公开(公告)号：US07262173B2

公开(公告)日：2007-08-28

申请号：US10075994

申请日：2002-02-15

Applicant: Usha Kasid ,  Prafulla Gokhale ,  Jin Pei ,  Rajshree Mewani ,  Imran Ahmad ,  Anatoly Dritschilo ,  Aquilur Rahman

Inventor： Usha Kasid ,  Prafulla Gokhale ,  Jin Pei ,  Rajshree Mewani ,  Imran Ahmad ,  Anatoly Dritschilo ,  Aquilur Rahman

IPC: A01N43/04 ,  A01N61/00 ,  C12N15/88 ,  C12Q1/68 ,  C07H21/04

CPC classification number: C12N15/1135 ,  A61K9/1272 ,  A61K31/7088 ,  A61K31/7125 ,  A61K38/00 ,  A61K41/00 ,  A61K45/06 ,  C07H21/00 ,  C12N2310/315 ,  C12N2310/341 ,  C12N2310/345 ,  A61K2300/00

Abstract: The invention relates to the use of oligonucleotide containing cationic liposomal formulations to enhance the efficacy of chemotherapy and/or radiotherapy, particularly as a means to sensitize cancerous tumor tissues to the efficiencies of chemotherapy. This is particularly advantageous in the context of treating raf expressing tumors such as breast, lung, pancreatic and prostate tumors.

Abstract translation: 本发明涉及使用含有阳离子脂质体制剂的寡核苷酸来提高化学疗法和/或放射疗法的功效，特别是作为使癌性肿瘤组织对化疗效率敏感的手段。 这在治疗raf表达肿瘤如乳腺癌，肺癌，胰腺癌和前列腺肿瘤的情况下特别有利。

公开(公告)号：US6126965A

公开(公告)日：2000-10-03

申请号：US957327

申请日：1997-10-24

Applicant: Usha Kasid ,  Prafulla Gokhale ,  Anatoly Dritschilo ,  Aquilur Rahman

Inventor： Usha Kasid ,  Prafulla Gokhale ,  Anatoly Dritschilo ,  Aquilur Rahman

IPC: A61K9/127 ,  A61K38/00 ,  C07H21/00 ,  C12N15/113 ,  C07H21/04

CPC classification number: C12N15/1135 ,  A61K9/127 ,  A61K9/1272 ,  C07H21/00 ,  C12N15/88 ,  A61K38/00 ,  A61K9/0019 ,  C12N2310/315 ,  C12N2310/345 ,  Y10S436/829

Abstract: It is possible to radiosensitize tumor cells by administration of compositions containing the Human antisense c-raf-1 oligodeoxyribonucleotide (ODN/oligo) sequence: 5'-GTGCTCCATTGATGC-3' (seq. #1) wherein only the end bases are phosphorylated is a preferred embodiment. Antisense sequences of up to 40 bases which containing this sequence may be used in accord with the teachings of this disclosure. Compositions comprising a cationic liposome of dimethyldioctadecyl ammonium bromide, phosphatidylcholine and cholesterol may be used as a carrier system. The liposomes provide a new carrier system that is particularly useful for administration of sequences for therapy.

公开(公告)号：US20110104252A1

公开(公告)日：2011-05-05

申请号：US12858360

申请日：2010-08-17

Applicant: USHA KASID ,  Deepak Kumar ,  Prafulla Gokhale ,  Imran Ahmad

Inventor： USHA KASID ,  Deepak Kumar ,  Prafulla Gokhale ,  Imran Ahmad

IPC: A61K9/127 ,  A61K31/7088 ,  A61K39/395 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  A61P35/00 ,  A61P35/02

CPC classification number: C07K14/4747 ,  A61K38/1709 ,  A61K2039/505

Abstract: A gene that is a positive mediator of tumor growth and metastasis in certain cancer types is provided. This gene and corresponding polypeptide have diagnostic and therapeutic application for detecting and treating cancers that involve expression of SCC-S2 such as renal, ovarian, head and neck, breast, prostate, brain, chronic myelogenous leukemia, lung, lymphoblastic leukemia, and colorectal adenocarcinoma cells.

