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    Methods and compositions for regulating gene expression in plant cells
    1.
    发明授权
    Methods and compositions for regulating gene expression in plant cells 有权
    用于调节植物细胞中基因表达的方法和组合物

    公开(公告)号：US07847064B2

    公开(公告)日：2010-12-07

    申请号：US11347750

    申请日：2006-02-03

    申请人： Roger N. Beachy ,  Maria Isabel Ordiz Luis ,  Shunhong Dai

    发明人： Roger N. Beachy ,  Maria Isabel Ordiz Luis ,  Shunhong Dai

    IPC分类号： C07K14/005

    CPC分类号： C12N15/8217 ,  C07K14/4702

    摘要： Novel chimeric plant promoter sequences are provided, together with plant gene expression cassettes comprising such sequences. In certain preferred embodiments, the chimeric plant promoters comprise the BoxII cis element and/or derivatives thereof. In addition, novel transcription factors are provided, together with nucleic acid sequences encoding such transcription factors and plant gene expression cassettes comprising such nucleic acid sequences. In certain preferred embodiments, the novel transcription factors comprise the acidic domain, or fragments thereof, of the RF2a transcription factor. Methods for using the chimeric plant promoter sequences and novel transcription factors in regulating the expression of at least one gene of interest are provided, together with transgenic plants comprising such chimeric plant promoter sequences and novel transcription factors.

    摘要翻译： 提供新的嵌合植物启动子序列以及包含这种序列的植物基因表达盒。 在某些优选的实施方案中，嵌合植物启动子包含BoxII顺式元件和/或其衍生物。 此外，提供了新的转录因子以及编码这种转录因子的核酸序列和包含这种核酸序列的植物基因表达盒。 在某些优选实施方案中，新转录因子包含RF2a转录因子的酸性结构域或其片段。 提供了将嵌合植物启动子序列和新转录因子用于调节至少一种目的基因表达的方法，以及包含这种嵌合植物启动子序列和新转录因子的转基因植物。

    Methods and compositions for regulating gene expression in plant cells
    2.
    发明授权
    Methods and compositions for regulating gene expression in plant cells 失效
    用于调节植物细胞中基因表达的方法和组合物

    公开(公告)号：US07517689B2

    公开(公告)日：2009-04-14

    申请号：US10888613

    申请日：2004-07-09

    申请人： Roger N. Beachy ,  Shunhong Dai ,  Maria Isabel Ordiz Luis

    发明人： Roger N. Beachy ,  Shunhong Dai ,  Maria Isabel Ordiz Luis

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8217

    摘要： Novel chimeric plant promoter sequences are provided, together with plant gene expression cassettes comprising such sequences. Novel transcription factors are provided, together with nucleic acid sequences encoding such transcription factors and plant gene expression cassettes comprising such nucleic acid sequences. Methods for using the chimeric plant promoter sequences and novel transcription factors in regulating the expression of at least one gene of interest are provided, together with transgenic plants comprising such chimeric plant promoter sequences and novel transcription factors.

    摘要翻译： 提供新的嵌合植物启动子序列以及包含这种序列的植物基因表达盒。 提供新的转录因子以及编码这种转录因子的核酸序列和包含这种核酸序列的植物基因表达盒。 提供了将嵌合植物启动子序列和新转录因子用于调节至少一种目的基因表达的方法，以及包含这种嵌合植物启动子序列和新转录因子的转基因植物。

    Resistance in plants to infection by ssDNA virus using inoviridae virus ssDNA-binding protein, compositions and methods of use
    4.
    发明授权
    Resistance in plants to infection by ssDNA virus using inoviridae virus ssDNA-binding protein, compositions and methods of use 失效
    使用病毒载体ssDNA结合蛋白的ssDNA病毒感染植物抗性，组合物和使用方法

    公开(公告)号：US06852907B1

    公开(公告)日：2005-02-08

    申请号：US09622500

    申请日：1999-03-03

    申请人： Malla Padidam ,  Roger N. Beachy ,  Claude M. Fauquet

    发明人： Malla Padidam ,  Roger N. Beachy ,  Claude M. Fauquet

    IPC分类号： C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N15/00 ,  C12P21/06

