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    Method for producing RNA-containing probe for detecting a target nucleotide
    1.
    发明授权
    Method for producing RNA-containing probe for detecting a target nucleotide 有权
    用于产生用于检测靶核苷酸的含RNA探针的方法

    公开(公告)号：US09336349B2

    公开(公告)日：2016-05-10

    申请号：US13812242

    申请日：2011-07-28

    申请人： Junko Yamamoto ,  Tomoo Inden ,  Toshiharu Ohba ,  Tooru Suzuki ,  Hiroyuki Mukai ,  Kiyozo Asada

    发明人： Junko Yamamoto ,  Tomoo Inden ,  Toshiharu Ohba ,  Tooru Suzuki ,  Hiroyuki Mukai ,  Kiyozo Asada

    IPC分类号： C12Q1/68 ,  G06F19/12 ,  G06F19/20

    CPC分类号： G06F19/12 ,  C12Q1/6816 ,  C12Q1/6876 ,  C12Q2527/107 ,  G06F19/20

    摘要： An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40° C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3′ or 5′ end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.

    摘要翻译： 本发明的目的在于提供一种用于检测靶核苷酸的含RNA探针的简单且有用的方法，用于处理核苷酸序列信息的简单有用的方法，装置和系统，以及简单有用的方法 检测靶核苷酸。 本发明提供了一种处理核苷酸序列信息的方法，该方法包括产生具有7-14个核苷酸并且Tm值为25至40℃的部分核苷酸序列的步骤，其中靶核苷酸或邻近 靶核苷酸位于3'或5'端3〜5个核苷酸的位置。 根据本发明的方法可用于简单且有效地制备用于检测靶核酸的含RNA探针，而无需基于研究人员的经验或猜测，并且不仅在遗传工程领域中非常有用，而且 也在医学研究领域。

    METHOD FOR PRODUCING RNA-CONTAINING PROBE FOR DETECTING A TARGET NUCLEOTIDE
    5.
    发明申请
    METHOD FOR PRODUCING RNA-CONTAINING PROBE FOR DETECTING A TARGET NUCLEOTIDE 有权
    用于生产用于检测目标核酸的含RNA探针的方法

    公开(公告)号：US20130122503A1

    公开(公告)日：2013-05-16

    申请号：US13812242

    申请日：2011-07-28

    申请人： Junko Yamamoto ,  Tomoo Inden ,  Toshiharu Ohba ,  Tooru Suzuki ,  Hiroyuki Mukai ,  Kiyozo Asada

    发明人： Junko Yamamoto ,  Tomoo Inden ,  Toshiharu Ohba ,  Tooru Suzuki ,  Hiroyuki Mukai ,  Kiyozo Asada

    IPC分类号： G06F19/12 ,  C12Q1/68

    CPC分类号： G06F19/12 ,  C12Q1/6816 ,  C12Q1/6876 ,  C12Q2527/107 ,  G06F19/20

    摘要： An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40° C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3′ or 5′ end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.

    摘要翻译： 本发明的目的在于提供一种用于检测靶核苷酸的含RNA探针的简单且有用的方法，用于处理核苷酸序列信息的简单有用的方法，装置和系统，以及简单有用的方法 检测靶核苷酸。 本发明提供了一种处理核苷酸序列信息的方法，该方法包括产生具有7-14个核苷酸并且Tm值为25至40℃的部分核苷酸序列的步骤，其中靶核苷酸或邻近 靶核苷酸位于3'或5'端3〜5个核苷酸的位置。 根据本发明的方法可用于简单且有效地制备用于检测靶核酸的含RNA探针，而无需基于研究人员的经验或猜测，并且不仅在遗传工程领域中非常有用，而且 也在医学研究领域。