公开(公告)号：US20090326359A1

公开(公告)日：2009-12-31

申请号：US12375776

申请日：2007-07-11

申请人： Bernardus Hendrikus Wilhelmus Hendriks ,  Ruth Wihelmine Ireen De Boer ,  Gerhard Spekowius ,  Stein Kuipeer ,  Nijs Cornelis Van Der Vaart

发明人： Bernardus Hendrikus Wilhelmus Hendriks ,  Ruth Wihelmine Ireen De Boer ,  Gerhard Spekowius ,  Stein Kuipeer ,  Nijs Cornelis Van Der Vaart

IPC分类号： A61B6/00

CPC分类号： G01N21/33 ,  A61B5/0066 ,  A61B5/415 ,  A61B5/417 ,  A61B5/418 ,  A61B5/6852 ,  G01N15/1475 ,  G01N21/6458

摘要： The invention relates to a method of determining in vivo in a human or animal subject the amount of nuclear DNA by first localizing cell nuclei of living tissue and subsequently measuring the nuclear UV absorbance using confocal scanning microscopy. The invention relates also to a method for detecting cancerous cells in vivo in a human or animal subject by first identifying the localization of cell nuclei in living tissue and subsequently determining the nuclear UV absorbance by laser scanning confocal microscopy. Furthermore, the invention relates to a method of diagnosing cancer in a human or animal subject in vivo relying on a combination of identifying the localization of cell nuclei in living tissue and measuring nuclear UV absorbance by laser scanning confocal microscopy.

摘要翻译： 本发明涉及一种通过首先定位活组织的细胞核并随后使用共聚焦扫描显微镜测量核紫外吸收光，在人体或动物体内测定核DNA数量的方法。 本发明还涉及通过首先识别活体组织中细胞核的定位并随后通过激光扫描共焦显微镜确定核紫外吸收光，来检测人或动物受试者体内癌细胞的方法。 此外，本发明涉及一种通过识别活体组织中细胞核的定位和通过激光扫描共焦显微镜测量核紫外吸收的组合来诊断人或动物受试者的癌症的方法。