公开(公告)号：US07572632B2

公开(公告)日：2009-08-11

申请号：US10685802

申请日：2003-10-16

申请人： Richard Fike ,  William Whitford ,  William Biddle

发明人： Richard Fike ,  William Whitford ,  William Biddle

IPC分类号： C12N6/00 ,  C12N1/00 ,  C12N1/20 ,  C12N5/00

CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

摘要： Methods and compositions involving nutritive media, media supplements, media subgroup and buffer formulations are provided. Powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are also disclosed. Methods of producing these media, media supplements, media subgroups and buffer formulations as well as kits and methods for cultivation of procaryotic and eukaryotic cells (including human cells) are also provided. Also disclosed are methods of producing sterile powdered media, media supplements, powdered growth factors, media subgroup and buffer formulations. Methods of sterilization include gamma irradiation. Methods for producing dry cell powders where spray-drying a cell suspension may be used are disclosed. Cells, media, media supplements, media subgroups and buffer powders produced by these methods are also disclosed.

摘要翻译： 提供了涉及营养介质，培养基补充剂，培养基亚组和缓冲液制剂的方法和组合物。 还公开了在用溶剂重构时产生特定离子和pH条件的粉末缓冲剂制剂。 还提供了生产这些培养基，培养基，培养基和缓冲液制剂的方法以及用于培养原核和真核细胞（包括人细胞）的试剂盒和方法。 还公开了生产无菌粉末培养基，培养基补充剂，粉末生长因子，培养基亚组和缓冲液制剂的方法。 灭菌方法包括γ照射。 公开了可以使用喷雾干燥细胞悬浮液的干细胞粉末的制造方法。 还公开了通过这些方法生产的细胞，培养基，培养基，培养基和缓冲粉。

公开(公告)号：US20080019883A1

公开(公告)日：2008-01-24

申请号：US11669827

申请日：2007-01-31

申请人： Richard Fike ,  William Whitford ,  Richard Hassett ,  William Biddle ,  Jeffrey Biddle ,  Laurel Biddle ,  Christine Biddle ,  Robert Radominski ,  Barbara Dadey

发明人： Richard Fike ,  William Whitford ,  Richard Hassett ,  William Biddle ,  Jeffrey Biddle ,  Laurel Biddle ,  Christine Biddle ,  Robert Radominski ,  Barbara Dadey

IPC分类号： B01J8/18 ,  C12N5/00

CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

公开(公告)号：US20060003448A1

公开(公告)日：2006-01-05

申请号：US11024053

申请日：2004-12-29

申请人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle ,  Richard Hassett ,  Barbara Dadey

发明人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle ,  Richard Hassett ,  Barbara Dadey

IPC分类号： C12N5/02

CPC分类号： C12N5/0018

摘要： The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

公开(公告)号：US06383810B2

公开(公告)日：2002-05-07

申请号：US09023790

申请日：1998-02-13

申请人： Richard Fike ,  William Whitford ,  William Biddle

发明人： Richard Fike ,  William Whitford ,  William Biddle

IPC分类号： C12N500

CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

摘要： The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation. The invention also relates to methods for producing dry cell powders, comprising spray-drying a cell suspension. The invention also relates to cell, media, media supplement, media subgroup and buffer powders produced by these methods.

摘要翻译： 本发明一般涉及营养培养基，培养基，培养基和缓冲液制剂。 具体地说，本发明提供粉末营养培养基，中等添加剂和中等亚组分制剂，特别是细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养物质的细胞培养基 促进细胞体外培养的因素。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明特别涉及这些培养基，培养基，培养基和缓冲液制剂的生产方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞的试剂盒和方法（包括 人类细胞），使用这些干粉营养介质，培养基补充剂，培养基亚组和缓冲液制剂。 本发明还涉及生产无菌粉末培养基，培养基补充剂（特别是粉末状血清如粉末状FBS，粉状转铁蛋白，粉末状胰岛素，粉末状器官提取物（如牛脑或垂体提取物）），粉末生长因子（如EGF， FGF等）等），介质亚组和缓冲液制剂。 在特别优选的方面，本发明涉及这样的方法，其中灭菌是通过γ照射来完成的。 本发明还涉及生产干细胞粉末的方法，包括喷雾干燥细胞悬浮液。 本发明还涉及通过这些方法制备的细胞，培养基，培养基，培养基和缓冲粉。

