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    Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors
    5.
    发明授权
    Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors 有权
    1,4-丁二醇及其前体的生物合成的组合物和方法

    公开(公告)号：US08067214B2

    公开(公告)日：2011-11-29

    申请号：US12049256

    申请日：2008-03-14

    申请人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    发明人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    IPC分类号： C12P7/18 ,  C12N1/21

    CPC分类号： C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

    摘要： The invention provides a non-naturally occurring microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (1,4-BDO) biosynthetic pathways. The pathways include exogenous nucleic acids encoding a) an α-ketoglutarate decarboxylase; b) a 4-hydroxybutanoate dehydrogenase; c) a 4-hydroxybutyryl-CoA:acetyl-CoA transferase or a butyrate kinase and a phosphotransbutyrylase; d) an aldehyde dehydrogenase, and e) an alcohol dehydrogenase, wherein the exogenous nucleic acids are expressed in sufficient amounts to produce 1,4-butanediol (1,4-BDO). Also provide is a method for the production of 1,4-BDO. The method includes culturing the non-naturally occurring microbial organism having 4-HB and 1,4-BDO biosynthetic pathways substantially anaerobic conditions for a sufficient period of time to produce 1,4-BDO.

    摘要翻译： 本发明提供了具有4-羟基丁酸（4-HB）和1,4-丁二醇（1,4-BDO）生物合成途径的非天然存在的微生物生物。 途径包括编码a）α-酮戊二酸脱羧酶的外源核酸; b）4-羟基丁酸脱氢酶; c）4-羟基丁酰-CoA：乙酰-CoA转移酶或丁酸酯激酶和磷酸转磷酸化酶; d）醛脱氢酶，和e）醇脱氢酶，其中外源核酸以足够的量表达以产生1,4-丁二醇（1,4-BDO）。 还提供了用于生产1,4-BDO的方法。 该方法包括培养具有4-HB和1,4-BDO生物合成途径的非天然存在的微生物生物合成途径，基本上是厌氧条件足够的时间以产生1,4-BDO。

    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS
    8.
    发明申请
    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 有权
    1,4-丁二醇及其前体的生物合成的组合物和方法

    公开(公告)号：US20130196397A1

    公开(公告)日：2013-08-01

    申请号：US13717350

    申请日：2012-12-17

    申请人： Mark J. BURK ,  Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Wei NIU

    发明人： Mark J. BURK ,  Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Wei NIU

    IPC分类号： C12P7/42 ,  C12P7/18

    CPC分类号： C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

    摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

    摘要翻译： 本发明提供了一种非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）生物合成途径的微生物生物，其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛 脱氢酶或α-酮戊二酸脱羧酶，其中外源核酸以足够的量表达以产生单体4-羟基丁酸（4-HB）。 还提供了非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸 ，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶，4-羟基丁酸酯：CoA转移酶，4-丁酸酯激酶，磷酸转移酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，其中外源核酸为 以足够的量表示产生1,4-丁二醇（BDO）。 另外提供了用于生产4-HB和BDO的方法。

    MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL, 4-HYDROXYBUTANAL, 4-HYDROXYBUTYRYL-COA, PUTRESCINE AND RELATED COMPOUNDS, AND METHODS RELATED THERETO
    9.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL, 4-HYDROXYBUTANAL, 4-HYDROXYBUTYRYL-COA, PUTRESCINE AND RELATED COMPOUNDS, AND METHODS RELATED THERETO 审中-公开
    用于生产1,4-丁二醇，4-羟基吡啶，4-羟基丁酰氯，聚氨酯和相关化合物的微生物及其相关方法

    公开(公告)号：US20130189751A1

    公开(公告)日：2013-07-25

    申请号：US13737866

    申请日：2013-01-09

    申请人： Robert HASELBECK ,  John D. TRAWICK ,  Wei NIU ,  Anthony P. BURGARD

    发明人： Robert HASELBECK ,  John D. TRAWICK ,  Wei NIU ,  Anthony P. BURGARD

    IPC分类号： C12P7/24

    CPC分类号： C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P13/001 ,  C12P19/40

    摘要： The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

    摘要翻译： 本发明提供了包含1,4-丁二醇（BDO），4-羟基丁酰辅酶A，4-羟基丁醛或腐胺途径的非天然存在的微生物，其包含至少一种编码BDO，4-羟基丁酰辅酶A， 表达量足以产生BDO，4-羟基丁酰辅酶A，4-羟基丁醛或腐胺的β-羟基丁酸或腐胺途径酶，并进一步优化BDO的表达。 本发明另外提供了使用这些微生物生产BDO，4-羟基丁酰辅酶A，4-羟基丁醛或腐胺的方法。