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    METHOD FOR TRANSFECTING A EUKARYOTIC CELL
    3.
    发明申请
    METHOD FOR TRANSFECTING A EUKARYOTIC CELL 审中-公开
    转染真核细胞的方法

    公开(公告)号：US20130109095A1

    公开(公告)日：2013-05-02

    申请号：US13697188

    申请日：2011-05-06

    申请人： Peter Hahn ,  Anja Grewe

    发明人： Peter Hahn ,  Anja Grewe

    IPC分类号： C12N15/11

    CPC分类号： C12N15/11 ,  C12N15/87 ,  C12N15/88

    摘要： The present invention relates to a method for transfecting a eukaryotic cell, wherein a compound comprising at least one saccharide and at least one protein and optionally a salt and/or a component stabilizing pH is brought into contact with a nucleic acid and a transfection reagent, a complex of nucleic acid and transfection reagent is formed by means of interacting with the composition, and the complex is transfected into a eukaryotic cell.

    摘要翻译： 本发明涉及转染真核细胞的方法，其中包含至少一种糖和至少一种蛋白质和任选的盐和/或稳定pH的成分的化合物与核酸和转染试剂接触， 通过与组合物相互作用形成核酸和转染试剂的复合物，并将复合物转染入真核细胞。

    APPARATUS AND METHOD FOR MANAGING A POWER SOURCE
    4.
    发明申请
    APPARATUS AND METHOD FOR MANAGING A POWER SOURCE 审中-公开
    用于管理电源的装置和方法

    公开(公告)号：US20110109165A1

    公开(公告)日：2011-05-12

    申请号：US12825434

    申请日：2010-06-29

    申请人： Peter Hahn ,  Nils Haustein ,  Rainer Krause

    发明人： Peter Hahn ,  Nils Haustein ,  Rainer Krause

    IPC分类号： H02J4/00

    CPC分类号： H02J3/28 ,  H02J3/383 ,  Y02B10/14 ,  Y02E10/563 ,  Y02E10/566 ,  Y02E70/30 ,  Y10T307/658

    摘要： An electrical power management apparatus and method in which a public power grid, a renewable energy source, an energy storage element and a domestic power supply network are interconnected through a controllable switch which connects the various components together in combinations which are advantageous to sensed power load and consumption circumstances.

    摘要翻译： 一种电力管理装置和方法，其中公共电网，可再生能源，能量存储元件和家用电源网络通过可控开关互连，所述可控开关将组合中的各种组件连接在一起，这对于感测的功率负载是有利的 和消费情况。

    Hard disk drive suspension with reduced PSA change due to humidity and temperature variations
    5.
    发明授权
    Hard disk drive suspension with reduced PSA change due to humidity and temperature variations 有权
    由于湿度和温度变化导致PSA变化减小的硬盘驱动器悬架

    公开(公告)号：US08441761B1

    公开(公告)日：2013-05-14

    申请号：US12983613

    申请日：2011-01-03

    申请人： Peter Hahn ,  Wei Keat Chai ,  Kuen Chee Ee

    发明人： Peter Hahn ,  Wei Keat Chai ,  Kuen Chee Ee

    IPC分类号： G11B5/60 ,  G11B21/21

    CPC分类号： G11B5/4826 ,  G11B5/4833

    摘要： In a disk drive suspension circuit, in order to improve stability of the stable pitch static attitude (PSA) over changes in humidity, the suspended portion of the flexible circuit and/or the gimbal support arm are modified to change their response(s) to humidity. In one embodiment, hygroscopic material such as the material of the insulating layer are added to the stainless steel gimbal support arm, so that in response to humidity it bends in the opposite direction as the suspended portion of the circuit. In another embodiment, additional hygroscopic material is added to the circuit, and/or the polyimide insulating layer or the coverlayer are selectively removed, so that the net bend in the suspended portion of the circuit over humidity is reduced, ideally to a condition of zero or close to zero net bending.

