-
公开(公告)号：US06730479B2
公开(公告)日：2004-05-04
申请号：US09790417
申请日：2001-02-22
申请人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
发明人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
IPC分类号： C12Q168
CPC分类号： C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6827 , Y10T436/24 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
摘要翻译： 公开了使用核酸杂交体的解聚定性和定量分析预定核酸的存在的方法。 这些过程的应用包括单核苷酸多态性的检测，单碱基变化的鉴定，物种形成，病毒载量的测定，基因分型，医学标记诊断等。
-
公开(公告)号：US06312902B1
公开(公告)日：2001-11-06
申请号：US09406065
申请日：1999-09-27
申请人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Roy Welch
发明人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Roy Welch
IPC分类号： C12Q168
CPC分类号： C12Q1/66 , C12Q1/04 , C12Q1/68 , C12Q1/6823 , C12Q1/6827 , Y10T436/24 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
摘要翻译： 公开了使用核酸杂交体的解聚定性和定量分析预定核酸的存在的方法。 这些过程的应用包括单核苷酸多态性的检测，单碱基变化的鉴定，物种形成，病毒载量的测定，基因分型，医学标记诊断等。
-
公开(公告)号：US06235480B1
公开(公告)日：2001-05-22
申请号：US09358972
申请日：1999-07-21
申请人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
发明人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
IPC分类号： C12Q168
CPC分类号： C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6827 , Y10T436/24 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
-
公开(公告)号：US20090137020A1
公开(公告)日：2009-05-28
申请号：US12356345
申请日：2009-01-20
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
IPC分类号： C12N9/00
CPC分类号： C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要： The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
摘要翻译： 本发明涉及RNA的逆转录，特别涉及耐热性DNA聚合酶的逆转录。 寻常型嗜热脂肪芽胞杆菌和嗜热脂肪芽孢杆菌在镁或锰离子存在下具有逆转录酶活性。 还提供了用于逆转录和RT-PCR的方法，组合物和试剂盒。
-
公开(公告)号：US20070082333A1
公开(公告)日：2007-04-12
申请号：US10444203
申请日：2003-05-23
申请人： Trent Gu , Fen Huang , James Hartnett
发明人： Trent Gu , Fen Huang , James Hartnett
CPC分类号： C12N9/1252 , C12N9/1276
摘要： The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译： 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地，本发明包括来自嗜热嗜热芽孢杆菌（Thermoactinomyces vulgaris）的热稳定性DNA聚合酶。 本发明还提供了在测序，逆转录和扩增反应中利用天然存在和非天然存在的寻常型寻常型DNA聚合酶的方法。
-
公开(公告)号：US06391551B1
公开(公告)日：2002-05-21
申请号：US09383316
申请日：1999-08-25
申请人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
发明人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
IPC分类号： C12Q168
CPC分类号： C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/6827 , C12Q2535/131 , C12Q2521/319 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
摘要翻译： 公开了使用核酸杂交体的解聚定性和定量分析预定的内源性核酸的存在的方法。 这些过程的应用包括单核苷酸多态性的检测，单碱基变化的鉴定，物种形成，病毒载量的测定，基因分型，医学标记诊断等。
-
公开(公告)号：US06270973B1
公开(公告)日：2001-08-07
申请号：US09406064
申请日：1999-09-27
申请人： Martin K. Lewis , Daniel Kephart , Richard Byron Rhodes , John William Shultz , Donna Leippe , Michelle Mandrekar , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Keith V. Wood , Roy Welch
发明人： Martin K. Lewis , Daniel Kephart , Richard Byron Rhodes , John William Shultz , Donna Leippe , Michelle Mandrekar , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Keith V. Wood , Roy Welch
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6823 , Y10T436/24 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of predetermined nucleic acid target sequences using a multiplex assay format. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, genotyping, medical marker diagnostics, and the like.
摘要翻译： 使用核酸杂交体的解聚来公开使用多重测定形式定性和定量分析预定核酸靶序列的存在的方法。 这些过程的应用包括单核苷酸多态性的检测，单碱基变化的鉴定，物种形成，基因分型，医学标记诊断等。
-
公开(公告)号：US07504220B2
公开(公告)日：2009-03-17
申请号：US11475663
申请日：2006-06-27
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要： The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
-
公开(公告)号：US07214522B2
公开(公告)日：2007-05-08
申请号：US10444203
申请日：2003-05-23
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
CPC分类号： C12N9/1252 , C12N9/1276
摘要： The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译： 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地，本发明包括来自嗜热嗜热芽孢杆菌（Thermoactinomyces vulgaris）的热稳定性DNA聚合酶。 本发明还提供了在测序，逆转录和扩增反应中利用天然存在和非天然存在的寻常型寻常型DNA聚合酶的方法。
-
公开(公告)号：US06632645B1
公开(公告)日：2003-10-14
申请号：US09517439
申请日：2000-03-02
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
IPC分类号： C12N912
CPC分类号： C12N9/1252 , C12N9/1276
摘要： The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译： 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地，本发明包括来自超嗜热真细菌物种的热稳定DNA聚合酶。 本发明还提供了利用天然存在和非天然存在形式的
T的方法。 寻常型 测序，逆转录和扩增反应中的DNA聚合酶。
-
-
-
-
-
-
-
-
-
搜索结果
18 条记录 (耗时 0.057 秒)