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公开(公告)号:US07947813B2
公开(公告)日:2011-05-24
申请号:US11972010
申请日:2008-01-10
申请人: Robert L. Fahrner , Jayme Franklin , Paul McDonald , Thanmaya Peram , Vikram Sisodiya , Corazon Victa
发明人: Robert L. Fahrner , Jayme Franklin , Paul McDonald , Thanmaya Peram , Vikram Sisodiya , Corazon Victa
CPC分类号: C07K16/00 , C07K1/32 , C07K1/36 , C07K16/2803 , C07K16/2863 , C07K16/2878 , C07K16/2887 , C07K2317/24 , C07K2317/734
摘要: Methods are presented for isolating and purifying proteins by adding a polyelectrolyte to a cell culture fluid, such as a harvested cell culture fluid, and precipitating a protein-polyelectrolyte complex or a complex of impurities and the polyelectrolyte.
摘要翻译: 提出了通过向细胞培养液(例如收获的细胞培养液)中加入聚电解质和沉淀蛋白质 - 聚电解质络合物或杂质与聚电解质络合物来分离和纯化蛋白质的方法。
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公开(公告)号:US20080193981A1
公开(公告)日:2008-08-14
申请号:US11972010
申请日:2008-01-10
申请人: Robert L. Fahrner , Jayme Franklin , Paul McDonald , Thanmaya Peram , Vikram Sisodiya , Corazon Victa
发明人: Robert L. Fahrner , Jayme Franklin , Paul McDonald , Thanmaya Peram , Vikram Sisodiya , Corazon Victa
IPC分类号: C12P21/04
CPC分类号: C07K16/00 , C07K1/32 , C07K1/36 , C07K16/2803 , C07K16/2863 , C07K16/2878 , C07K16/2887 , C07K2317/24 , C07K2317/734
摘要: Methods are presented for isolating and purifying proteins by adding a polyelectrolyte to a cell culture fluid, such as a harvested cell culture fluid, and precipitating a protein-polyelectrolyte complex or a complex of impurities and the polyelectrolyte.
摘要翻译: 提出了通过向细胞培养液(例如收获的细胞培养液)中加入聚电解质和沉淀蛋白质 - 聚电解质络合物或杂质与聚电解质络合物来分离和纯化蛋白质的方法。
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3.
公开(公告)号:US07807799B2
公开(公告)日:2010-10-05
申请号:US12269752
申请日:2008-11-12
IPC分类号: C07K16/00
CPC分类号: C07K16/065
摘要: A method for reducing leaching of protein A during protein A affinity chromatography is described which involves reducing temperature or pH of, or by adding one or more protease inhibitors to, a composition that is subjected to protein A affinity chromatography.
摘要翻译: 描述了在蛋白A亲和层析期间减少蛋白质A浸出的方法,其涉及将经过蛋白A亲和层析的组合物的温度或pH值降低或加入一种或多种蛋白酶抑制剂。
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4.
公开(公告)号:US20090099344A1
公开(公告)日:2009-04-16
申请号:US12269752
申请日:2008-11-12
IPC分类号: C07K1/00
CPC分类号: C07K16/065
摘要: A method for reducing leaching of protein A during protein A affinity chromatography is described which involves reducing temperature or pH of, or by adding one or more protease inhibitors to, a composition that is subjected to protein A affinity chromatography.
摘要翻译: 描述了在蛋白A亲和层析期间减少蛋白质A浸出的方法,其涉及将经过蛋白A亲和层析的组合物的温度或pH值降低或加入一种或多种蛋白酶抑制剂。
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5.
公开(公告)号:US07485704B2
公开(公告)日:2009-02-03
申请号:US10877532
申请日:2004-06-24
IPC分类号: C07K16/00
CPC分类号: C07K16/065
摘要: A method for reducing leaching of protein A during protein A affinity chromatography is described which involves reducing temperature or pH of, or by adding one or more protease inhibitors to, a composition that is subjected to protein A affinity chromatography.
摘要翻译: 描述了在蛋白A亲和层析期间减少蛋白质A浸出的方法,其涉及将经过蛋白A亲和层析的组合物的温度或pH值降低或加入一种或多种蛋白酶抑制剂。
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公开(公告)号:US06870034B2
公开(公告)日:2005-03-22
申请号:US10356974
申请日:2003-02-03
申请人: Timothy N. Breece , Robert L. Fahrner , Jeffrey R. Gorrell , Kathlyn Pham Lazzareschi , Philip M. Lester , David Peng
发明人: Timothy N. Breece , Robert L. Fahrner , Jeffrey R. Gorrell , Kathlyn Pham Lazzareschi , Philip M. Lester , David Peng
CPC分类号: C07K1/22 , C07K16/065 , Y10S436/824 , Y10S436/828
摘要: A method for purifying proteins by Protein A chromatography is described which comprises removing contaminants by washing the solid phase with various intermediate wash buffers.
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