Method and apparatus for sample preparation
    1.
    发明申请
    Method and apparatus for sample preparation 审中-公开
    样品制备方法和装置

    公开(公告)号:US20080241841A1

    公开(公告)日:2008-10-02

    申请号:US11984065

    申请日:2007-11-13

    IPC分类号: C12Q1/68 C12P21/04 C12M1/34

    摘要: A method of the present invention comprises fractionating a sample solution containing analyte DNA molecules into small droplets, wherein the number M of the droplets is greater than the total number N of the DNA molecules, subjecting an emulsion containing the droplets to, for example, PCR amplification, and detecting the presence or absence (amount) of an amplicon obtained in each droplet by fluorescent detection using an intercalator or the like.

    摘要翻译: 本发明的方法包括将含有分析物DNA分子的样品溶液分级成小液滴,其中液滴的数量M大于DNA分子的总数N,使含有液滴的乳液经受例如PCR 通过使用嵌入剂等进行荧光检测,检测各液滴中获得的扩增子的存在或不存在(量)。