公开(公告)号：US07745164B2

公开(公告)日：2010-06-29

申请号：US11645056

申请日：2006-12-26

申请人： Wei Liu ,  Bradley A. Ozenberger ,  Leeying Wu ,  Ching-Hsiung Frederick Lo ,  Steven A. Haney ,  Hemchand Sookdeo ,  Jee Hyung Lee

发明人： Wei Liu ,  Bradley A. Ozenberger ,  Leeying Wu ,  Ching-Hsiung Frederick Lo ,  Steven A. Haney ,  Hemchand Sookdeo ,  Jee Hyung Lee

IPC分类号： C12Q1/48

CPC分类号： C12N9/1205

摘要： The invention provides isolated protein kinase polypeptides related to novel brain-localized protein kinases homologous to known homeodomain-interacting protein kinases (HIPKs), isolated nucleic acid molecules that encode these polypeptides, inhibitors, and methods related thereto. The novel polypeptides and nucleic acid molecules of the invention are termed HIPK4. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention, as well as inhibitors, activators, and antibodies capable of binding to the protein kinase polypeptides of the invention. The invention further provides uses of HIPK4 and its inhibitors, e.g., mutants of HIPK4 lacking protein kinase activity, for modulation of apoptosis, as well as the prevention and treatment of neurological disorders and cancers.

摘要翻译： 本发明提供与与已知同源结构域相互作用蛋白激酶（HIPK）同源的新型脑定位蛋白激酶相关的分离的蛋白激酶多肽，编码这些多肽的分离的核酸分子，抑制剂及其相关方法。 本发明的新型多肽和核酸分子称为HIPK4。 本发明还提供了遗传工程改造的表达载体，宿主细胞和包含本发明的新型核酸分子的转基因动物。 本发明还向本发明的核酸分子以及能够结合本发明的蛋白激酶多肽的抑制剂，激活剂和抗体提供反义和RNAi分子。 本发明还提供了HIPK4及其抑制剂（例如缺乏蛋白激酶活性的HIPK4的突变体）用于调节凋亡以及预防和治疗神经障碍和癌症的用途。

公开(公告)号：US20070113296A1

公开(公告)日：2007-05-17

申请号：US11645056

申请日：2006-12-26

申请人： Wei Liu ,  Bradley Ozenberger ,  Leeying Wu ,  Ching-Hsiung Lo ,  Steven Haney ,  Hemchand Sookdeo ,  Jee Hyung Lee

发明人： Wei Liu ,  Bradley Ozenberger ,  Leeying Wu ,  Ching-Hsiung Lo ,  Steven Haney ,  Hemchand Sookdeo ,  Jee Hyung Lee

IPC分类号： A01K67/027 ,  C12Q1/68 ,  G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C12N9/12

CPC分类号： C12N9/1205

摘要： The invention provides isolated protein kinase polypeptides related to novel brain-localized protein kinases homologous to known homeodomain-interacting protein kinases (HIPKs), and the isolated nucleic acid molecules that encode these polypeptides. The novel polypeptides and nucleic acid molecules of the invention are termed HIPK4. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides inhibitors, activators, and antibodies capable of binding to the protein kinase polypeptides of the invention, and their use in the prevention and treatment of neurological disorders.

摘要翻译： 本发明提供与与已知同源结构域相互作用的蛋白激酶（HIPK）同源的新型脑定位蛋白激酶相关的分离的蛋白激酶多肽以及编码这些多肽的分离的核酸分子。 本发明的新型多肽和核酸分子称为HIPK4。 本发明还提供了遗传工程改造的表达载体，宿主细胞和包含本发明的新型核酸分子的转基因动物。 本发明还向本发明的核酸分子提供反义和RNAi分子。 本发明还提供能够结合本发明的蛋白激酶多肽的抑制剂，活化剂和抗体及其在预防和治疗神经障碍中的用途。

公开(公告)号：US07208306B2

公开(公告)日：2007-04-24

申请号：US10691529

申请日：2003-10-24

申请人： Wei Liu ,  Leeying Wu ,  Roger Ford ,  Xiaobing Be

发明人： Wei Liu ,  Leeying Wu ,  Roger Ford ,  Xiaobing Be

IPC分类号： C12N9/16 ,  C12N15/00 ,  C12N5/00 ,  C12Q1/68 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12N9/16

摘要： This invention provides compositions, organisms and methodologies employing a novel human gene encoding a protein that has sequence homology to a consensus sequence of calcineurin-like phosphoesterase family are disclosed. The novel protein is encoded by a human gene comprising 4 exons. The human gene is localized in the 10p15 locus of human chromosome 10. The sequence similarities between the novel human protein and the consensus sequence of calcineurin-like phosphoesterases indicate that the novel human protein may function as a calcineurin-like protein phosphatase.

