公开(公告)号：US09134302B2

公开(公告)日：2015-09-15

申请号：US08479995

申请日：1995-06-07

Applicant: Robert G. Pergolizzi ,  Jannis G. Stavrianopoulos ,  Elazar Rabbani ,  Dean L. Engelhardt ,  Stan Kline ,  Paula J. Olsiewski

Inventor： Robert G. Pergolizzi ,  Jannis G. Stavrianopoulos ,  Elazar Rabbani ,  Dean L. Engelhardt ,  Stan Kline ,  Paula J. Olsiewski

IPC: C07H21/00 ,  G01N33/53 ,  C12Q1/68

CPC classification number: G01N33/53 ,  C12Q1/6813 ,  C12Q1/6876 ,  Y10T436/143333

Abstract: A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing (B) a molecular bridging entity having thereon: (i) a portion capable of recognizing the molecularly recognizable portion on the analyte; and (ii) a portion comprising a polynucleotide sequence; and (C) a signalling entity having thereon: (i) a polynucleotide portion capable of annealing to the polynucleotide portion of the bridging entity, thereby to form a stable polynucleotide hybrid, and (ii) a signal generating portion; forming a complex comprising: (1) the analyte (A) complexed through its molecularly recognizable portion to (2) the recognizing portion of the entity (B); the entity (B) being complexed through the polynucleotide portion thereon to (3) the polynucleotide portion of the signalling entity; and detecting a signal by means of the signal generating portion present in the complex.

Abstract translation: 在样品中检测其上具有分子可识别部分的分析物（A）的方法，其包括：提供（B）其上具有其上的分子桥接实体：（i）能够识别分析物上的分子识别部分的部分; 和（ii）包含多核苷酸序列的部分; 和（C）其上具有的信令实体：（i）能够退火到所述桥接实体的多核苷酸部分从而形成稳定的多核苷酸杂交体的多核苷酸部分，和（ii）信号产生部分; 形成复合物，其包括：（1）通过其分子可识别部分复合的分析物（A）至（2）实体（B）的识别部分; 实体（B）通过其上的多核苷酸部分复合到（3）信令实体的多核苷酸部分; 以及通过存在于复合体中的信号产生部分来检测信号。

公开(公告)号：US20100273145A1

公开(公告)日：2010-10-28

申请号：US08479995

申请日：1995-06-07

Applicant: ROBERT PERGOLIZZI ,  JANNIS STAVRIANOPOULOS ,  ELAZAR RABBANI ,  DEAN ENGELHARDT ,  STAN KLINE ,  PAULA OLSIEWSKI

Inventor： ROBERT PERGOLIZZI ,  JANNIS STAVRIANOPOULOS ,  ELAZAR RABBANI ,  DEAN ENGELHARDT ,  STAN KLINE ,  PAULA OLSIEWSKI

IPC: C12Q1/68 ,  C12N9/96 ,  C12N7/01 ,  C12N1/21 ,  G01N33/00 ,  C07K16/00 ,  C07K14/00 ,  C07K14/42 ,  C07H21/04

CPC classification number: G01N33/53 ,  C12Q1/6813 ,  C12Q1/6876 ,  Y10T436/143333

Abstract: A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing (B) a molecular bridging entity having thereon: (i) a portion capable of recognizing the molecularly recognizable portion on the analyte; and (ii) a portion comprising a polynucleotide sequence; and (C) a signalling entity having thereon: (i) a polynucleotide portion capable of annealing to the polynucleotide portion of the bridging entity, thereby to form a stable polynucleotide hybrid, and (ii) a signal generating portion; forming a complex comprising: (1) the analyte (A) complexed through its molecularly recognizable portion to (2) the recognizing portion of the entity (B); the entity (B) being complexed through the polynucleotide portion thereon to (3) the polynucleotide portion of the signalling entity; and detecting a signal by means of the signal generating portion present in the complex.

Abstract translation: 在样品中检测其上具有分子可识别部分的分析物（A）的方法，其包括：提供（B）其上具有其上的分子桥接实体：（i）能够识别分析物上的分子识别部分的部分; 和（ii）包含多核苷酸序列的部分; 和（C）其上具有的信令实体：（i）能够退火到所述桥接实体的多核苷酸部分从而形成稳定的多核苷酸杂交体的多核苷酸部分，和（ii）信号产生部分; 形成复合物，其包括：（1）通过其分子可识别部分复合的分析物（A）至（2）实体（B）的识别部分; 实体（B）通过其上的多核苷酸部分复合到（3）信令实体的多核苷酸部分; 以及通过存在于复合体中的信号产生部分来检测信号。