Abstract translation: 提供了一种在某些癌症类型中是肿瘤生长和转移的阳性介质的基因。 该基因和相应的多肽具有检测和治疗涉及SCC-S2表达的癌症的诊断和治疗应用，例如肾，卵巢，头颈部，乳腺，前列腺，脑，慢性骨髓性白血病，肺，淋巴细胞白血病和结肠直肠腺癌 细胞。

公开(公告)号：US07244565B2

公开(公告)日：2007-07-17

申请号：US10411930

申请日：2003-04-10

Applicant: Usha Kasid ,  Isamu Sakabe ,  Imran Ahmad

Inventor： Usha Kasid ,  Isamu Sakabe ,  Imran Ahmad

IPC: C07H21/02

CPC classification number: C07K14/47 ,  A61K38/00 ,  A61K2039/505 ,  C07H21/04

Abstract: The invention provides a SHINC-3 polynucleotide, which can be a nucleic acid encoding all or a portion of a SHINC-3 protein, or a complementary polynucleotide or antisense polynucleotide. In another aspect, the invention provides a SHINC-3 polypeptide, which can be a full-length SHINC-3 protein or a fragment thereof or an analog or homolog thereof. Desirably, the SHINC-3 polypeptide modulates apoptosis. In another aspect, the invention provides an antibody that specifically binds a SHINC-3 polypeptide.In another aspect, the invention provides diagnostic methods. For example, the method affords a method for identifying compounds that modulate apoptosis. In another aspect, the invention provides a method for detecting or evaluating the prognosis of a cancer. In another aspect, the invention provides diagnostic compositions for detection of cancer.In another aspect, the invention provides a method of modulating apoptosis or invention or preventing a cancer, tumor growth and/or metastasis by administration of an agent that modulates the expression and/or activity of SHINC-3.In another aspect, the invention provides formulations of SHINC-3 polynucleotides or proteins. Preferably, such compositions will comprise liposomal formulations.

Abstract translation: 本发明提供了SHINC-3多核苷酸，其可以是编码SHINC-3蛋白的全部或部分的核酸或互补多核苷酸或反义多核苷酸。 另一方面，本发明提供了SHINC-3多肽，其可以是全长SHINC-3蛋白或其片段或其类似物或同系物。 理想地，SHINC-3多肽调节凋亡。 另一方面，本发明提供了特异性结合SHINC-3多肽的抗体。 另一方面，本发明提供诊断方法。 例如，该方法提供了鉴定调节凋亡的化合物的方法。 另一方面，本发明提供了用于检测或评价癌症预后的方法。 另一方面，本发明提供了用于检测癌症的诊断组合物。 另一方面，本发明提供调节凋亡或发明的方法或通过施用调节SHINC-3的表达和/或活性的试剂来预防癌症，肿瘤生长和/或转移。 另一方面，本发明提供SHINC-3多核苷酸或蛋白质的制剂。 优选地，这样的组合物将包含脂质体制剂。

公开(公告)号：US20070087992A1

公开(公告)日：2007-04-19

申请号：US11600437

申请日：2006-11-16

Applicant: Usha Kasid ,  Deepak Kumar ,  Prafulla Gokhale ,  Imran Ahmad

Inventor： Usha Kasid ,  Deepak Kumar ,  Prafulla Gokhale ,  Imran Ahmad

IPC: A61K48/00 ,  A61K39/395

CPC classification number: C07K14/4747 ,  A61K38/1709 ,  A61K2039/505

Abstract: A gene that is a positive mediator of tumor growth and metastasis in certain cancer types is provided. This gene and corresponding polypeptide have diagnostic and therapeutic application for detecting and treating cancers that involve expression of SCC-S2 such as renal, ovarian, head and neck, breast, prostate, brain, chronic myelogenous leukemia, lung, lymphoblastic leukemia, and colorectal adenocarcinoma cells.

