公开(公告)号：US20090029408A1

公开(公告)日：2009-01-29

申请号：US12179245

申请日：2008-07-24

申请人： Peter LaPan ,  Steven A. Haney

发明人： Peter LaPan ,  Steven A. Haney

IPC分类号： C12Q1/04

CPC分类号： G01N33/56966 ,  G01N33/57492 ,  G01N2333/91148

摘要： One aspect of the invention relates to a method of identifying cell adjacency in a coculture of cells. The method includes generating a first set of image data of the coculture of cells, the first set of image data comprising nuclear position data for each imaged cell; generating a second set of image data of at least a subset of the coculture of cells, the second set of image data comprising cell identifier data associated with a first cell type; generating a third set of image data of at least a subset of the coculture of cells, the third set of image data comprising cell identifier data associated with a second cell type; and identifying a group of cells in response to the nuclear position data, wherein each cell having the first cell type in the group is adjacent another cell in the group having the second cell type.

摘要翻译： 本发明的一个方面涉及鉴定细胞共培养中的细胞相邻性的方法。 该方法包括生成第一组图像数据，其中，第一组图像数据包括每个成像单元的核位置数据; 生成小区共同的至少一个子集的第二组图像数据，所述第二组图像数据包括与第一小区类型相关联的小区标识符数据; 生成小区共同组合的至少一个子集的第三组图像数据，所述第三组图像数据包括与第二小区类型相关联的小区标识符数据; 以及响应于所述核位置数据识别一组细胞，其中所述组中具有第一细胞类型的每个细胞与具有第二细胞类型的组中的另一个细胞相邻。

公开(公告)号：US07745164B2

公开(公告)日：2010-06-29

申请号：US11645056

申请日：2006-12-26

申请人： Wei Liu ,  Bradley A. Ozenberger ,  Leeying Wu ,  Ching-Hsiung Frederick Lo ,  Steven A. Haney ,  Hemchand Sookdeo ,  Jee Hyung Lee

发明人： Wei Liu ,  Bradley A. Ozenberger ,  Leeying Wu ,  Ching-Hsiung Frederick Lo ,  Steven A. Haney ,  Hemchand Sookdeo ,  Jee Hyung Lee

IPC分类号： C12Q1/48

CPC分类号： C12N9/1205

摘要： The invention provides isolated protein kinase polypeptides related to novel brain-localized protein kinases homologous to known homeodomain-interacting protein kinases (HIPKs), isolated nucleic acid molecules that encode these polypeptides, inhibitors, and methods related thereto. The novel polypeptides and nucleic acid molecules of the invention are termed HIPK4. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention, as well as inhibitors, activators, and antibodies capable of binding to the protein kinase polypeptides of the invention. The invention further provides uses of HIPK4 and its inhibitors, e.g., mutants of HIPK4 lacking protein kinase activity, for modulation of apoptosis, as well as the prevention and treatment of neurological disorders and cancers.

摘要翻译： 本发明提供与与已知同源结构域相互作用蛋白激酶（HIPK）同源的新型脑定位蛋白激酶相关的分离的蛋白激酶多肽，编码这些多肽的分离的核酸分子，抑制剂及其相关方法。 本发明的新型多肽和核酸分子称为HIPK4。 本发明还提供了遗传工程改造的表达载体，宿主细胞和包含本发明的新型核酸分子的转基因动物。 本发明还向本发明的核酸分子以及能够结合本发明的蛋白激酶多肽的抑制剂，激活剂和抗体提供反义和RNAi分子。 本发明还提供了HIPK4及其抑制剂（例如缺乏蛋白激酶活性的HIPK4的突变体）用于调节凋亡以及预防和治疗神经障碍和癌症的用途。