Method of reverse transcription
    9.
    发明授权
    Method of reverse transcription 失效
    逆转录方法

    公开(公告)号:US06436677B1

    公开(公告)日:2002-08-20

    申请号:US09517871

    申请日:2000-03-02

    IPC分类号: C12Q168

    摘要: The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.

    摘要翻译: 本发明涉及RNA的逆转录,特别涉及耐热性DNA聚合酶的逆转录。 寻常型嗜热脂肪芽胞杆菌和嗜热脂肪芽孢杆菌在镁或锰离子存在下具有逆转录酶活性。 还提供了用于逆转录和RT-PCR的方法,组合物和试剂盒。