公开(公告)号：US06734318B2

公开(公告)日：2004-05-11

申请号：US09877944

申请日：2001-06-08

申请人： Dennis P. Curran ,  Stephen G. Weber ,  Hiroyuki Nakamura ,  Bruno Linclau ,  Lifang Sun

发明人： Dennis P. Curran ,  Stephen G. Weber ,  Hiroyuki Nakamura ,  Bruno Linclau ,  Lifang Sun

IPC分类号： C07F704

CPC分类号： C07D207/333 ,  C07B37/04 ,  C07B61/00 ,  C07B63/00 ,  C07C1/325 ,  C07C17/02 ,  C07C45/46 ,  C07D307/46 ,  C07D333/22 ,  C07C15/52 ,  C07C22/04 ,  C07C23/10 ,  C07C49/84

摘要： A method of reacting a first compound to produce a second compound includes the step of contacting a first non-fluorous phase including the first compound with a first fluorous phase at a first phase interface. The first compound distributes between the first fluorous phase and the first non-fluorous phase. The method further includes the steps of contacting the first fluorous phase with a second non-fluorous phase at a second phase interface and including at least a third compound in the second non-fluorous phase that reacts with the first compound to produce the second compound. The second compound has a distribution coefficient less than the first compound. This method can, for example be used to separate the second compound from unreacted first compound wherein, for example, the first compound is of a fluorous nature and distributes more readily into (or transports more quickly through) the fluorous phase than does the second compound. In general, the fluorous phase serves as a barrier to prevent the two non-fluorous phases from mixing, but molecules that can migrate through the fluorous phase can pass from one side to the other.

摘要翻译： 使第一化合物反应以产生第二化合物的方法包括使第一化合物的第一非氟相与第一相界面处的第一氟相接触的步骤。 第一化合物在第一氟相和第一非氟相之间分配。 该方法还包括以下步骤：在第二相界面处使第一氟相与第二非氟相接触，并且在第二非氟相中包含至少第三化合物，其与第一化合物反应以产生第二化合物。 第二化合物的分配系数小于第一化合物。 该方法可以例如用于将第二化合物与未反应的第一化合物分离，其中例如第一化合物具有氟性质，并且比第二化合物更容易地分配（或更快地通过）氟相 。 通常，氟相用作阻止两个非氟相混合的屏障，但是可以迁移穿过氟相的分子可以从一侧传递到另一侧。

公开(公告)号：US4293310A

公开(公告)日：1981-10-06

申请号：US130555

申请日：1980-03-14

申请人： Stephen G. Weber

发明人： Stephen G. Weber

IPC分类号： G01N33/532 ,  G01N33/533 ,  G01N33/543 ,  G01N33/58 ,  G01N33/54 ,  G01N27/00 ,  G01N27/52

CPC分类号： G01N33/532 ,  G01N33/533 ,  G01N33/5438 ,  G01N33/58 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/816 ,  Y10S436/817

摘要： A method and apparatus for the determination of trace amounts of chemicals in a system, as for example the immunoassay determination of hormones, peptides and drugs in biological fluids, applies photoelectrochemistry to the field of immunoassay. The apparatus and method comprise a photoelectrochemically active molecule as a label in an immunoassay system of labeled analyte, antibody sensitive to analyte, quencher and an electrochemical flow cell with light means. The photoelectrochemically active molecule upon photoexcitation transfers an electron to a quencher molecule; the oxidized molecule is subsequently reduced with an electron from an electrode of the flow cell which is held at suitable potential. This electron is measured as photocurrent. The electrochemical flow cell is channeled for the flow of solution containing photoelectrochemically active species and quencher; and has at least one wall transparent to light such as a laser which is trained on the appropriate region of the cell; and further comprises means to isolate the spectral region of interest and means to distinguish photocurrent from nonphotocurrent. The amount of free labeled analyte in the system is determined by the photocurrent signal.

摘要翻译： 用于测定系统中微量化学物质的方法和装置，例如生物流体中的激素，肽和药物的免疫测定法，将免疫测定法中的光电化学应用于免疫测定领域。 该装置和方法包括在标记的分析物的免疫测定系统中的光电化学活性分子作为标记，对分析物敏感的抗体，猝灭剂和具有光的手段的电化学流动池。 光电激活分子在光激发下将电子转移到猝灭剂分子; 氧化分子随后用来自保持在合适电位的流动池的电极的电子还原。 该电子被测量为光电流。 电化学流通池被引导用于含有光电化学活性物质和猝灭剂的溶液流; 并且具有至少一个对诸如在细胞的适当区域上训练的激光的光透明的壁; 并且还包括用于隔离感兴趣的光谱区域的装置和用于区分光电流与非光电流的装置。 系统中游离标记的分析物的量由光电流信号确定。

公开(公告)号：US5459032A

公开(公告)日：1995-10-17

申请号：US188646

申请日：1994-01-28

申请人： Stephen G. Weber ,  Robert C. Elser ,  Jane N. Valenta

发明人： Stephen G. Weber ,  Robert C. Elser ,  Jane N. Valenta

IPC分类号： C12Q1/00 ,  C12Q1/34 ,  C12Q1/26 ,  C12Q1/42

CPC分类号： C12Q1/34 ,  C12Q1/00

摘要： A method and apparatus for performing an enzyme spot test without traditional instrumentation is disclosed. The method utilizes various reagents to allow the visual determination of the presence of an enzyme in a sample above a predetermined critical level. The enzyme reacts with the reagents producing a product according to a reaction which is timed by a chemical fuse. At the end of the reaction, a colored by-product is visible, the color intensity indicating that the enzyme occurs in the sample in quantities above the critical level.

摘要翻译： 公开了一种无需传统仪器进行酶斑测试的方法和装置。 该方法利用各种试剂来使样品中酶的存在可视化地高于预定临界水平。 根据通过化学保险丝定时的反应，酶与产生产物的试剂反应。 在反应结束时，着色的副产物是可见的，颜色强度表明酶在样品中以高于临界水平的量出现。