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公开(公告)号:US07947813B2
公开(公告)日:2011-05-24
申请号:US11972010
申请日:2008-01-10
申请人: Robert L. Fahrner , Jayme Franklin , Paul McDonald , Thanmaya Peram , Vikram Sisodiya , Corazon Victa
发明人: Robert L. Fahrner , Jayme Franklin , Paul McDonald , Thanmaya Peram , Vikram Sisodiya , Corazon Victa
CPC分类号: C07K16/00 , C07K1/32 , C07K1/36 , C07K16/2803 , C07K16/2863 , C07K16/2878 , C07K16/2887 , C07K2317/24 , C07K2317/734
摘要: Methods are presented for isolating and purifying proteins by adding a polyelectrolyte to a cell culture fluid, such as a harvested cell culture fluid, and precipitating a protein-polyelectrolyte complex or a complex of impurities and the polyelectrolyte.
摘要翻译: 提出了通过向细胞培养液(例如收获的细胞培养液)中加入聚电解质和沉淀蛋白质 - 聚电解质络合物或杂质与聚电解质络合物来分离和纯化蛋白质的方法。
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公开(公告)号:US20080193981A1
公开(公告)日:2008-08-14
申请号:US11972010
申请日:2008-01-10
申请人: Robert L. Fahrner , Jayme Franklin , Paul McDonald , Thanmaya Peram , Vikram Sisodiya , Corazon Victa
发明人: Robert L. Fahrner , Jayme Franklin , Paul McDonald , Thanmaya Peram , Vikram Sisodiya , Corazon Victa
IPC分类号: C12P21/04
CPC分类号: C07K16/00 , C07K1/32 , C07K1/36 , C07K16/2803 , C07K16/2863 , C07K16/2878 , C07K16/2887 , C07K2317/24 , C07K2317/734
摘要: Methods are presented for isolating and purifying proteins by adding a polyelectrolyte to a cell culture fluid, such as a harvested cell culture fluid, and precipitating a protein-polyelectrolyte complex or a complex of impurities and the polyelectrolyte.
摘要翻译: 提出了通过向细胞培养液(例如收获的细胞培养液)中加入聚电解质和沉淀蛋白质 - 聚电解质络合物或杂质与聚电解质络合物来分离和纯化蛋白质的方法。
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