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    Selective isolation and concentration of nucleic acids from complex samples
    2.
    发明申请
    Selective isolation and concentration of nucleic acids from complex samples 审中-公开
    来自复杂样品的核酸的选择性分离和浓缩

    公开(公告)号：US20070154903A1

    公开(公告)日：2007-07-05

    申请号：US11473996

    申请日：2006-06-23

    申请人： Sudhakar Marla ,  Anna Prokhorova ,  Susan Hetzel ,  William Cork

    发明人： Sudhakar Marla ,  Anna Prokhorova ,  Susan Hetzel ,  William Cork

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： B82Y10/00 ,  B82Y5/00 ,  C12N1/06 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q2537/125 ,  C12Q2527/125

    摘要： The present invention provides methods for detecting a target nucleic acid molecule in a sample that comprises nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules in a complex environment containing numerous non-nucleic acid components. In particular, the present invention provides methods and probes for isolating DNA with detergents and detecting a single nucleotide polymorphism (SNP) in a complex sample that comprises numerous non-nucleic acid components and nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules.

    摘要翻译： 本发明提供了检测样品中靶核酸分子的方法，该方法包含比包含许多非核酸组分的复杂环境中的扩增核酸分子具有更高生物学复杂性的核酸分子。 特别地，本发明提供了用洗涤剂分离DNA并检测复合物样品中的单核苷酸多态性（SNP）的方法和探针，该复合物样品包含比扩增的核酸更高的生物学复杂性的许多非核酸组分和核酸分子 分子。

    Substrate functionalization method for high sensitivity applications
    3.
    发明申请
    Substrate functionalization method for high sensitivity applications 审中-公开
    用于高灵敏度应用的基板功能化方法

    公开(公告)号：US20060281076A1

    公开(公告)日：2006-12-14

    申请号：US11436395

    申请日：2006-05-18

    申请人： Sudhakar Marla ,  Yijia Bao ,  Martin Huber

    发明人： Sudhakar Marla ,  Yijia Bao ,  Martin Huber

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  G01N33/53 ,  C12M1/34 ,  B05D3/02

    CPC分类号： G01N33/54393 ,  G01N33/54353

    摘要： A method for functionalizing substrates such as magnetic beads and glass slides is provided. The method involves providing substrate surfaces with activated polyacrylic acid (PAA) and attaching one or more desired capture probes. Substrates prepared by the method are particularly useful in ultrasensitive detection assays as they exhibit very low specific binding and high binding efficiency relative to conventional substrates.

    摘要翻译： 提供了一种用于功能化基底例如磁珠和载玻片的方法。 该方法包括向基质表面提供活化的聚丙烯酸（PAA）并附着一种或多种所需的捕获探针。 通过该方法制备的底物在超灵敏检测测定中特别有用，因为它们相对于常规底物显示出非常低的特异性结合和高结合效率。