摘要:
An object of the present invention is to identify sequence features of the 5′ UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5′ UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector:(a) 5′ UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5′ end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and(b) 5′ UTR in which one or more bases in the base sequence of the 5′ UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.
摘要翻译:本发明的一个目的是鉴定参与环境胁迫下植物的翻译状态变化的5'UTR的序列特征,并提供重组DNA分子,表达载体,转化体等 具有上述序列特征。 本发明提供了编码含有下述定义的5'UTR(a)或(b)的mRNA的重组DNA分子,通过将重组DNA分子连接到启动子的转录起始点的紧邻位点而获得的载体, 包含载体的转化体:(a)5'UTR,其中5'端的碱基1至7的序列和碱基12至32的序列分别是碱基序列1至7和碱基序列12 至32,SEQ ID NO:4,6,20,36或60; 和(b)其中(a)的5'UTR的碱基序列中的一个或多个碱基被替换的5'UTR,并且其逃避由至少一种环境应力诱导的平移抑制,所述环境应力选自热应激和 盐胁迫
摘要:
An object of the present invention is to identify sequence features of the 5′ UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5′ UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector:(a) 5′ UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5′ end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and (b) 5′ UTR in which one or more bases in the base sequence of the 5′ UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.
摘要翻译:本发明的一个目的是鉴定参与环境胁迫下植物的翻译状态变化的5'UTR的序列特征,并提供重组DNA分子,表达载体,转化体等 具有上述序列特征。 本发明提供了编码含有下述定义的5'UTR(a)或(b)的mRNA的重组DNA分子,通过将重组DNA分子连接到启动子的转录起始点的紧邻位点而获得的载体, 包含载体的转化体:(a)5'UTR,其中5'端的碱基1至7的序列和碱基12至32的序列分别是碱基序列1至7和碱基序列12 至32,SEQ ID NO:4,6,20,36或60; 和(b)其中(a)的5'UTR的碱基序列中的一个或多个碱基被替换的5'UTR,并且其逃避由至少一种环境应力诱导的平移抑制,所述环境应力选自热应激和 盐胁迫
摘要:
The present invention relates to a static magnetic field applying structure for use in an atomic oscillator in which plural sets of magnetic field generating means are provided with spacing interposed among them, and a resonance cell is disposed in a space between the magnetic field generating means. The object of the present invention is to realize the static magnetic field applying structure which can be small-sized and produced at a low cost.
摘要:
An atomic oscillator includes a light source, a first coil initiating the light source to emit light, a resonance cell having enclosed atoms absorbing light from the light source, a second coil adjusting the resonant frequency of the atoms in the resonance cell, a resonator supplying the microwave of a predetermined frequency to the resonance cell, a control circuit generating a control voltage corresponding to a light absorption amount in the resonance cell according to the microwave frequency, and an oscillator having an output signal frequency controlled to the resonant frequency by the control voltage, wherein the first and second coils and the resonator are formed of a conductor pattern on a rigid-flexible substrate having a rigid portion and a flexible portion, and the flexible portion is wound on the periphery of the light source and the resonance cell, and connected to a connector disposed on the rigid portion.
摘要:
A compact atomic oscillator with improved frequency stability. A voltage-controlled oscillator generates an oscillation signal based on a given control voltage, and a modulator modulates it with a low-frequency signal. A phase-locked loop (PLL) upconverts the modulated oscillation signal directly to a first frequency in atomic resonance frequency band. The first frequency is an integer multiple of the oscillation signal. A frequency synthesizer produces a second frequency specified by a frequency setting unit, and a mixer combines the first and second frequencies to produce an RF signal for driving an atomic resonator. The amount of discharge lamp light passing through the atomic resonator depends on the difference between RF signal frequency and atomic resonance frequency. This quantity is measured as a resonance detection signal, and a frequency controller applies it to synchronous detection to produce a control voltage for the voltage-control oscillator.
摘要:
A rubidium atom oscillator is not influenced by a circumference noise or the like, and is excellent in the short-term stability and the phase noise characteristic. A crystal oscillator oscillates a fixed frequency as an atomic resonance frequency. A direct digital synthesizer inputs an output of the crystal oscillator as a system clock and also inputs tuned data corresponding to an error signal generated according to a resonance frequency so as to carry out a variable control of an output frequency. A frequency synthesizer synthesizes and multiplies an output of the direct digital synthesizer and applies a phase modulation with a low-frequency signal. An atomic resonator inputs an output of the frequency synthesizer and detects an error signal with respect to a resonance frequency of rubidium atoms. A tuned-data generating circuit inputs the error signal from the atomic resonator so as to generate the tuned data corresponding to the error signal. The output frequency of the direct digital synthesizer is output from the rubidium atom oscillator.
摘要:
The present invention relates to a static magnetic field applying structure for use in an atomic oscillator in which plural sets of magnetic field generating means are provided with spacing interposed among them, and a resonance cell is disposed in a space between the magnetic field generating means. The object of the present invention is to realize the static magnetic field applying structure which can be small-sized and produced at a low cost.
摘要:
In an atomic oscillator of an optical pumping system, a slot line resonator, as a microwave resonator, is arranged in a portion where atoms are excited. The slot line resonator forms a microstrip line inputting microwaves so as to be orthogonal to a slot line with a dielectric substrate being sandwiched therebetween. A container in which the atoms are enclosed is mounted on the slot line resonator, and the slot line resonator and the container are covered with a metallic case having a pumping light passage hole and a photo element.
摘要:
An oscillator controller which optimizes key circuit parameters of an excitation circuit according to the operating condition of a discharge lamp. An excitation circuit energizes a discharge lamp to produce a light beam for pumping atoms, as part of a mechanism of atomic resonance detection. The operation of the excitation circuit is monitored by a start-up voltage monitor, which asserts a voltage monitoring signal when the excitation circuit's start-up voltage is reached. A light amount monitor receives a resonance detection signal from a light sensing device to check the amount of light before and after the discharge lamp lights up. The resultant light amount monitoring signal indicates this information. Based on the two monitoring signals, a bias voltage selector selects an appropriate bias voltage that varies circuit parameters of the excitation circuit.
摘要:
The present invention provides an agent for inhibiting bolting induction and/or floral-bud differentiation in plants and the method for inhibiting the bolting induction and/or floral differentiation of plants using the above-mentioned agent for inhibiting bolting induction and/or floral-bud differentiation in plants. Theobroxide or its analog is used as the agent for inhibiting bolting induction and/or floral-bud differentiation in plants. The agent for inhibiting bolting induction and/or floral-bud differentiation in plants is applied under a plant's bolting induction and/or floral differentiation condition, and controls the bolting induction and/or floral differentiation of the plant. In the case of long-day plants, such as spinach, wherein their bolting induction and floral differentiation occur under a long-day condition, spraying on plants is done under the corresponding long-day condition. The agent for inhibiting bolting induction and/or floral-bud differentiation in plants can be applied to plants by an application method such as foliar spray.