公开(公告)号：US20100099191A1

公开(公告)日：2010-04-22

申请号：US12529465

申请日：2007-04-10

申请人： Wolfgang Biekle ,  Peter Hahn

发明人： Wolfgang Biekle ,  Peter Hahn

IPC分类号： C12N15/00 ,  C07H21/00 ,  C12N15/63

CPC分类号： C12N15/111 ,  C12N15/63 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2320/50 ,  C12Q1/6897

摘要： The invention relates to a composition for inhibiting the expression of a target gene in a eukaryotic cell by RNA interference, the composition comprising a genetic construct and the target gene being introduced into the eukaryotic cell by means of said construct, and the construct having a first target gene and a first siRNA tag. The invention also relates to a composition for inhibiting the expression of one or more target genes in a eukaryotic cell by RNA interference. The composition comprises at least two genetic constructs and the target genes are introduced into the eukaryotic cell by means of said constructs, a) the first construct containing a first target gene and a first siRNA tag and b) the second construct containing a second target gene and a second siRNA tag, and the first and the second siRNA tag having different nucleic acid sequences. The invention finally relates to a method for inhibiting the expression of a target gene in a eukaryotic cell. Said method comprises the following steps: a) providing at least one eukaryotic cell, said cell being capable of RNA interference, b) transfecting the cells with a composition according to the invention and c) introducing at least one siRNA that is complementary to a siRNA tag of a transfected construct to inhibit the expression of the target gene that forms a transcription unit together with the siRNA tag.

摘要翻译： 本发明涉及一种用于通过RNA干扰抑制真核细胞中靶基因表达的组合物，所述组合物包含遗传构建体，并且所述靶基因通过所述构建体引入真核细胞，并且所述构建体具有第一 靶基因和第一个siRNA标签。 本发明还涉及通过RNA干扰抑制真核细胞中一种或多种靶基因的表达的组合物。 所述组合物包含至少两个遗传构建体，并且通过所述构建体将靶基因引入真核细胞中，a）含有第一靶基因和第一siRNA标签的第一构建体，和b）含有第二靶基因的第二构建体 和第二siRNA标签，并且第一和第二siRNA标签具有不同的核酸序列。 本发明最终涉及抑制真核细胞中靶基因表达的方法。 所述方法包括以下步骤：a）提供至少一个真核细胞，所述细胞能够进行RNA干扰，b）用根据本发明的组合物转染细胞，和c）引入与siRNA互补的至少一种siRNA 标签，以抑制与siRNA标签一起形成转录单位的靶基因的表达。

公开(公告)号：US08252535B2

公开(公告)日：2012-08-28

申请号：US12529465

申请日：2007-04-10

申请人： Wolfgang Biekle ,  Peter Hahn

发明人： Wolfgang Biekle ,  Peter Hahn

IPC分类号： C12Q1/68 ,  C12N15/11 ,  C12N5/00 ,  C07H21/04

CPC分类号： C12N15/111 ,  C12N15/63 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2320/50 ,  C12Q1/6897

摘要： The invention relates to a method for inhibiting the expression of a target gene in a eukaryotic cell. The method includes the following steps: a) providing at least one eukaryotic cell, the cell being capable of RNA interference, b) transfecting the eukaryotic cell with a composition that includes a genetic construct that includes an siRNA tag, and a target gene that forms a transcription unit together with the siRNA tag, and c) introducing at least one siRNA that is complementary to the siRNA tag of the transfected genetic construct to inhibit the expression of the target gene.

摘要翻译： 本发明涉及一种抑制真核细胞中靶基因表达的方法。 该方法包括以下步骤：a）提供至少一个真核细胞，所述细胞能够进行RNA干扰，b）用包含siRNA标签的遗传构建体和形成的靶基因的组合物转染真核细胞 转录单元和siRNA标签，以及c）引入至少一个与转染的遗传构建体的siRNA标签互补的siRNA，以抑制靶基因的表达。