Kinase and phosphatase assays
    5.
    发明授权
    Kinase and phosphatase assays 有权
    激酶和磷酸酶测定

    公开(公告)号:US08487078B2

    公开(公告)日:2013-07-16

    申请号:US13252679

    申请日:2011-10-04

    IPC分类号: C07K7/00 C12N9/12

    摘要: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.

    摘要翻译: 描述了用于检测和监测激酶,磷酸酶和蛋白质翻译后修饰活性的组合物,方法和试剂盒。 组合物通常包括肽,可检测部分和蛋白酶切割位点。 通过激酶,磷酸酶或其他蛋白质翻译后修饰来修饰肽改变了肽的蛋白水解敏感性,导致组合物的可检测性质的变化。 还描述了用于确定激酶,磷酸酶或其他蛋白质翻译后修饰活性的底物或调节剂的面板测定法。

    KINASE AND PHOSPHATASE ASSAYS
    6.
    发明申请
    KINASE AND PHOSPHATASE ASSAYS 有权
    激酶和磷酸酶测定

    公开(公告)号:US20120083427A1

    公开(公告)日:2012-04-05

    申请号:US13252679

    申请日:2011-10-04

    摘要: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.

    摘要翻译: 描述了用于检测和监测激酶,磷酸酶和蛋白质翻译后修饰活性的组合物,方法和试剂盒。 组合物通常包括肽,可检测部分和蛋白酶切割位点。 通过激酶,磷酸酶或其他蛋白质翻译后修饰来修饰肽改变了肽的蛋白水解敏感性,导致组合物的可检测性质的改变。 还描述了用于确定激酶,磷酸酶或其他蛋白质翻译后修饰活性的底物或调节剂的面板测定法。

    Kinase and phosphatase assays
    8.
    发明授权
    Kinase and phosphatase assays 有权
    激酶和磷酸酶测定

    公开(公告)号:US08067536B2

    公开(公告)日:2011-11-29

    申请号:US12728926

    申请日:2010-03-22

    IPC分类号: C07K4/00 C12Q1/42

    摘要: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.

    摘要翻译: 描述了用于检测和监测激酶,磷酸酶和蛋白质翻译后修饰活性的组合物,方法和试剂盒。 组合物通常包括肽,可检测部分和蛋白酶切割位点。 通过激酶,磷酸酶或其他蛋白质翻译后修饰来修饰肽改变了肽的蛋白水解敏感性,导致组合物的可检测性质的变化。 还描述了用于确定激酶,磷酸酶或其他蛋白质翻译后修饰活性的底物或调节剂的面板测定法。

    Kinase and phosphatase assays
    10.
    发明申请
    Kinase and phosphatase assays 有权
    激酶和磷酸酶测定

    公开(公告)号:US20070059787A1

    公开(公告)日:2007-03-15

    申请号:US11485957

    申请日:2006-07-14

    IPC分类号: G01N33/53 C12Q1/48

    摘要: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.

    摘要翻译: 描述了用于检测和监测激酶,磷酸酶和蛋白质翻译后修饰活性的组合物,方法和试剂盒。 组合物通常包括肽,可检测部分和蛋白酶切割位点。 通过激酶,磷酸酶或其他蛋白质翻译后修饰来修饰肽改变了肽的蛋白水解敏感性,导致组合物的可检测性质的变化。 还描述了用于确定激酶,磷酸酶或其他蛋白质翻译后修饰活性的底物或调节剂的面板测定法。