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公开(公告)号：US20060240468A1
公开(公告)日：2006-10-26
申请号：US11475663
申请日：2006-06-27
申请人： Trent Gu , Fen Huang , James Hartnett
发明人： Trent Gu , Fen Huang , James Hartnett
CPC分类号： C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要： The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
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公开(公告)号：US20070172879A1
公开(公告)日：2007-07-26
申请号：US11725784
申请日：2007-03-19
申请人： Trent Gu , Fen Huang , James Hartnett
发明人： Trent Gu , Fen Huang , James Hartnett
CPC分类号： C12N9/1252 , C12N9/1276
摘要： The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译： 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地，本发明包括来自嗜热嗜热芽孢杆菌（Thermoactinomyces vulgaris）的热稳定性DNA聚合酶。 本发明还提供了在测序，逆转录和扩增反应中利用天然存在和非天然存在的寻常型寻常型DNA聚合酶的方法。
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公开(公告)号：US20070082333A1
公开(公告)日：2007-04-12
申请号：US10444203
申请日：2003-05-23
申请人： Trent Gu , Fen Huang , James Hartnett
发明人： Trent Gu , Fen Huang , James Hartnett
CPC分类号： C12N9/1252 , C12N9/1276
摘要： The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译： 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地，本发明包括来自嗜热嗜热芽孢杆菌（Thermoactinomyces vulgaris）的热稳定性DNA聚合酶。 本发明还提供了在测序，逆转录和扩增反应中利用天然存在和非天然存在的寻常型寻常型DNA聚合酶的方法。
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4.发明授权公开(公告)号：US07094539B2
公开(公告)日：2006-08-22
申请号：US10178673
申请日：2002-06-24
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要： The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
摘要翻译： 本发明涉及RNA的逆转录，特别涉及耐热性DNA聚合酶的逆转录。 寻常型嗜热脂肪芽胞杆菌和嗜热脂肪芽孢杆菌在镁或锰离子存在下具有逆转录酶活性。 还提供了用于逆转录和RT-PCR的方法，组合物和试剂盒。
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公开(公告)号：US06436677B1
公开(公告)日：2002-08-20
申请号：US09517871
申请日：2000-03-02
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
IPC分类号： C12Q168
CPC分类号： C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要： The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
摘要翻译： 本发明涉及RNA的逆转录，特别涉及耐热性DNA聚合酶的逆转录。 寻常型嗜热脂肪芽胞杆菌和嗜热脂肪芽孢杆菌在镁或锰离子存在下具有逆转录酶活性。 还提供了用于逆转录和RT-PCR的方法，组合物和试剂盒。
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公开(公告)号：US20090137020A1
公开(公告)日：2009-05-28
申请号：US12356345
申请日：2009-01-20
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
IPC分类号： C12N9/00
CPC分类号： C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要： The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
摘要翻译： 本发明涉及RNA的逆转录，特别涉及耐热性DNA聚合酶的逆转录。 寻常型嗜热脂肪芽胞杆菌和嗜热脂肪芽孢杆菌在镁或锰离子存在下具有逆转录酶活性。 还提供了用于逆转录和RT-PCR的方法，组合物和试剂盒。
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公开(公告)号：US07504220B2
公开(公告)日：2009-03-17
申请号：US11475663
申请日：2006-06-27
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要： The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
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公开(公告)号：US07214522B2
公开(公告)日：2007-05-08
申请号：US10444203
申请日：2003-05-23
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
CPC分类号： C12N9/1252 , C12N9/1276
摘要： The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译： 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地，本发明包括来自嗜热嗜热芽孢杆菌（Thermoactinomyces vulgaris）的热稳定性DNA聚合酶。 本发明还提供了在测序，逆转录和扩增反应中利用天然存在和非天然存在的寻常型寻常型DNA聚合酶的方法。
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公开(公告)号：US06632645B1
公开(公告)日：2003-10-14
申请号：US09517439
申请日：2000-03-02
申请人： Trent Gu , Fen Huang , James Robert Hartnett
发明人： Trent Gu , Fen Huang , James Robert Hartnett
IPC分类号： C12N912
CPC分类号： C12N9/1252 , C12N9/1276
摘要： The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
摘要翻译： 本发明提供包含衍生自嗜热细菌的热稳定DNA聚合酶的组合物。 特别地，本发明包括来自超嗜热真细菌物种的热稳定DNA聚合酶。 本发明还提供了利用天然存在和非天然存在形式的
T的方法。 寻常型 测序，逆转录和扩增反应中的DNA聚合酶。 -
公开(公告)号：US09816127B2
公开(公告)日：2017-11-14
申请号：US13222697
申请日：2011-08-31
申请人： Fen Huang , Dieter Klaubert , John Shultz , Wenhui Zhou
发明人： Fen Huang , Dieter Klaubert , John Shultz , Wenhui Zhou
CPC分类号： C12Q1/48 , G01N21/763 , G01N2333/91177
摘要： The present invention provides an assay for detection of oxidized glutathione (GSSG).
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