公告)日：2006-11-30

    申请号：US10536014

    申请日：2004-07-02

    申请人： Tetsuo Yukimasa

    发明人： Tetsuo Yukimasa

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/42 ,  G01N27/414

    摘要： The present invention is a pyrophosphate detection sensor for detecting pyrophosphate in a sample solution, and the sensor is constituted to have a sample solution receptive part that receives the sample solution, a H+ impermeable membrane having H+-pyrophosphatase, an immobilizing layer for immobilizing the H+ impermeable membrane, and a measurement means for measuring an electrochemical alteration with the change in hydrogen ion concentration of the immobilizing layer. The H+-pyrophosphatase is provided such that it hydrolyzes pyrophosphate in the sample solution to result in change in the hydrogen ion concentration of the immobilizing layer.

    摘要翻译： 本发明是一种用于检测样品溶液中焦磷酸盐的焦磷酸盐检测传感器，该传感器构成为具有容纳样品溶液的样品溶液接收部分，具有H - >磷酸酶，用于固定不透水膜的固定层，以及用于通过固定层的氢离子浓度的变化来测量电化学改变的测量装置。 提供了H + - 磷酸酶，使得其在样品溶液中水解焦磷酸盐，导致固定化层的氢离子浓度变化。

    Method of moving fluid in capillary chip
    8.
    发明授权
    Method of moving fluid in capillary chip 有权
    在毛细管芯片中移动流体的方法

    公开(公告)号：US07055540B2

    公开(公告)日：2006-06-06

    申请号：US11195649

    申请日：2005-08-03

    申请人： Hiroaki Oka ,  Tetsuo Yukimasa ,  Maki Katagiri

    发明人： Hiroaki Oka ,  Tetsuo Yukimasa ,  Maki Katagiri

    IPC分类号： E03B1/00 ,  F15C1/00

    CPC分类号： G01N27/44791 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0661 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0036 ,  F16K2099/0074 ,  F16K2099/0084 ,  Y10T137/0318 ,  Y10T137/206 ,  Y10T137/2196 ,  Y10T137/2224

    摘要： The present invention is a method of moving a fluid in a capillary using a capillary chip having a layer including a polymer composition and a capillary formed on the surface or inside of the aforementioned layer including a polymer composition, wherein the aforementioned capillary has a movement control part, and the aforementioned movement control part includes multiple and sequential opening/closing parts; the aforementioned opening/closing part blocks movement of the fluid that flows in the aforementioned capillary by increase in the volume of the aforementioned polymer composition to result in the closed state, while it permits movement of the fluid that flows in the aforementioned capillary by decrease in the volume of the aforementioned polymer composition to result in the open state; and the aforementioned method of moving the fluid includes a step (a) of switching the aforementioned multiple opening/closing parts from the open state to the closed state sequentially in a movement direction by changing the temperature of the aforementioned polymer composition in the aforementioned movement control part, thereby moving the fluid in the aforementioned capillary.

    摘要翻译： 本发明是一种使用毛细管芯片的毛细管芯片移动流体的方法，所述毛细管芯片具有包含聚合物组合物和形成在包括聚合物组合物的上述层的表面或内部的毛细管的毛细管，其中上述毛细管具有移动控制 上述移动控制部分包括多个和顺序的打开/关闭部分; 上述打开/关闭部分通过增加上述聚合物组合物的体积来阻止在上述毛细管中流动的流体的运动，导致关闭状态，同时允许通过减少在上述毛细管中流动的流体的运动 上述聚合物组合物的体积导致打开状态; 并且上述移动流体的方法包括以下步骤：（a）通过在上述移动控制中改变上述聚合物组合物的温度，将上述多个打开/关闭部分从打开状态顺序地切换到关闭状态 从而使上述毛细管中的流体移动。

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    9.
    发明授权
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
    DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物

    公开(公告)号：US07396648B2

    公开(公告)日：2008-07-08

    申请号：US11607907

    申请日：2006-12-04

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  Y10T436/143333

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of T or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 一个基座间隔开的是G.根据本发明，3'端基被设计为对应于SNP的基极; 从3'端的第二个基地是T或G; 从3'的第三个基地是T或G的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。