    摘要翻译： 在磁盘驱动器悬架电路中，为了提高稳定的俯仰静止姿态（PSA）与湿度变化的稳定性，柔性电路和/或万向支撑臂的悬挂部分被修改以将其响应改变为 湿度。 在一个实施例中，诸如绝缘层材料的吸湿材料被添加到不锈钢万向架支撑臂中，使得响应于湿度，其沿与电路的悬挂部分相反的方向弯曲。 在另一个实施例中，附加的吸湿材料被添加到电路中，和/或选择性地去除聚酰亚胺绝缘层或覆盖层，使得在湿度下电路悬挂部分的净弯曲减小，理想地为零 或接近零净弯。

    RNA INTERFERENCE TAGS
    7.
    发明申请
    RNA INTERFERENCE TAGS 有权
    RNA干扰标签

    公开(公告)号：US20100099191A1

    公开(公告)日：2010-04-22

    申请号：US12529465

    申请日：2007-04-10

    申请人： Wolfgang Biekle ,  Peter Hahn

    发明人： Wolfgang Biekle ,  Peter Hahn

    IPC分类号： C12N15/00 ,  C07H21/00 ,  C12N15/63

    CPC分类号： C12N15/111 ,  C12N15/63 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2320/50 ,  C12Q1/6897

    摘要： The invention relates to a composition for inhibiting the expression of a target gene in a eukaryotic cell by RNA interference, the composition comprising a genetic construct and the target gene being introduced into the eukaryotic cell by means of said construct, and the construct having a first target gene and a first siRNA tag. The invention also relates to a composition for inhibiting the expression of one or more target genes in a eukaryotic cell by RNA interference. The composition comprises at least two genetic constructs and the target genes are introduced into the eukaryotic cell by means of said constructs, a) the first construct containing a first target gene and a first siRNA tag and b) the second construct containing a second target gene and a second siRNA tag, and the first and the second siRNA tag having different nucleic acid sequences. The invention finally relates to a method for inhibiting the expression of a target gene in a eukaryotic cell. Said method comprises the following steps: a) providing at least one eukaryotic cell, said cell being capable of RNA interference, b) transfecting the cells with a composition according to the invention and c) introducing at least one siRNA that is complementary to a siRNA tag of a transfected construct to inhibit the expression of the target gene that forms a transcription unit together with the siRNA tag.

    摘要翻译： 本发明涉及一种用于通过RNA干扰抑制真核细胞中靶基因表达的组合物，所述组合物包含遗传构建体，并且所述靶基因通过所述构建体引入真核细胞，并且所述构建体具有第一 靶基因和第一个siRNA标签。 本发明还涉及通过RNA干扰抑制真核细胞中一种或多种靶基因的表达的组合物。 所述组合物包含至少两个遗传构建体，并且通过所述构建体将靶基因引入真核细胞中，a）含有第一靶基因和第一siRNA标签的第一构建体，和b）含有第二靶基因的第二构建体 和第二siRNA标签，并且第一和第二siRNA标签具有不同的核酸序列。 本发明最终涉及抑制真核细胞中靶基因表达的方法。 所述方法包括以下步骤：a）提供至少一个真核细胞，所述细胞能够进行RNA干扰，b）用根据本发明的组合物转染细胞，和c）引入与siRNA互补的至少一种siRNA 标签，以抑制与siRNA标签一起形成转录单位的靶基因的表达。

    Load beam having concave stiffener with locating flanges
    8.
    发明授权
    Load beam having concave stiffener with locating flanges 有权
    负载梁具有带有定位法兰的凹形加强件

    公开(公告)号：US07551404B1

    公开(公告)日：2009-06-23

    申请号：US11233191

    申请日：2005-09-21

    申请人： Johnathan Phu ,  Chu Wong ,  Peter Hahn

    发明人： Johnathan Phu ,  Chu Wong ,  Peter Hahn

    IPC分类号： G11B5/55 ,  G11B21/08

    CPC分类号： G11B5/4833

    摘要： A disk drive suspension has a mount plate, an outwardly tapered load beam supported by the mount plate and having a base portion, a hinge portion, a beam portion having a first face with turned edges and a flexure supported by the beam portion distally of said beam portion face, and a generally congruent tapered stiffener having a central portion of a given height that is concave and open to the beam portion. The stiffener has left and right flange portions extending laterally from the central portion to the beam portion upturned edges to locate said central portion centrally on the beam portion face.