摘要翻译： 本发明提供组合物，生物体和方法学，其使用编码与钙调神经磷酸酯酶类家族的共有序列具有序列同源性的蛋白质的新型人基因。 该新蛋白由包含4个外显子的人基因编码。 人类基因位于人类10号染色体10p15位点。新型人类蛋白质与钙调神经磷酸酯酶的共有序列之间的序列相似性表明新型人类蛋白质可作为钙调神经磷酸酶蛋白磷酸酶起作用。

公开(公告)号：US20080153147A1

公开(公告)日：2008-06-26

申请号：US11982836

申请日：2007-11-05

申请人： Wei Liu ,  Leeying Wu ,  Huimin Chen

发明人： Wei Liu ,  Leeying Wu ,  Huimin Chen

IPC分类号： C12N9/12 ,  C12N15/11 ,  C12N15/52

CPC分类号： C12N9/1205

摘要： This invention provides compositions, organisms and methodologies employing a novel human protein kinase, MCRK1. The novel human kinase has sequence homology to rat myotonic dystrophy kinase-related Cdc42 binding kinase (MRCK) alpha. The gene encoding the novel kinase is localized in locus 11q13 of human chromosome 11. The novel protein kinase comprises multiple functional/structural domains that include a kinase domain, a pkinase_C domain, a DAG-PE binding domain, and a CNH domain. The sequence and structure similarity between the novel human protein and rat MRCK alpha indicates that the novel human protein may function as a downstream effector of Cdc42 in cytoskeleton reorganization.

摘要翻译： 本发明提供了使用新型人蛋白激酶MCRK1的组合物，生物体和方法。 新型人类激酶与大鼠肌强直性营养不良激酶相关的Cdc42结合激酶（MRCK）α具有序列同源性。 编码新型激酶的基因位于人染色体11的位点11q13中。新型蛋白激酶包含多个功能/结构域，其包括激酶结构域，pkinase_C结构域，DAG-PE结合结构域和CNH结构域。 新型人类蛋白质与大鼠MRCKα之间的序列和结构相似性表明新型人类蛋白质可能在细胞骨架重组中起到Cdc42的下游作用。

公开(公告)号：US20060294601A1

公开(公告)日：2006-12-28

申请号：US11505718

申请日：2006-08-17

申请人： Wei Liu ,  Leeying Wu

发明人： Wei Liu ,  Leeying Wu

IPC分类号： A01K67/027 ,  C12Q1/48 ,  C07H21/04 ,  C12P21/06 ,  C12N9/12

CPC分类号： C12Q1/485 ,  A01K2217/05 ,  A01K2217/075 ,  C12N9/1205 ,  G01N2500/04

摘要： This invention provides compositions, organisms and methodologies employing a novel human protein kinase, HPK3P23. The novel human kinase has sequence homology to the catalytic domains of several protein kinases. The gene encoding this novel protein kinase is localized in or near the 3p23 locus of the human chromosome 3. The sequence similarity between the novel human protein and the catalytic domain of protein kinases indicates that the novel human protein may function as a protein kinase.

摘要翻译： 本发明提供了使用新型人蛋白激酶HPK3P23的组合物，生物体和方法。 新型人类激酶与几种蛋白激酶的催化结构域具有序列同源性。 编码这种新型蛋白激酶的基因位于人染色体3的3p23位点或其附近。新型人蛋白质与蛋白激酶的催化结构域之间的序列相似性表明新型人蛋白质可能作为蛋白激酶发挥作用。

公开(公告)号：US07407792B2

公开(公告)日：2008-08-05

申请号：US11505718

申请日：2006-08-17

申请人： Wei Liu ,  Leeying Wu

发明人： Wei Liu ,  Leeying Wu

IPC分类号： C12N1/20 ,  C07H21/04

CPC分类号： C12Q1/485 ,  A01K2217/05 ,  A01K2217/075 ,  C12N9/1205 ,  G01N2500/04

摘要： This invention provides compositions, organisms and methodologies employing a novel human protein kinase, HPK3P23. The novel human kinase has sequence homology to the catalytic domains of several protein kinases. The gene encoding this novel protein kinase is localized in or near the 3p23 locus of the human chromosome 3. The sequence similarity between the novel human protein and the catalytic domain of protein kinases indicates that the novel human protein may function as a protein kinase.

摘要翻译： 本发明提供了使用新型人蛋白激酶HPK3P23的组合物，生物体和方法。 新型人类激酶与几种蛋白激酶的催化结构域具有序列同源性。 编码这种新型蛋白激酶的基因位于人染色体3的3p23位点或其附近。新型人蛋白质与蛋白激酶的催化结构域之间的序列相似性表明新型人蛋白质可能作为蛋白激酶发挥作用。

公开(公告)号：US07122361B2

公开(公告)日：2006-10-17

申请号：US10684190

申请日：2003-10-10

申请人： Wei Liu ,  Leeying Wu

发明人： Wei Liu ,  Leeying Wu

IPC分类号： C12N9/12

CPC分类号： C12Q1/485 ,  A01K2217/05 ,  A01K2217/075 ,  C12N9/1205 ,  G01N2500/04

摘要： This invention provides compositions, organisms and methodologies employing a novel human protein kinase, HPK3P23. The novel human kinase has sequence homology to the catalytic domains of several protein kinases. The gene encoding this novel protein kinase is localized in or near the 3p23 locus of the human chromosome 3. The sequence similarity between the novel human protein and the catalytic domain of protein kinases indicates that the novel human protein may function as a protein kinase.

