公开(公告)号：US08765468B2

公开(公告)日：2014-07-01

申请号：US13901728

申请日：2013-05-24

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： C12N5/16 ,  C12N5/10 ,  C12N5/071

CPC分类号： A61F2/105 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323

摘要： A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels. The inventive device is easy to handle and manipulate for surgical transplant, can be made into large sheets to minimize the number of grafts required to cover a large surface area to be treated, and can be produced within the time frame to treat a burned individual requiring a skin graft.

摘要翻译： 一种能够对人体皮肤进行治疗性治疗和/或体外测试的装置及其制造方法。 该装置可用于烫伤，受伤或患病皮肤的皮肤伤口，并提供如正常未受伤皮肤的结构和功能，例如屏障功能，这是正常皮肤的确定性质。 该装置在生物相容性可生物降解的网状基质上含有培养的皮肤和表皮细胞。 从培养的皮肤装置的受体中，全部或部分细胞可以是自体的，其有利地消除了组织相容性的问题。 细胞也可以在遗传上修饰以提供一个或多个因素以促进植入的皮肤置换物的愈合，例如用于刺激血管生长的血管生成因子。 本发明的装置易于处理和操作用于手术移植，可以制成大片以最小化覆盖待治疗的大表面积所需的移植物的数量，并且可以在时间框架内生产以治疗需要 皮肤移植。

公开(公告)号：US20140287020A1

公开(公告)日：2014-09-25

申请号：US14299480

申请日：2014-06-09

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： A61F2/10 ,  A61L27/60

CPC分类号： A61F2/105 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323

摘要： A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels. The inventive device is easy to handle and manipulate for surgical transplant, can be made into large sheets to minimize the number of grafts required to cover a large surface area to be treated, and can be produced within the time frame to treat a burned individual requiring a skin graft.

摘要翻译： 一种能够对人体皮肤进行治疗性治疗和/或体外测试的装置及其制造方法。 该装置可用于烫伤，受伤或患病皮肤的皮肤伤口，并提供如正常未受伤皮肤的结构和功能，例如屏障功能，这是正常皮肤的确定性质。 该装置在生物相容性可生物降解的网状基质上含有培养的皮肤和表皮细胞。 从培养的皮肤装置的受体中，全部或部分细胞可以是自体的，其有利地消除了组织相容性的问题。 细胞也可以在遗传上修饰以提供一个或多个因素以促进植入的皮肤置换物的愈合，例如用于刺激血管生长的血管生成因子。 本发明的装置易于处理和操作用于手术移植，可以制成大片以最小化覆盖待治疗的大表面积所需的移植物的数量，并且可以在时间框架内生产以治疗需要 皮肤移植。

公开(公告)号：US5711172A

公开(公告)日：1998-01-27

申请号：US459024

申请日：1995-06-02

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： A61F2/00 ,  A61F2/10 ,  A61L27/24 ,  A61L27/38 ,  A61L27/60 ,  C12M3/00 ,  B29C39/26

CPC分类号： A61L27/3813 ,  A61F2/105 ,  A61L27/24 ,  A61L27/60 ,  A61F2310/00365

摘要： A method and apparatus for forming a permanent, composite skin replacement consisting of an epidermal component and a porous, resorbable, biosynthetic laminated dermal membrane component for use in wound repair. The dermal membrane is formed in the apparatus, which permits regulation of variables affecting membrane structure. The dermal membrane may be modified to incorporate biologically active molecules to enhance wound repair and to reduce infection when the skin replacement is applied to a wound.

摘要翻译： 一种用于形成由表皮组分和用于伤口修复的多孔，可再吸收的生物合成层压真皮膜组分组成的永久性复合皮肤置换剂的方法和装置。 在设备中形成真皮膜，其允许调节影响膜结构的变量。 皮肤膜可以被修饰以包含生物活性分子以增强伤口修复，并且当将皮肤置换施加于伤口时减少感染。

公开(公告)号：US09089417B2

公开(公告)日：2015-07-28

申请号：US14299480

申请日：2014-06-09

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： C12N5/16 ,  C12N5/10 ,  C12N5/071 ,  A61F2/10 ,  A61L27/38 ,  A61L27/60

CPC分类号： A61F2/105 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323

摘要： A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels. The inventive device is easy to handle and manipulate for surgical transplant, can be made into large sheets to minimize the number of grafts required to cover a large surface area to be treated, and can be produced within the time frame to treat a burned individual requiring a skin graft.

公开(公告)号：US08450108B2

公开(公告)日：2013-05-28

申请号：US12818302

申请日：2010-06-18

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： C12N5/071 ,  C12N5/00 ,  C12N5/07 ,  C12N5/02

CPC分类号： A61F2/105 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323

摘要： A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels. The inventive device is easy to handle and manipulate for surgical transplant, can be made into large sheets to minimize the number of grafts required to cover a large surface area to be treated, and can be produced within the time frame to treat a burned individual requiring a skin graft.

公开(公告)号：US20100254955A1

公开(公告)日：2010-10-07

申请号：US12818302

申请日：2010-06-18

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： A61K35/36 ,  C12Q1/02 ,  C12N5/071

CPC分类号： A61F2/105 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323

摘要： A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels. The inventive device is easy to handle and manipulate for surgical transplant, can be made into large sheets to minimize the number of grafts required to cover a large surface area to be treated, and can be produced within the time frame to treat a burned individual requiring a skin graft.

