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公开(公告)号：US20140296494A1
公开(公告)日：2014-10-02
申请号：US13980099
申请日：2012-01-17
申请人： Julien Beuchat , Prisca Campanoni , Shunhong Dai , Claudio Facchinetti , Nicolas Lugon-Moulin , Karen Oishi , Gustavo Ramirez , Sandrine Roesti , Helene Laparra , Rosa Cabrera
发明人： Julien Beuchat , Prisca Campanoni , Shunhong Dai , Claudio Facchinetti , Nicolas Lugon-Moulin , Karen Oishi , Gustavo Ramirez , Sandrine Roesti , Helene Laparra , Rosa Cabrera
IPC分类号： C07K14/005 , C12N15/82
CPC分类号： C12N15/8257 , C12N15/8205
摘要： The present invention relates to protein expression in plants, particularly the large-scale production of recombinant polypeptides in whole Nicotiana tabacum plants. The use of preselected combination of N. tabacum varieties and Agrobacterium strains, optionally including one or more improvements to the transient expression-based methods of the invention, enables the production of large quantities of a heterologous polypeptides economically and in a short period of time.
摘要翻译： 本发明涉及植物中的蛋白质表达，特别是在整个烟草植物中大规模生产重组多肽。 使用预选的N. tabacum品种和农杆菌菌株的组合，任选地包括对本发明的基于瞬时表达的方法的一个或多个改进，使得能够在短时间内经济地和在短时间内生产大量的异源多肽。
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公开(公告)号：US20080096278A1
公开(公告)日：2008-04-24
申请号：US11893640
申请日：2007-08-17
申请人： Roger Beachy , Silvana Petruccelli , Shunhong Dai
发明人： Roger Beachy , Silvana Petruccelli , Shunhong Dai
CPC分类号： C12N15/8216 , C12N15/8223 , C12N15/8261 , Y02A40/146
摘要： A method of activating the rice tungro bacilliform virus (RTBV) promoter in vivo is disclosed. The RTBV promoter is activated by exposure to at least one protein selected from the group consisting of Rf2a and Rf2b.
摘要翻译： 公开了一种在体内激活稻瘟病毒（RTBV）启动子的方法。 通过暴露于选自Rf2a和Rf2b的至少一种蛋白质来活化RTBV启动子。
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公开(公告)号：US10472644B2
公开(公告)日：2019-11-12
申请号：US13980099
申请日：2012-01-17
申请人： Julien Beuchat , Prisca Campanoni , Shunhong Dai , Claudio Facchinetti , Nicolas Lugon-Moulin , Karen Oishi , Gustavo Ramirez , Sandrine Roesti , Helene Laparra , Rosa Cabrera
发明人： Julien Beuchat , Prisca Campanoni , Shunhong Dai , Claudio Facchinetti , Nicolas Lugon-Moulin , Karen Oishi , Gustavo Ramirez , Sandrine Roesti , Helene Laparra , Rosa Cabrera
IPC分类号： C12N15/82
摘要： The present invention relates to protein expression in plants, particularly the large-scale production of recombinant polypeptides in whole Nicotiana tabacum plants. The use of preselected combination of N. tabacum varieties and Agrobacterium strains, optionally including one or more improvements to the transient expression-based methods of the invention, enables the production of large quantities of a heterologous polypeptides economically and in a short period of time.
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4.发明授权公开(公告)号：US07517689B2
公开(公告)日：2009-04-14
申请号：US10888613
申请日：2004-07-09
IPC分类号： C12N15/82
CPC分类号： C12N15/8217
摘要： Novel chimeric plant promoter sequences are provided, together with plant gene expression cassettes comprising such sequences. Novel transcription factors are provided, together with nucleic acid sequences encoding such transcription factors and plant gene expression cassettes comprising such nucleic acid sequences. Methods for using the chimeric plant promoter sequences and novel transcription factors in regulating the expression of at least one gene of interest are provided, together with transgenic plants comprising such chimeric plant promoter sequences and novel transcription factors.
摘要翻译： 提供新的嵌合植物启动子序列以及包含这种序列的植物基因表达盒。 提供新的转录因子以及编码这种转录因子的核酸序列和包含这种核酸序列的植物基因表达盒。 提供了将嵌合植物启动子序列和新转录因子用于调节至少一种目的基因表达的方法，以及包含这种嵌合植物启动子序列和新转录因子的转基因植物。
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5.发明授权公开(公告)号：US07847064B2
公开(公告)日：2010-12-07
申请号：US11347750
申请日：2006-02-03
IPC分类号： C07K14/005
CPC分类号： C12N15/8217 , C07K14/4702
摘要： Novel chimeric plant promoter sequences are provided, together with plant gene expression cassettes comprising such sequences. In certain preferred embodiments, the chimeric plant promoters comprise the BoxII cis element and/or derivatives thereof. In addition, novel transcription factors are provided, together with nucleic acid sequences encoding such transcription factors and plant gene expression cassettes comprising such nucleic acid sequences. In certain preferred embodiments, the novel transcription factors comprise the acidic domain, or fragments thereof, of the RF2a transcription factor. Methods for using the chimeric plant promoter sequences and novel transcription factors in regulating the expression of at least one gene of interest are provided, together with transgenic plants comprising such chimeric plant promoter sequences and novel transcription factors.
摘要翻译： 提供新的嵌合植物启动子序列以及包含这种序列的植物基因表达盒。 在某些优选的实施方案中，嵌合植物启动子包含BoxII顺式元件和/或其衍生物。 此外，提供了新的转录因子以及编码这种转录因子的核酸序列和包含这种核酸序列的植物基因表达盒。 在某些优选实施方案中，新转录因子包含RF2a转录因子的酸性结构域或其片段。 提供了将嵌合植物启动子序列和新转录因子用于调节至少一种目的基因表达的方法，以及包含这种嵌合植物启动子序列和新转录因子的转基因植物。
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公开(公告)号：US07276370B2
公开(公告)日：2007-10-02
申请号：US10399699
申请日：2001-10-23
CPC分类号： C12N15/8216 , C12N15/8223 , C12N15/8261 , Y02A40/146
摘要： A method of activating the rice tungro bacilliform virus (RTBV) promoter in vivo is disclosed. The RTBV promoter is activated by exposure to at least one protein selected from the group consisting of Rf2a and Rf2b.
摘要翻译： 公开了一种在体内激活稻瘟病毒（RTBV）启动子的方法。 通过暴露于选自Rf2a和Rf2b的至少一种蛋白质来活化RTBV启动子。
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