    摘要翻译： 磁盘驱动器悬架具有安装板，由安装板支撑的向外锥形的负载梁，其具有基部，铰链部分，具有带有转动边缘的第一面的梁部分和由所述梁部分的远端支撑的挠曲件 梁部分面和大致一致的锥形加强件，其具有给定高度的中心部分，该中心部分是凹形的并且对梁部分敞开。 加强件具有从中心部分横向延伸到梁部分上翻边缘的左右凸缘部分，以将所述中心部分中心地定位在梁部分面上。

    Mounting assembly for a disk drive
    9.
    发明授权
    Mounting assembly for a disk drive 失效
    磁盘驱动器的安装组件

    公开(公告)号：US06297928B1

    公开(公告)日：2001-10-02

    申请号：US09203775

    申请日：1998-12-02

    申请人： Peter Hahn ,  Arthur Lin ,  Shin John Choi ,  Rodney Ngai

    发明人： Peter Hahn ,  Arthur Lin ,  Shin John Choi ,  Rodney Ngai

    IPC分类号： G11B3314

    CPC分类号： G11B33/121 ,  G11B33/08

    摘要： A mounting assembly for securing a disk drive to a frame of a computer is provided herein. The mounting assembly includes three rigid mounts and a single flexible mount. Each rigid mount rigidly secures the drive housing to the frame and prevents degradation of performance of the disk drive. The flexible mount diminishes the level of vibration transferred from the frame to drive housing. Further, the flexible mount facilitates flexing of the drive housing intermediate the flexible mount and the rigid mounts. This reduces the effects of a shock pulse to the disk drive and inhibits head slap between a transducer head and a storage disk.

    摘要翻译： 本文提供了用于将盘驱动器固定到计算机的框架的安装组件。 安装组件包括三个刚性支架和一个单一的柔性支架。 每个刚性安装座将驱动器壳体刚性地固定在框架上，并防止磁盘驱动器的性能下降。 柔性安装座减小了从框架传动到驱动外壳的振动水平。 此外，柔性安装件有助于柔性支架和刚性支架之间的驱动壳体的弯曲。 这减少了冲击脉冲对磁盘驱动器的影响，并且抑制了传感器头和存储盘之间的头部拍打。

    Method for the automatic gutting of fish and an apparatus for implementing the method
    10.
    发明授权
    Method for the automatic gutting of fish and an apparatus for implementing the method 失效
    鱼类自动排水方法及其实施方法

    公开(公告)号：US5735735A

    公开(公告)日：1998-04-07

    申请号：US551340

    申请日：1995-11-01

    申请人： Peter Hahn ,  Gunther Pinkerneil ,  Klaus Dieter Nuske

    发明人： Peter Hahn ,  Gunther Pinkerneil ,  Klaus Dieter Nuske

    IPC分类号： A22C25/08 ,  A22C25/14

    CPC分类号： A22C25/145

    摘要： In a method for automatically gutting fish of the catfish species, the belly cavity is opened around the anal orifice either before or after opening the gills by severing the gullet and opening the blood stream, and the contents of the belly cavity are removed by pushing these from one of the openings of the cavity out of the other. In a machine for carrying out this method, the fish are conveyed sideways by a recessed conveyor. The belly cavity is opened in the vicinity of the anus by separating away the cartilaginous plate carrying the ventral fins. The contents of the ventral cavity are removed by a rotary body rotating synchronously with the conveyor and having tools successively arranged in a cantilevered helix around its circumference which comprise a clearing tool with scraping segments, a brush tool, a high-pressure spray device and a cutting tool for slitting open the belly cavity along its length.

    摘要翻译： 在用于自动cat鱼鲶鱼的方法中，通过切断食道并打开血流，在打开鳃之前或之后，在肛门孔周围打开腹腔，并通过推动这些腹腔来除去腹腔内容物 从其中一个孔的一个开口。 在用于执行该方法的机器中，鱼被凹进的输送机侧向传送。 通过将携带腹鳍的软骨板分开，将腹腔打开在肛门附近。 通过与输送机同步旋转的旋转体除去腹腔的内容物，并且具有围绕其圆周依次布置在悬臂螺旋中的工具，其包括具有刮削段的清除工具，刷子工具，高压喷雾装置和 切割工具用于沿着其长度切开腹部腔。