    CPC分类号： C12N15/8283

    摘要： The invention describes methods for producing plant resistance to a ssDNA virus, particularly a geminivirus such as mastrevirus, curtovirus or begomovirus. The method comprises introducing a ssDNA-binding protein of the Inoviridae virus into the plant, and includes a phage coat protein, particularly, a coliphage gene 5 protein. The invention also describes a transgenic plant comprising a gene that expresses the ssDNA-binding protein and vectors for expressing the protein in plants.

    摘要翻译： 本发明描述了用于产生植物对ssDNA病毒，特别是诸如猛禽病毒，曲霉病毒或枯草芽孢杆菌病的双因素病毒抗性的方法。 该方法包括将野生病毒科病毒的ssDNA结合蛋白引入植物中，并且包括噬菌体外壳蛋白，特别是大肠杆菌噬菌体基因5蛋白。 本发明还描述了一种转基因植物，其包含表达ssDNA结合蛋白的基因和用于在植物中表达蛋白质的载体。

    Protection of plants against viral infection
    7.
    发明授权
    Protection of plants against viral infection 失效
    保护植物免受病毒感染

    公开(公告)号：US06608241B1

    公开(公告)日：2003-08-19

    申请号：US06917027

    申请日：1986-10-09

    申请人： Roger N. Beachy ,  Robert T. Fraley ,  Stephen G. Rogers

    发明人： Roger N. Beachy ,  Robert T. Fraley ,  Stephen G. Rogers

    IPC分类号： C12N1533

    CPC分类号： C12N15/1131 ,  A01H1/00 ,  C07K14/005 ,  C12N15/8283 ,  C12N15/87 ,  C12N2750/12022 ,  C12N2770/14022 ,  C12N2770/40022

    摘要： The invention involves recombinant, double-stranded DNA that contains a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus, a DNA sequence that causes the production of an RNA sequence encoding the coat protein of said plant virus, and a 3′ non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3′ end of said RNA sequence; which double-stranded DNA can be used in a method for genetically transforming plants to produce genetically transformed plant cells and plants that are resistant to virus infection.

    摘要翻译： 本发明涉及包含在植物细胞中起作用以产生植物病毒RNA序列的启动子的重组双链DNA，导致产生编码所述植物病毒外壳蛋白的RNA序列的DNA序列， 和3'非翻译区，其在植物细胞中起作用以引起多聚腺苷酸核苷酸加入所述RNA序列的3'末端; 该双链DNA可用于遗传转化植物以产生对病毒感染有抗性的遗传转化的植物细胞和植物的方法。

    Plant promoter
    8.
    发明授权
    Plant promoter 失效
    植物启动子

    公开(公告)号：US5824857A

    公开(公告)日：1998-10-20

    申请号：US789738

    申请日：1991-11-08

    申请人： Roger N. Beachy ,  Maitrayee Bhattacharyya

    发明人： Roger N. Beachy ,  Maitrayee Bhattacharyya

    IPC分类号： C07K14/01 ,  C12N15/05 ,  C12N15/82

    CPC分类号： C07K14/005 ,  C12N15/8223 ,  C12N2730/00022

    摘要： A genome length transcript promoter from a badnavirus, rice tungro bacilliform virus (RTBV), is disclosed and its DNA sequence provided. This promoter drives expression specifically in vascular tissues of plants. This promoter sequence may be utilized in a chimeric gene to drive the tissue specific expression of a foreign structural gene in vascular tissue of transgenic plants.

    摘要翻译： 公开了来自恶性疟原虫，稻tung杆菌病毒（RTBV）的基因组长度转录物启动子，并提供其DNA序列。 该启动子在植物的血管组织中特异性地驱动表达。 该启动子序列可用于嵌合基因以驱动转基因植物的血管组织中外源结构基因的组织特异性表达。