公开(公告)号：US20130196404A1

公开(公告)日：2013-08-01

申请号：US13359797

申请日：2012-01-27

申请人： Sumihiro Koyama ,  Tadashi Maruyama ,  Chiaki Kato ,  Yuichi Nogi ,  Yuji Hatada ,  Yukari Ohta ,  Masaaki Konishi ,  Taishi Tsubouchi

发明人： Sumihiro Koyama ,  Tadashi Maruyama ,  Chiaki Kato ,  Yuichi Nogi ,  Yuji Hatada ,  Yukari Ohta ,  Masaaki Konishi ,  Taishi Tsubouchi

IPC分类号： C12N13/00

CPC分类号： C12N13/00 ,  C12N11/14

摘要： A method for immobilizing living microorganisms includes a step (1) of disposing a solution containing microorganisms as an electrolyte on the surface of a substrate at least one portion of which is an electrode, and applying a constant potential to the electrode to cause at least a portion of the microorganisms to attach to the surface of the substrate. The constant potential in step (1) is greater than −0.5 V but not greater than −0.2 V (vs Ag/AgCl) or greater than +0.2 V but not greater than +0.4 V (vs Ag/AgCl). The electrolyte in step (1) does not contain a source of nutrition for the microorganisms.

摘要翻译： 固定活体微生物的方法包括将含有微生物作为电解质的溶液设置在基材的表面上的步骤（1），其中至少一部分是电极，并向电极施加恒定电位，使至少 部分微生物附着到基底表面。 步骤（1）中的恒定电位大于-0.5V但不大于-0.2V（相对于Ag / AgCl）或大于+ 0.2V但不大于+0.4V（相对于Ag / AgCl）。 步骤（1）中的电解质不含微生物的营养来源。

公开(公告)号：US08790907B2

公开(公告)日：2014-07-29

申请号：US13359797

申请日：2012-01-27

申请人： Sumihiro Koyama ,  Tadashi Maruyama ,  Chiaki Kato ,  Yuichi Nogi ,  Yuji Hatada ,  Yukari Ohta ,  Masaaki Konishi ,  Taishi Tsubouchi

发明人： Sumihiro Koyama ,  Tadashi Maruyama ,  Chiaki Kato ,  Yuichi Nogi ,  Yuji Hatada ,  Yukari Ohta ,  Masaaki Konishi ,  Taishi Tsubouchi

IPC分类号： C12N1/00 ,  C12N1/04 ,  C12N11/14

CPC分类号： C12N13/00 ,  C12N11/14

摘要： A method for immobilizing living microorganisms includes a step (1) of disposing a solution containing microorganisms as an electrolyte on the surface of a substrate at least one portion of which is an electrode, and applying a constant potential to the electrode to cause at least a portion of the microorganisms to attach to the surface of the substrate. The constant potential in step (1) is greater than −0.5 V but not greater than −0.2 V (vs Ag/AgCl) or greater than +0.2 V but not greater than +0.4 V (vs Ag/AgCl). The electrolyte in step (1) does not contain a source of nutrition for the microorganisms.

摘要翻译： 固定活体微生物的方法包括将含有微生物作为电解质的溶液设置在基材的表面上的步骤（1），其中至少一部分是电极，并向电极施加恒定电位，使至少 部分微生物附着到基底表面。 步骤（1）中的恒定电位大于-0.5V但不大于-0.2V（相对于Ag / AgCl）或大于+ 0.2V但不大于+0.4V（相对于Ag / AgCl）。 步骤（1）中的电解质不含微生物的营养来源。

公开(公告)号：US20120264186A1

公开(公告)日：2012-10-18

申请号：US13504513

申请日：2010-10-29

申请人： Sumihiro Koyama

发明人： Sumihiro Koyama

IPC分类号： C12N13/00

CPC分类号： C12N5/00 ,  C12M25/08 ,  C12M33/00 ,  C12M35/02 ,  C12N2529/00 ,  C12N2539/10

摘要： A method for preparing animal cells with good adhesion and proliferation properties which are capable of proliferation is provided. the method permits the detachment of cultured cells without damaging the cells. A method for preparing a sheet of animal cells such as skin cells which are capable of proliferation is also provided. A method for preparing animal cells capable of proliferation, comprising the steps of (1) culturing animal cells on a substrate at least one portion of which is an electrode, and (2) applying a high-frequency wave potential to the electrode to detach the cells that have adhered to the substrate surface through culturing. The high-frequency wave potential is of a frequency falling within a range of 1 KHz to 10 MHz with a potential falling within a range of ±1.0 V (vs. Ag/AgCl) or less. The culture medium during separation does not contain calcium or magnesium.

摘要翻译： 提供了一种制备能够增殖的具有良好粘附性和增殖性能的动物细胞的方法。 该方法允许培养细胞的分离而不破坏细胞。 还提供了一种制备能够增殖的皮肤细胞片等动物细胞的方法。 一种制备能够增殖的动物细胞的方法，包括以下步骤：（1）在至少一部分电极上的基底上培养动物细胞，（2）向所述电极施加高频波电位， 通过培养粘附于基材表面的细胞。 高频波电位为1KHz〜10MHz的范围，电位落在±1.0V（vs.Ag / AgCl）以下的范围内。 分离过程中的培养基不含钙或镁。

公开(公告)号：US20120270755A1

公开(公告)日：2012-10-25

申请号：US13504509

申请日：2010-10-29

申请人： Sumihiro Koyama

发明人： Sumihiro Koyama

IPC分类号： C40B50/06

CPC分类号： C12N5/0068 ,  C12N2529/00 ,  C12N2533/90

摘要： A new means of separately arranging individual animal cells on a substrate surface is provided. A method for preparing a substrate for arranging animal cells in an array, comprising steps (1) to (3): (1) preparing a substrate having adsorption surfaces in an array on an electrode substrate surface; (2) causing an extracellular matrix to adsorb to the electrode surface and the adsorption surfaces of the substrate; and (3) applying a weak potential to the electrode to cause the extracellular matrix on the electrode surface to separate to obtain a substrate with the extracellular matrix adhered to the adsorption surfaces thereof. A method for preparing a substrate on which animal cells have been arranged in an array, comprising steps (1) to (4): (1) preparing a substrate having adsorption surfaces in an array on an electrode substrate surface; (2) causing an extracellular matrix to adsorb to the electrode surface and the adsorption surfaces of the substrate; (3) applying a weak potential to the electrode to cause the extracellular matrix on the electrode surface to separate to obtain a substrate with extracellular matrix adhered to the adsorption surfaces thereof; and (4) culturing the animal cells on the surface of the substrate obtained in (3) to obtain a substrate on which the animal cells have adhered to the adsorption surface.

摘要翻译： 提供了一种在基片表面上分别布置单个动物细胞的新方法。 一种制备用于将阵列中的动物细胞排列的衬底的方法，包括步骤（1）至（3）：（1）在电极衬底表面上制备阵列中具有吸附表面的衬底; （2）使细胞外基质吸附到基板的电极表面和吸附面上; （3）向电极施加弱电位使电极表面上的细胞外基质分离，得到附着于其吸附表面的细胞外基质的底物。 一种制备其上已经排列有动物细胞的基板的方法，包括步骤（1）至（4）：（1）在电极基板表面上制备阵列中具有吸附表面的基板; （2）使细胞外基质吸附到基板的电极表面和吸附面上; （3）向电极施加弱电位使电极表面上的细胞外基质分离，得到附着于其吸附表面的细胞外基质的底物; （4）在（3）得到的基材的表面上培养动物细胞，得到动物细胞附着于吸附面的基材。