公开(公告)号：US07198936B2

公开(公告)日：2007-04-03

申请号：US10415332

申请日：2001-10-30

申请人： Gustav Silfversparre ,  Sven-Olof Enfors ,  Ling Han ,  Lena Häggström ,  Harald Skogman

发明人： Gustav Silfversparre ,  Sven-Olof Enfors ,  Ling Han ,  Lena Häggström ,  Harald Skogman

IPC分类号： C12N1/12 ,  C12N1/20

CPC分类号： C12N1/20

摘要： The invention relates to a method for growth of bacteria, such as Gram negative bacteria and its corresponding supernatant. The bacteria are cultivated without substantial release of endotoxins from the bacteria into the surrounding medium. Thereby the need of purification steps necessary to remove endotoxins are reduced or eliminated such as when the bacteria are producing a product which later on should be administered to a mammal, such as a human. The bacteria are also cultivated without substantial formation of acetic acid. The level of the carbon/energy source is controled during the cultivation and oxygen limitation is avoided through regulation of the temperature. The method may be used for cultivation of bacterial in fermenters.

摘要翻译： 本发明涉及细菌生长方法，如革兰氏阴性菌及其相应的上清液。 培养细菌而没有从细菌中释放内毒素到周围培养基中。 因此，减少或消除了除去内毒素所需的纯化步骤的需要，例如当细菌生产稍后应该给予哺乳动物（例如人）的产品时。 细菌也培养而没有实质形成乙酸。 在培养期间控制碳/能源的水平，通过调节温度来避免氧气限制。 该方法可用于在发酵罐中培养细菌。

公开(公告)号：US4374013A

公开(公告)日：1983-02-15

申请号：US239979

申请日：1981-03-03

申请人： Sven-Olof Enfors

发明人： Sven-Olof Enfors

IPC分类号： G01N27/416 ,  C12Q1/00 ,  C12Q1/26

CPC分类号： C12Q1/005 ,  Y10S435/817

摘要： An enzyme electrode based on an oxygen electrode and an oxygen consuming enzyme is described. The oxygen demand of the electrode is satisfied through an electrolytical oxygen production by means of an anode located close to the enzyme and the sensitive surface of the oxygen electrode. The deviation from a reference value, which could be constant or be formed by the signal from an enzymatically passive oxygen electrode, of the oxygen electrode signal, is used to control the electrolysis current in order to keep the oxygen activity in the enzyme electrode constant. The electrolysis current is used as a measure of the concentration of the substrate of the enzyme.

摘要翻译： 描述了基于氧电极和耗氧酶的酶电极。 通过利用位于酶附近的阳极和氧电极的敏感表面进行电解氧生成来满足电极的氧需求。 使用与氧电极信号的来自酶惰性氧电极的信号恒定或形成的参考值的偏差来控制电解电流，以保持酶电极中的氧活性恒定。 电解电流用作酶底物浓度的量度。

公开(公告)号：US4346175A

公开(公告)日：1982-08-24

申请号：US948275

申请日：1978-10-03

申请人： Sven-Olof Enfors ,  Nils Molin ,  Eugen Montelin

发明人： Sven-Olof Enfors ,  Nils Molin ,  Eugen Montelin

IPC分类号： C12N1/00 ,  B27K5/00 ,  B27K5/02 ,  C12P1/00 ,  C12P1/02

CPC分类号： B27K3/002 ,  B27K5/02 ,  Y10S435/838 ,  Y10S435/911

摘要： The disclosure relates to a process for the microbiological modification of hardwood in that a microorganism, or an enzymatically active preparation thereof, specially selected for the hardwood in question, is supplied to the surface of the wood for attack thereof under carefully regulated conditions as regards temperature, relative moisture content, O.sub.2 content and CO.sub.2 content. Thereafter, the process is discontinued in a suitable manner, for example by treatment with steam or hot water, followed by drying; or by drying only.

摘要翻译： 本公开涉及一种用于对硬木进行微生物修饰的方法，其中特别选择用于所述硬木的微生物或其酶促活性制剂在严格限制的温度条件下供应到木材表面以进行攻击 ，相对含水量，O2含量和CO2含量。 此后，以适当的方式停止该方法，例如通过用蒸汽或热水处理，然后干燥; 或仅通过干燥。

公开(公告)号：US4024042A

公开(公告)日：1977-05-17

申请号：US667864

申请日：1976-03-17

申请人： Sven Olof Enfors ,  Nils Ludvig Molin ,  Klaus Hermann Mosbach ,  Hans Jorgen Nilsson

发明人： Sven Olof Enfors ,  Nils Ludvig Molin ,  Klaus Hermann Mosbach ,  Hans Jorgen Nilsson

IPC分类号： C12Q1/00 ,  G01N27/46 ,  C12K1/04

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： The invention relates to an enzyme electrode which can be sterilized and regenerated and which has a sensitive surface and an enzyme in contact therewith. A semipermeable membrane is arranged at a distance from the sensitive surface of the detecting electrode and defines a chamber containing the enzyme which is supplied to and discharged from the chamber through a supply conduit and a discharge conduit, respectively. A sterilization device for the enzyme is arranged in the supply conduit for the enzyme. The enzyme electrode is produced by means of the detecting electrode being sterilized in a manner known per se and by means of the enzyme being led into a chamber via a sterilization device through a supply conduit for contact with the sensitive surface of the detecting electrode and for contact with the semipermeable membrane arranged at a distance from said sensitive surface. The enzyme is continuously or intermittently supplied through the supply conduit simultaneously as previously supplied enzyme is discharged from the chamber through the discharge conduit.

摘要翻译： 本发明涉及可以灭菌和再生并且具有敏感表面和与之接触的酶的酶电极。 半透膜设置在与检测电极的敏感表面相距一定距离处，并且限定了含有酶的室，其分别通过供应管道和排出管道供应到室并从室排出。 用于酶的消毒装置设置在用于酶的供应管道中。 酶电极通过以本身已知的方式进行灭菌的检测电极产生，并且通过经由灭菌装置的酶被引导到室中，通过供应导管与检测电极的敏感表面接触，并且 与设置在距离所述敏感表面一定距离处的半透膜接触。 酶通过供应管道同时连续或间歇地供应，因为先前供应的酶通过排出导管从室排出。