公开(公告)号：US20130109094A1

公开(公告)日：2013-05-02

申请号：US13614588

申请日：2012-09-13

申请人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

发明人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

IPC分类号： C12N5/00

CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。

公开(公告)号：US20080311660A1

公开(公告)日：2008-12-18

申请号：US11969338

申请日：2008-01-04

申请人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

发明人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

IPC分类号： C12N5/06

CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。

公开(公告)号：US20060003447A1

公开(公告)日：2006-01-05

申请号：US11024051

申请日：2004-12-29

申请人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle

发明人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle

IPC分类号： C12N5/02

CPC分类号： C12N5/0018 ,  C12N2500/60

摘要： The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

公开(公告)号：US20080261308A1

公开(公告)日：2008-10-23

申请号：US12018035

申请日：2008-01-22

申请人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

发明人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

IPC分类号： C12N5/00

CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。

公开(公告)号：US20130065300A1

公开(公告)日：2013-03-14

申请号：US13610370

申请日：2012-09-11

申请人： Richard FIKE ,  William Whitford ,  William Biddle

发明人： Richard FIKE ,  William Whitford ,  William Biddle

IPC分类号： C12N5/07 ,  A61L2/08 ,  C12N5/12 ,  C12N1/16 ,  C12N5/04

CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

摘要： The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, powdered nutritive medium, supplement, subgroup formulations, cell culture media comprising all of the necessary nutritive factors for in vitro cell cultivation, buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are provided. Particularly, methods of production of these media, supplement, subgroup, buffer formulations and kits, and methods for the cultivation of prokaryotic and eukaryotic cells using these dry powdered nutritive media, supplement, subgroup and buffer formulations are provided. Methods of producing sterile, powdered media or supplement (e.g., powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts, powdered growth factors), media subgroup and buffer formulations by gamma irradiation are provided. Methods for producing dry cell powders, comprising spray-drying a cell suspension, and cells, media, media supplement, media subgroup and buffer powders produced by these methods are provided.

摘要翻译： 本发明一般涉及营养培养基，培养基，培养基和缓冲液制剂。 具体地，提供粉状营养培养基，补充剂，亚组制剂，包含用于体外细胞培养的所有必需营养因子的细胞培养基，在用溶剂重构时产生特定的离子和pH条件的缓冲剂制剂。 特别地，提供了这些培养基，补充剂，亚组，缓冲剂配方和试剂盒的生产方法，以及使用这些干粉状营养培养基，补充剂，亚组和缓冲液制剂培养原核和真核细胞的方法。 提供了生产无菌粉末状介质或补充剂（例如，粉末状FBS，粉状转铁蛋白，粉末状胰岛素，粉末状器官提取物，粉末生长因子），培养基亚组和通过γ辐射的缓冲液制剂的方法。 提供了制备干细胞粉末的方法，包括喷雾干燥细胞悬浮液，以及通过这些方法生产的细胞，培养基，培养基，培养基和缓冲粉末。

公开(公告)号：US20120276630A1

公开(公告)日：2012-11-01

申请号：US13452498

申请日：2012-04-20

申请人： Richard FIKE ,  William Whitford ,  William Biddle

发明人： Richard FIKE ,  William Whitford ,  William Biddle

IPC分类号： C12N5/02 ,  C12N1/16 ,  C12N5/07 ,  C12N5/04

CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

摘要： The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, powdered nutritive medium, supplement, subgroup formulations, cell culture media comprising all of the necessary nutritive factors for in vitro cell cultivation, buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are provided. Particularly, methods of production of these media, supplement, subgroup, buffer formulations and kits, and methods for the cultivation of prokaryotic and eukaryotic cells using these dry powdered nutritive media, supplement, subgroup and buffer formulations are provided. Methods of producing sterile, powdered media or supplement (e.g., powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts, powdered growth factors), media subgroup and buffer formulations by gamma irradiation are provided. Methods for producing dry cell powders, comprising spray-drying a cell suspension, and cells, media, media supplement, media subgroup and buffer powders produced by these methods are provided.

摘要翻译： 本发明一般涉及营养培养基，培养基，培养基和缓冲液制剂。 具体地，提供粉状营养培养基，补充剂，亚组制剂，包含用于体外细胞培养的所有必需营养因子的细胞培养基，在用溶剂重构时产生特定的离子和pH条件的缓冲剂制剂。 特别地，提供了这些培养基，补充剂，亚组，缓冲剂配方和试剂盒的生产方法，以及使用这些干粉状营养培养基，补充剂，亚组和缓冲液制剂培养原核和真核细胞的方法。 提供了生产无菌粉末状介质或补充剂（例如，粉末状FBS，粉状转铁蛋白，粉末状胰岛素，粉末状器官提取物，粉末生长因子），培养基亚组和通过γ辐射的缓冲液制剂的方法。 提供了制备干细胞粉末的方法，包括喷雾干燥细胞悬浮液，以及通过这些方法生产的细胞，培养基，培养基，培养基和缓冲粉末。