摘要翻译： 本发明提供了使用新型人蛋白激酶HPK3P23的组合物，生物体和方法。 新型人类激酶与几种蛋白激酶的催化结构域具有序列同源性。 编码这种新型蛋白激酶的基因位于人染色体3的3p23位点或其附近。新型人蛋白质与蛋白激酶的催化结构域之间的序列相似性表明新型人蛋白质可能作为蛋白激酶发挥作用。

公开(公告)号：US20080178307A1

公开(公告)日：2008-07-24

申请号：US11787345

申请日：2007-04-16

申请人： Wei Liu ,  Leeying Wu ,  Roger Ford ,  Xiaobing Be

发明人： Wei Liu ,  Leeying Wu ,  Roger Ford ,  Xiaobing Be

IPC分类号： C12N15/55 ,  C12N5/10 ,  A01K67/027

CPC分类号： C12N9/16

摘要： This invention provides compositions, organisms and methodologies employing a novel human gene encoding a protein that has sequence homology to a consensus sequence of calcineurin-like phosphoesterase family are disclosed. The novel protein is encoded by a human gene comprising 4 exons. The human gene is localized in the 10p15 locus of human chromosome 10. The sequence similarities between the novel human protein and the consensus sequence of calcineurin-like phosphoesterases indicate that the novel human protein may function as a calcineurin-like protein phosphatase.

摘要翻译： 本发明提供组合物，生物体和方法学，其使用编码与钙调神经磷酸酯酶类家族的共有序列具有序列同源性的蛋白质的新型人基因。 该新蛋白由包含4个外显子的人基因编码。 人类基因位于人类10号染色体10p15位点。新型人类蛋白质与钙调神经磷酸酯酶的共有序列之间的序列相似性表明新型人类蛋白质可作为钙调神经磷酸酶蛋白磷酸酶起作用。

公开(公告)号：US07297525B2

公开(公告)日：2007-11-20

申请号：US10702496

申请日：2003-11-07

申请人： Wei Liu ,  Leeying Wu ,  Huimin Chen

发明人： Wei Liu ,  Leeying Wu ,  Huimin Chen

IPC分类号： C12N9/12 ,  C12N15/54

CPC分类号： C12N9/1205

摘要： This invention provides compositions, organisms and methodologies employing a novel human protein kinase, MCRK1. The novel human kinase has sequence homology to rat myotonic dystrophy kinase-related Cdc42 binding kinase (MRCK) alpha. The gene encoding the novel kinase is localized in locus 11q13 of human chromosome 11. The novel protein kinase comprises multiple functional/structural domains that include a kinase domain, a pkinase_C domain, a DAG-PE binding domain, and a CNH domain. The sequence and structure similarity between the novel human protein and rat MRCK alpha indicates that the novel human protein may function as a downstream effector of Cdc42 in cytoskeleton reorganization.

摘要翻译： 本发明提供了使用新型人蛋白激酶MCRK1的组合物，生物体和方法。 新型人类激酶与大鼠肌强直性营养不良激酶相关的Cdc42结合激酶（MRCK）α具有序列同源性。 编码新型激酶的基因位于人染色体11的位点11q13中。新型蛋白激酶包含多个功能/结构域，其包括激酶结构域，pkinase_C结构域，DAG-PE结合结构域和CNH结构域。 新型人类蛋白质与大鼠MRCKα之间的序列和结构相似性表明新型人类蛋白质可能在细胞骨架重组中起到Cdc42的下游作用。

公开(公告)号：US20050059025A1

公开(公告)日：2005-03-17

申请号：US10679366

申请日：2003-10-07

申请人： Wei Liu ,  Leeying Wu

发明人： Wei Liu ,  Leeying Wu

IPC分类号： C12N9/12 ,  C12Q1/68 ,  C07H21/04 ,  G01N33/53

CPC分类号： C12N9/1205

摘要： This invention provides compositions, organisms and methodologies employing a novel human protein kinase, MCRK2. The novel human kinase has sequence homology to rat myotonic dystrophy kinase-related Cdc42 binding kinase (MRCK) alpha. The gene encoding the novel kinase is localized in locus 1q42 of human chromosome 1. The novel protein kinase comprises multiple functional/structural domains that include a kinase domain, a pkinase_C domain, a DAG-PE binding domain, and a CNH domain. The sequence and structure similarity between the novel human protein and rat MRCK alpha indicates that the novel human protein may function as a downstream effector of Cdc42 in cytoskeleton reorganization.

摘要翻译： 本发明提供了使用新型人蛋白激酶MCRK2的组合物，生物体和方法。 新型人类激酶与大鼠肌强直性营养不良激酶相关的Cdc42结合激酶（MRCK）α具有序列同源性。 编码新型激酶的基因位于人染色体1的基因座1q42中。新的蛋白激酶包含多个功能/结构域，其包括激酶结构域，pkinase_C结构域，DAG-PE结合结构域和CNH结构域。 新型人类蛋白质与大鼠MRCKα之间的序列和结构相似性表明新型人类蛋白质可能在细胞骨架重组中起到Cdc42的下游作用。