Abstract translation: 提供了一种在某些癌症类型中是肿瘤生长和转移的阳性介质的基因。 该基因和相应的多肽具有检测和治疗涉及SCC-S2表达的癌症的诊断和治疗应用，例如肾，卵巢，头颈部，乳腺，前列腺，脑，慢性骨髓性白血病，肺，淋巴细胞白血病和结肠直肠腺癌 细胞。

公开(公告)号：US07138512B2

公开(公告)日：2006-11-21

申请号：US10411931

申请日：2003-04-10

Applicant: Usha Kasid ,  Isamu Sakabe ,  Imran Ahmad

Inventor： Usha Kasid ,  Isamu Sakabe ,  Imran Ahmad

IPC: C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/12 ,  C12N15/63

CPC classification number: C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/505

Abstract: The invention provides a SHINC-2 polynucleotide, which can be a nucleic acid encoding all or a portion of a SHINC-2 protein, or a complementary polynucleotide or antisense polynucleotide. The invention provides a SHINC-2 polypeptide, which can be a full-length SHINC-2 protein or a fragment thereof or an analog or homolog thereof. Desirably, the SHINC-2 polypeptide modulates apoptosis. The invention provides an antibody that specifically binds a SHINC-2 polypeptide. The invention provides diagnostic methods. For example, the invention affords a method for identifying compounds that modulate apoptosis. The invention provides a method for detecting or evaluating the prognosis of a cancer. The invention provides diagnostic compositions for detection of cancer. The invention provides a method of modulating apoptosis or treating or preventing a cancer, tumor growth and/or metastasis by administration of an agent that modulates the expression and/or activity of SHINC-2. The invention provides formulations of SHINC-2 polynucleotides or proteins. Preferably, such compositions will comprise liposomal formulations.

Abstract translation: 本发明提供了SHINC-2多核苷酸，其可以是编码SHINC-2蛋白的全部或部分的核酸或互补多核苷酸或反义多核苷酸。 本发明提供了SHINC-2多肽，其可以是全长SHINC-2蛋白或其片段或其类似物或同系物。 理想地，SHINC-2多肽调节凋亡。 本发明提供了特异性结合SHINC-2多肽的抗体。 本发明提供诊断方法。 例如，本发明提供了鉴定调节凋亡的化合物的方法。 本发明提供了一种检测或评价癌症预后的方法。 本发明提供了用于癌症检测的诊断组合物。 本发明提供调节凋亡或通过施用调节SHINC-2的表达和/或活性的试剂来治疗或预防癌症，肿瘤生长和/或转移的方法。 本发明提供SHINC-2多核苷酸或蛋白质的制剂。 优选地，这样的组合物将包含脂质体制剂。

公开(公告)号：US06333314B1

公开(公告)日：2001-12-25

申请号：US09482084

申请日：2000-01-13

Applicant: Usha Kasid ,  Prafulla Gokhale ,  Anatoly Dritschilo ,  Aquilur Rahman

Inventor： Usha Kasid ,  Prafulla Gokhale ,  Anatoly Dritschilo ,  Aquilur Rahman

IPC: A01N4304

CPC classification number: C12N15/1135 ,  A61K9/0019 ,  A61K9/127 ,  A61K9/1272 ,  A61K38/00 ,  C07H21/00 ,  C12N15/88 ,  C12N2310/315 ,  C12N2310/345 ,  Y10S436/829

Abstract: It is possible to radiosensitize tumor cells by administration of compositions containing the Human antisense c-raf-1 oligodeoxyribonucleotide (ODN/oligo) sequence: 5′-GTGCTCCATTGATGC-3′ (seq. ID No. 1) wherein only the end bases are phosphorylated is a preferred embodiment. Antisense sequences of up to 40 bases which containing this sequence may be used in accord with the teachings of this disclosure. Compositions comprising a cationic liposome of dimethyldioctadecyl ammonium bromide, phosphatidylcholine and cholesterol may be used as a carrier system. The liposomes provide a new carrier system that is particularly useful for administration of sequences for therapy.