摘要翻译： 一种能够对人体皮肤进行治疗性治疗和/或体外测试的装置及其制造方法。 该装置可用于烫伤，受伤或患病皮肤的皮肤伤口，并提供如正常未受伤皮肤的结构和功能，例如屏障功能，这是正常皮肤的确定性质。 该装置在生物相容性可生物降解的网状基质上含有培养的皮肤和表皮细胞。 从培养的皮肤装置的受体中，全部或部分细胞可以是自体的，其有利地消除了组织相容性的问题。 细胞也可以在遗传上修饰以提供一个或多个因素以促进植入的皮肤置换物的愈合，例如用于刺激血管生长的血管生成因子。 本发明的装置易于处理和操作用于手术移植，可以制成大片以最小化覆盖待治疗的大表面积所需的移植物的数量，并且可以在时间框架内生产以治疗需要 皮肤移植。

公开(公告)号：US07741116B2

公开(公告)日：2010-06-22

申请号：US10092237

申请日：2002-03-06

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： C12N5/06 ,  C12N5/02 ,  C12N5/08

CPC分类号： A61F2/105 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323

摘要： A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels. The inventive device is easy to handle and manipulate for surgical transplant, can be made into large sheets to minimize the number of grafts required to cover a large surface area to be treated, and can be produced within the time frame to treat a burned individual requiring a skin graft.

摘要翻译： 一种能够对人体皮肤进行治疗性治疗和/或体外测试的装置及其制造方法。 该装置可用于烫伤，受伤或患病皮肤的皮肤伤口，并提供如正常未受伤皮肤的结构和功能，例如屏障功能，这是正常皮肤的确定性质。 该装置在生物相容性可生物降解的网状基质上含有培养的皮肤和表皮细胞。 从培养的皮肤装置的受体中，全部或部分细胞可以是自体的，其有利地消除了组织相容性的问题。 细胞也可以在遗传上修饰以提供一个或多个因素以促进植入的皮肤置换物的愈合，例如用于刺激血管生长的血管生成因子。 本发明的装置易于处理和操作用于手术移植，可以制成大片以最小化覆盖待治疗的大表面积所需的移植物的数量，并且可以在时间框架内生产以治疗需要 皮肤移植。

公开(公告)号：US20130259919A1

公开(公告)日：2013-10-03

申请号：US13901728

申请日：2013-05-24

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： A61F2/10 ,  A61L27/60

CPC分类号： A61F2/105 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323

摘要： A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels. The inventive device is easy to handle and manipulate for surgical transplant, can be made into large sheets to minimize the number of grafts required to cover a large surface area to be treated, and can be produced within the time frame to treat a burned individual requiring a skin graft.

摘要翻译： 一种能够对人体皮肤进行治疗性治疗和/或体外测试的装置及其制造方法。 该装置可用于烫伤，受伤或患病皮肤的皮肤伤口，并提供如正常未受伤皮肤的结构和功能，例如屏障功能，这是正常皮肤的确定性质。 该装置在生物相容性可生物降解的网状基质上含有培养的皮肤和表皮细胞。 从培养的皮肤装置的受体中，全部或部分细胞可以是自体的，其有利地消除了组织相容性的问题。 细胞也可以在遗传上修饰以提供一个或多个因素以促进植入的皮肤置换物的愈合，例如用于刺激血管生长的血管生成因子。 本发明的装置易于处理和操作用于手术移植，可以制成大片以最小化覆盖待治疗的大表面积所需的移植物的数量，并且可以在时间框架内生产以治疗需要 皮肤移植。

公开(公告)号：US20090111181A1

公开(公告)日：2009-04-30

申请号：US12257056

申请日：2008-10-23

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： C12N5/06

CPC分类号： A61L27/60 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3886

摘要： An apparatus and method of using the apparatus to prepare a biocompatible biodegradable matrix capable of supporting cells to form an implantable or engraftable surgical device. A matrix-forming fluid is contained within a chamber defined by top and bottom surfaces of a thermally conductive material and spacers defining the thickness of the matrix. The chamber is then cooled to freeze the solution at a controlled rate, resulting in a matrix with a desired and uniform thickness having symmetric and uniform reticulations. The apparatus and method reproducibly forms such a matrix, which may be populated with cells for transplantation and engraftment into a wound.

摘要翻译： 一种使用该装置制备能够支持细胞以形成可植入或可植入手术装置的生物相容性生物可降解基质的装置和方法。 基体形成流体包含在由导热材料的顶表面和底表面限定的室中，并且限定基体的厚度。 然后将腔室冷却以受控的速率冷冻溶液，得到具有所需和均匀厚度的基质，具有对称和均匀的网状结构。 该装置和方法可重复地形成这样的基质，其可以填充有用于移植和移入伤口的细胞。

公开(公告)号：US07452720B2

公开(公告)日：2008-11-18

申请号：US11142950

申请日：2005-06-02

申请人： Steven T. Boyce

发明人： Steven T. Boyce

IPC分类号： C12N5/00

CPC分类号： A61L27/60 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3886

摘要： An apparatus and method of using the apparatus to prepare a biocompatible biodegradable matrix capable of supporting cells to form an implantable or engraftable surgical device. A matrix-forming fluid is contained within a chamber defined by top and bottom surfaces of a thermally conductive material and spacers defining the thickness of the matrix. The chamber is then cooled to freeze the solution at a controlled rate, resulting in a matrix with a desired and uniform thickness having symmetric and uniform reticulations. The apparatus and method reproducibly forms such a matrix, which may be populated with cells for transplantation and engraftment into a wound.

摘要翻译： 一种使用该装置制备能够支持细胞以形成可植入或可植入手术装置的生物相容性生物可降解基质的装置和方法。 基体形成流体包含在由导热材料的顶表面和底表面限定的室中，并且限定基体的厚度。 然后将腔室冷却以受控的速率冷冻溶液，得到具有所需和均匀厚度的基质，具有对称和均匀的网状结构。 该装置和方法可重复地形成这样的基质，其可以填充有用于移植和移入伤口的细胞。