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    LIQUID CHROMATOGRAPHY SYSTEM AND METHOD FOR PROTEIN SEPARATION AND PURIFICATION
    1.
    发明申请
    LIQUID CHROMATOGRAPHY SYSTEM AND METHOD FOR PROTEIN SEPARATION AND PURIFICATION 审中-公开
    液相色谱系统和蛋白质分离和纯化方法

    公开(公告)号：US20130109102A1

    公开(公告)日：2013-05-02

    申请号：US13237326

    申请日：2011-09-20

    申请人： HIROYUKI YABE ,  TOMOYUKI MIYABAYASHI

    发明人： MICHAEL LI ,  HIROYUKI YABE ,  TOMOYUKI MIYABAYASHI

    IPC分类号： G01N31/16

    CPC分类号： G01N31/16 ,  B01D15/166 ,  B01D15/361 ,  C07K1/18 ,  G01N30/34 ,  Y10T436/116664

    摘要： A system and method for protein separation and purification includes a mixing loop, a primary pump having an inlet in communication with a supply of an acid or an alkali and an outlet in communication with the mixing loop. A concentrate pump has an inlet in communication with a supply of an acid or an alkali and an outlet in communication with the mixing loop. A liquid chromatography column is in communication with the mixing loop and is packed with an ion exchange resin and loaded with a protein solution containing a first protein having a first isoelectric point and a second protein having a second isoelectric point. A process control feedback loop in communication with the mixing loop includes an inline process pH analyzer so that a pH of a titration solution in the mixing loop may be detected and adjusted based on the detected pH. The titration solution is provided in the mixing loop by the primary and concentrate pumps and the pH of the titration solution in the mixing loop is adjusted by the feedback loop based on the pH detected by the inline process pH analyzer to provide a generally linear pH gradient to the chromatography column so that the first and second proteins are separately eluded from the liquid chromatography column.

    摘要翻译： 用于蛋白质分离和纯化的系统和方法包括混合回路，具有与酸或碱供应源连通的入口和与混合回路连通的出口的初级泵。 浓缩泵具有与酸或碱供应源连接的入口和与混合回路连通的出口。 液相色谱柱与混合回路连通，并填充有离子交换树脂，并装载含有具有第一等电点的第一蛋白质和具有第二等电点的第二蛋白质的蛋白质溶液。 与混合回路连通的过程控制反馈回路包括在线过程pH分析仪，使得可以基于检测到的pH来检测和调整混合回路中的滴定溶液的pH。 滴定溶液通过初级浓缩泵和浓缩泵在混合回路中提供，并且基于通过在线工艺pH分析仪检测的pH通过反馈回路调节混合回路中的滴定溶液的pH，以提供通常的线性pH梯度 到色谱柱，使得第一和第二种蛋白质分离地从液相色谱柱中除去。

    Cell differentiation suppressing agent, method of culturing cells using the same, culture solution, and cultured cell line
    2.
    发明授权
    Cell differentiation suppressing agent, method of culturing cells using the same, culture solution, and cultured cell line 有权
    细胞分化抑制剂，使用其的培养细胞的培养方法，培养液和培养细胞系

    公开(公告)号：US08883504B2

    公开(公告)日：2014-11-11

    申请号：US12233252

    申请日：2008-09-18

    申请人： Tomoyuki Miyabayashi ,  Masashi Yamamoto

    发明人： Tomoyuki Miyabayashi ,  Masashi Yamamoto

    IPC分类号： C12N5/00 ,  C07D217/16 ,  A61K31/47 ,  A61K31/404 ,  A61K31/655 ,  A61K31/472

    CPC分类号： C07D217/16 ,  A61K31/404 ,  A61K31/47 ,  A61K31/472 ,  A61K31/655 ,  C12N5/0018 ,  C12N2501/999

    摘要： The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.

    摘要翻译： 本发明的目的在于提供一种分化抑制剂，其能够不使用任何饲养细胞的方式培养未分化状态的干细胞或胚胎干细胞，使用其的培养方法，使用 和通过使用这种分化抑制剂进行培养而制备的细胞。 本发明提供一种分解抑制剂，其包含低分子量化合物，特别是四氢异喹啉衍生物作为活性成分; 在不存在饲养细胞的情况下，以未分化状态大规模安装培养干细胞的方法，包括使用四氢异喹啉衍生物培养干细胞; 包含四氢异喹啉衍生物的干细胞培养液; 以及通过使用四氢异喹啉衍生物作为分化抑制剂的培养获得的细胞。

    CELL DIFFERENTIATION SUPPRESSING AGENT, METHOD OF CULTURING CELLS USING THE SAME, CULTURE SOLUTION, AND CULTURED CELL LINE
    4.
    发明申请
    CELL DIFFERENTIATION SUPPRESSING AGENT, METHOD OF CULTURING CELLS USING THE SAME, CULTURE SOLUTION, AND CULTURED CELL LINE 有权
    细胞分化抑制剂，使用该细胞培养细胞的方法，文化解决方案和培养的细胞系

    公开(公告)号：US20090155906A1

    公开(公告)日：2009-06-18

    申请号：US12233252

    申请日：2008-09-18

    申请人： Tomoyuki Miyabayashi ,  Masashi Yamamoto

    发明人： Tomoyuki Miyabayashi ,  Masashi Yamamoto

    IPC分类号： C12N5/06

    CPC分类号： C07D217/16 ,  A61K31/404 ,  A61K31/47 ,  A61K31/472 ,  A61K31/655 ,  C12N5/0018 ,  C12N2501/999

    摘要： The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.

    摘要翻译： 本发明的目的在于提供一种分化抑制剂，其能够不使用任何饲养细胞的方式培养未分化状态的干细胞或胚胎干细胞，使用其的培养方法，使用 和通过使用这种分化抑制剂进行培养而制备的细胞。 本发明提供一种分解抑制剂，其包含低分子量化合物，特别是四氢异喹啉衍生物作为活性成分; 在不存在饲养细胞的情况下，以未分化状态大规模安装培养干细胞的方法，包括使用四氢异喹啉衍生物培养干细胞; 包含四氢异喹啉衍生物的干细胞培养液; 以及通过使用四氢异喹啉衍生物作为分化抑制剂的培养获得的细胞。