Abstract translation: 通过给予含有人反义c-raf-1寡脱氧核糖核苷酸（ODN / oligo）序列的组合物：5'-GTGCTCCATTGATGC-3'（序列号1），其中只有末端碱基被磷酸化，可以对肿瘤细胞进行放射增敏 是优选实施例。 可以根据本公开的教导使用含有该序列的多达40个碱基的反义序列。 可以使用包含二甲基十二烷基溴化铵的阳离子脂质体，磷脂酰胆碱和胆固醇的组合物作为载体体系。 脂质体提供了一种新的载体系统，其特别可用于给予治疗的序列。

公开(公告)号：US20100041142A1

公开(公告)日：2010-02-18

申请号：US12467802

申请日：2009-05-18

Applicant: Usha Kasid ,  Deepak Kumar ,  Prafulla Gokhale ,  Imran Ahmad

Inventor： Usha Kasid ,  Deepak Kumar ,  Prafulla Gokhale ,  Imran Ahmad

IPC: C12N5/02

CPC classification number: C07K14/4747 ,  A61K38/1709 ,  A61K2039/505

Abstract: A gene that is a positive mediator of tumor growth and metastasis in certain cancer types is provided. This gene and corresponding polypeptide have diagnostic and therapeutic application for detecting and treating cancers that involve expression of SCC-S2 such as renal, ovarian, head and neck, breast, prostate, brain, chronic myelogenous leukemia, lung, lymphoblastic leukemia, and colorectal adenocarcinoma cells.

Abstract translation: 提供了一种在某些癌症类型中是肿瘤生长和转移的阳性介质的基因。 该基因和相应的多肽具有检测和治疗涉及SCC-S2表达的癌症的诊断和治疗应用，例如肾，卵巢，头颈部，乳腺，前列腺，脑，慢性骨髓性白血病，肺，淋巴细胞白血病和结肠直肠腺癌 细胞。

公开(公告)号：US07442520B2

公开(公告)日：2008-10-28

申请号：US10679561

申请日：2003-10-06

Applicant: Usha Kasid ,  Prafulla Gokhale ,  Deepak Kumar ,  Howard Boudreau ,  Imran Ahmad ,  Anatoly Dritschilo ,  Aquilur Rahman

Inventor： Usha Kasid ,  Prafulla Gokhale ,  Deepak Kumar ,  Howard Boudreau ,  Imran Ahmad ,  Anatoly Dritschilo ,  Aquilur Rahman

IPC: C12Q1/68 ,  C12P19/34 ,  C12P21/06 ,  C07H21/02 ,  C07H21/04

CPC classification number: C07K14/82 ,  A61K38/00 ,  C12Q1/6886 ,  C12Q2600/136 ,  G01N33/574

Abstract: A gene that is a modulator of tumor growth and metastasis in certain cancer types is provided. This gene and corresponding polypeptide have diagnostic and therapeutic application for detecting and treating cancers that involve expression of BRCC-3 such as breast cancer and lung cancer.

Abstract translation: 提供了一种在某些癌症类型中是肿瘤生长和转移的调节剂的基因。 该基因和相应的多肽具有检测和治疗涉及乳腺癌和肺癌等BRCC-3表达的癌症的诊断和治疗应用。

公开(公告)号：US07351811B2

公开(公告)日：2008-04-01

申请号：US10679580

申请日：2003-10-06

Applicant: Usha Kasid ,  Deepak Kumar ,  Imran Ahmad

Inventor： Usha Kasid ,  Deepak Kumar ,  Imran Ahmad

IPC: C07H21/04

CPC classification number: C07K14/82 ,  A61K38/00 ,  G01N33/574

Abstract: A gene that is a modulator of tumor growth and metastasis in certain cancer types is provided. SCC-112 (about 150 kDa) and/or a mutant form of SCC-112 (about 65 kDa) is a tumor suppressor molecule. This gene and corresponding polypeptide have diagnostic and therapeutic application for detecting and treating cancers that involve expression of SCC-112 such as breast and kidney cancers.

Abstract translation: 提供了一种在某些癌症类型中是肿瘤生长和转移的调节剂的基因。 SCC-112（约150kDa）和/或突变形式的SCC-112（约65kDa）是肿瘤抑制分子。 该基因和相应的多肽具有用于检测和治疗涉及表达SCC-112（例如乳腺和肾癌）的癌症的诊断和治疗应用。