公开(公告)号：US20120174256A1

公开(公告)日：2012-07-05

申请号：US13391069

申请日：2010-08-19

申请人： Ko Kato ,  Shigehiko Kanaya ,  Hideyuki Matsuura ,  Shinya Takenami ,  Tatsuya Nosho ,  Yuhki Kubo ,  Kiyotaka Ueda

发明人： Ko Kato ,  Shigehiko Kanaya ,  Hideyuki Matsuura ,  Shinya Takenami ,  Tatsuya Nosho ,  Yuhki Kubo ,  Kiyotaka Ueda

IPC分类号： A01H5/00 ,  C12N15/82 ,  G06F17/10 ,  A01H1/06 ,  C07H1/00 ,  C07H21/02 ,  C12N15/11 ,  C12P21/00

CPC分类号： C12N15/8273 ,  C12N15/113 ,  C12N15/67 ,  C12N15/8216

摘要： An object of the present invention is to identify sequence features of the 5′ UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5′ UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector:(a) 5′ UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5′ end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and (b) 5′ UTR in which one or more bases in the base sequence of the 5′ UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.

摘要翻译： 本发明的一个目的是鉴定参与环境胁迫下植物的翻译状态变化的5'UTR的序列特征，并提供重组DNA分子，表达载体，转化体等 具有上述序列特征。 本发明提供了编码含有下述定义的5'UTR（a）或（b）的mRNA的重组DNA分子，通过将重组DNA分子连接到启动子的转录起始点的紧邻位点而获得的载体， 包含载体的转化体：（a）5'UTR，其中5'端的碱基1至7的序列和碱基12至32的序列分别是碱基序列1至7和碱基序列12 至32，SEQ ID NO：4,6,20,36或60; 和（b）其中（a）的5'UTR的碱基序列中的一个或多个碱基被替换的5'UTR，并且其逃避由至少一种环境应力诱导的平移抑制，所述环境应力选自热应激和 盐胁迫

公开(公告)号：US09163254B2

公开(公告)日：2015-10-20

申请号：US13391069

申请日：2010-08-19

申请人： Ko Kato ,  Shigehiko Kanaya ,  Hideyuki Matsuura ,  Shinya Takenami ,  Tatsuya Nosho ,  Yuhki Kubo ,  Kiyotaka Ueda

发明人： Ko Kato ,  Shigehiko Kanaya ,  Hideyuki Matsuura ,  Shinya Takenami ,  Tatsuya Nosho ,  Yuhki Kubo ,  Kiyotaka Ueda

IPC分类号： C12N15/82 ,  C12N15/67 ,  C12N15/113

CPC分类号： C12N15/8273 ,  C12N15/113 ,  C12N15/67 ,  C12N15/8216

摘要： An object of the present invention is to identify sequence features of the 5′ UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5′ UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector:(a) 5′ UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5′ end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and(b) 5′ UTR in which one or more bases in the base sequence of the 5′ UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.

摘要翻译： 本发明的一个目的是鉴定参与环境胁迫下植物的翻译状态变化的5'UTR的序列特征，并提供重组DNA分子，表达载体，转化体等 具有上述序列特征。 本发明提供了编码含有下述定义的5'UTR（a）或（b）的mRNA的重组DNA分子，通过将重组DNA分子连接到启动子的转录起始点的紧邻位点而获得的载体， 包含载体的转化体：（a）5'UTR，其中5'端的碱基1至7的序列和碱基12至32的序列分别是碱基序列1至7和碱基序列12 至32，SEQ ID NO：4,6,20,36或60; 和（b）其中（a）的5'UTR的碱基序列中的一个或多个碱基被替换的5'UTR，并且其逃避由至少一种环境应力诱导的平移抑制，所述环境应力选自热应激和 盐胁迫