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公开(公告)号：US08003358B2
公开(公告)日：2011-08-23
申请号：US11990115
申请日：2006-08-02
申请人： Jun Wang , Waikei Tsang , Hongkin Yap , Junmin Chen , Yaulung Siu , Supyin Tsang
发明人： Jun Wang , Waikei Tsang , Hongkin Yap , Junmin Chen , Yaulung Siu , Supyin Tsang
CPC分类号： C12P35/02 , C12N9/0024 , C12P35/06
摘要： The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast Trigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, β-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity.
摘要翻译： 本发明公开了一种用于从头孢菌素C制备7-氨基头孢烷酸的两步酶方法，其中使用D-氨基酸氧化酶是酵母Trigonopsis variabilis的纯化D-氨基酸氧化酶突变体，比活性比其活性高105％ 的母体D-氨基酸氧化酶。 该方法不需要加入现有技术中常用的过氧化氢，β-内酰胺酶抑制剂，过氧化氢酶抑制剂，过氧化氢酶等。 该方法的生产率可以达到93％以上。 因此，该方法简单，成本低，生产率高。
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公开(公告)号：US20100112633A1
公开(公告)日：2010-05-06
申请号：US11990115
申请日：2006-08-02
申请人： Jun Wang , Waikei Tsang , Hongkin Yap , Junmin Chen , Yaulung Siu , Supyin Tsang
发明人： Jun Wang , Waikei Tsang , Hongkin Yap , Junmin Chen , Yaulung Siu , Supyin Tsang
IPC分类号： C12P35/00
CPC分类号： C12P35/02 , C12N9/0024 , C12P35/06
摘要： The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast Trigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, β-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity.
摘要翻译： 本发明公开了一种用于从头孢菌素C制备7-氨基头孢烷酸的两步酶方法，其中使用D-氨基酸氧化酶是酵母Trigonopsis variabilis的纯化D-氨基酸氧化酶突变体，比活性比其活性高105％ 的母体D-氨基酸氧化酶。 该方法不需要加入现有技术中常用的过氧化氢，β-内酰胺酶抑制剂，过氧化氢酶抑制剂，过氧化氢酶等。 该方法的生产率可以达到93％以上。 因此，该方法简单，成本低，生产率高。
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公开(公告)号：US07517677B2
公开(公告)日：2009-04-14
申请号：US11547639
申请日：2004-04-12
申请人： Jun Wang , Hongkin Yap , Waikei Tsang , Yuksun Lui , Yaulung Philip Siu , Supyin Tsang , Minghon Yau
发明人： Jun Wang , Hongkin Yap , Waikei Tsang , Yuksun Lui , Yaulung Philip Siu , Supyin Tsang , Minghon Yau
CPC分类号： C12N9/0024
摘要： The present invention discloses two novel D-amino acid oxidases, whose activities of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid are at least 25% higher than that of wild-type Trigonopsis variabilis D-amino acid oxidase and the encoding DNA sequences thereof.
摘要翻译： 本发明公开了两种新型D-氨基酸氧化酶，其转化头孢菌素C至戊二酰-7-氨基头孢菌酸的活性比野生型变异型D-氨基酸氧化酶高出至少25％，编码其DNA序列 。
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公开(公告)号：US20080009052A1
公开(公告)日：2008-01-10
申请号：US11547639
申请日：2004-04-12
申请人： Jun Wang , Hongkin Yap , Waikei Tsang , Yuksun Lui , Yaulung Siu , Supyin Tsang , Minghon Yau
发明人： Jun Wang , Hongkin Yap , Waikei Tsang , Yuksun Lui , Yaulung Siu , Supyin Tsang , Minghon Yau
CPC分类号： C12N9/0024
摘要： The present invention discloses two novel D-amino acid oxidases, whose activities of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid are at least 25% higher than that of wild-type Trigonopsis variabilis D-amino acid oxidase and the encoding DNA sequences thereof.
摘要翻译： 本发明公开了两种新型D-氨基酸氧化酶，其转化头孢菌素C至戊二酰-7-氨基头孢菌酸的活性比野生型变异型D-氨基酸氧化酶高出至少25％，编码其DNA序列 。
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