Method for production of a vegetable protein hydrolyzate with proteases
    1.
    发明授权
    Method for production of a vegetable protein hydrolyzate with proteases 失效
    用蛋白酶生产植物蛋白水解产物的方法

    公开(公告)号:US5716801A

    公开(公告)日:1998-02-10

    申请号:US417715

    申请日:1995-04-06

    CPC分类号: A23J3/346

    摘要: A well tasting and organoleptically acceptable vegetable protein hydrolyzate such as soy, pea or rice protein hydrolyzate is produced in high yield by a method using a combination of non-pH-stat hydrolysis and ultrafiltration. Preferably, the method is carried out by mixing a material containing at least 65% vegetable protein as dry matter and water to form a slurry containing a vegetable protein content of about 7-20%, heating the slurry to above 60.degree. C., adjusting the pH of the slurry to about 8.5, hydrolyzing the slurry with at least two different proteases to a degree of hydrolysis of between 15 and 35% without adjusting the pH during hydrolysis to produce a hydrolyzed slurry, inactivating the proteases and separating the hydrolyzed slurry with an ultrafiltration unit having a cut-off value above 5,000 to form a permeate containing the vegetable protein hydrolyzate. One protease may be obtained from B. Licheniformis and the other from B. Subtilis. The hydrolyzed slurry or the permeate may be treated with activated carbon, and the permeate may be concentrated by nanofiltration and/or by evaporation.

    摘要翻译: 通过使用非pH值水解和超滤的组合的方法,以高产率生产出品尝和感官可接受的植物蛋白水解产物如大豆,豌豆或米蛋白水解产物。 优选地,通过将​​含有至少65%植物蛋白作为干物质的材料和水混合以形成含有约7-20%的植物蛋白质含量的浆料,将浆料加热至60℃以上,调节 将浆料的pH调节至约8.5,用至少两种不同蛋白酶将浆料水解至15至35%之间的水解度,而不调节水解期间的pH以产生水解浆液,使蛋白酶失活并将水解的浆液与 具有超过5000的截止值的超滤单元以形成含有植物蛋白水解产物的渗透物。 一种蛋白酶可以从地衣芽孢杆菌获得,另一种来自枯草芽孢杆菌。 可以用活性炭处理水解的浆液或渗透物,并且可以通过纳滤和/或通过蒸发浓缩渗透物。

    Method for hydrolyzing proteins with gluyasp specific protease
    2.
    发明授权
    Method for hydrolyzing proteins with gluyasp specific protease 失效
    用gluyasp特异性蛋白酶水解蛋白质的方法

    公开(公告)号:US5866357A

    公开(公告)日:1999-02-02

    申请号:US927661

    申请日:1992-08-31

    CPC分类号: A23J3/34

    摘要: A method is disclosed for obtaining limited specific hydrolysis of proteins using a Glu/Asp specific protease to produce peptides with C-terminal Glu or Asp. The protease is obtained from Bacillus licheniformis, has a mass of about 23,600, is inhibited by diisopropyl fluoride but not by phenylmethane sulfonylfluoride, and has a pH of maximal activity between 6.5 and 10.0. The proteins are treated with the protease at neutral pH and the reaction terminated by raising the temperature above 70.degree. C. or lowering the pH below 5.0 to obtain the hydrolysate. The process may include a second protease which is specific for Arg/Lys, and produces peptides with C-terminal Arg or Lys.

    摘要翻译: PCT No.PCT / DK91 / 00069 Sec。 371日期:1992年8月31日 102(e)日期1992年8月31日PCT 1991年3月8日PCT公布。 公开号WO91 / 13554 1991年9月19日公开了一种用于使用Glu / Asp特异性蛋白酶获得有限的蛋白质特异性水解以产生具有C端Glu或Asp的肽的方法。 蛋白酶从地衣芽孢杆菌获得,质量约为23,600,被二异丙基氟化物抑制,但不被苯基甲烷磺酰氟抑制,并且具有6.5至10.0之间最大活性的pH。 蛋白质在中性pH下用蛋白酶处理,并且通过将温度升高至70℃以上或将pH降低至5.0以终止反应,得到水解产物。 该方法可以包括对Arg / Lys特异的第二种蛋白酶,并产生具有C末端Arg或Lys的肽。

    Method for production of a whey protein hydrolyzate
    3.
    发明授权
    Method for production of a whey protein hydrolyzate 失效
    生产乳清蛋白水解物的方法

    公开(公告)号:US5691165A

    公开(公告)日:1997-11-25

    申请号:US157074

    申请日:1993-11-30

    CPC分类号: A23J3/343

    摘要: A well tasting and organoleptically acceptable whey protein hydrolyzate is produced in high yield by a method using a combination of non-pH-stat hydrolysis and ultrafiltration. Preferably, the method is carried out by mixing a material containing at least 65% whey protein as dry matter and water to form a slurry containing a whey protein content of about 7-20%, heating the slurry to above 600.degree. C., adjusting the pH of the slurry to about 8, hydrolyzing the slurry with at least two different proteases to a degree of hydrolysis of between 17 and 35% without adjusting the pH during hydrolysis to produce a hydrolyzed slurry, inactivating the proteases and separating the hydrolyzed slurry with an ultrafiltration unit having a cut-off value above 10,000 to form a permeate containing the whey protein hydrolyzate. One protease may be obtained from B. Licheniformis and the other from B. Subtilis. The hydrolyzed slurry or the permeate may be treated with activated carbon, and the permeate may be concentrated by nanofiltration and/or by evaporation.

    摘要翻译: PCT No.PCT / DK92 / 00170 Sec。 371日期:1993年11月30日 102(e)1993年11月30日日期PCT提交1992年5月27日PCT公布。 出版物WO92 / 21248 日期1992年12月10日通过使用非pH值水解和超滤的组合的方法,以高产率生产品鉴和感官可接受的乳清蛋白水解产物。 优选地,通过将​​含有至少65%的乳清蛋白作为干物质的材料和水混合以形成含有约7-20%的乳清蛋白质含量的浆料,将浆料加热至600℃以上,调节 将浆料的pH调节至约8,用至少两种不同蛋白酶将浆料水解至17-35%之间的水解度,而不调节水解期间的pH,从而产生水解浆液,使蛋白酶失活并将水解的浆液与 具有高于10,000的临界值的超滤单元以形成含有乳清蛋白水解产物的渗透物。 一种蛋白酶可以从地衣芽孢杆菌获得,另一种来自枯草芽孢杆菌。 可以用活性炭处理水解的浆液或渗透物,并且可以通过纳滤和/或通过蒸发浓缩渗透物。

    Method for production of pea protein hydrolyzate
    5.
    发明授权
    Method for production of pea protein hydrolyzate 失效
    豌豆蛋白水解产物的生产方法

    公开(公告)号:US5520935A

    公开(公告)日:1996-05-28

    申请号:US107690

    申请日:1993-08-18

    CPC分类号: C12P21/06 A23J3/346 A23L33/18

    摘要: The present invention relates to a pea protein hydrolyzate with very high purity and with organoleptic properties. The present invention also relates to a method for producing said pea protein hydrolyzate.

    摘要翻译: PCT No.PCT / DK92 / 00069 Sec。 371日期:1992年8月18日 102(e)日期1992年8月18日PCT提交1992年3月6日PCT公布。 公开号WO92 / 15697 本发明涉及具有非常高纯度和感官特性的豌豆蛋白水解产物。 本发明还涉及一种生产所述豌豆蛋白水解产物的方法。