摘要:
The present invention relates to a gene being capable of modifying resistance against a heavy metal or salt, or accumulation properties, a recombination vector including the genes, and a transformant using the recombination vector. A gene having heavy metal resistance and accumulation properties includes a sequence encoding a transmembrane protein having five times repeated similar four transmembrane domains. A recombination vector includes the gene having heavy metal resistance and accumulation properties, and further includes a salt or drought resistance gene having at least one selected from the group consisting of a sequence encoding an ABC transporter including twice repeated six transmembrane domains and ATP-binding domains.
摘要:
The invention relates to a transgenic plant or yeast comprising a DNA molecule encoding fungal ATP-binding cassette (ABC) transporter protein, which confers resistance to, and/or accumulation of heavy metals and herbicides. The invention also relates to methods of producing transgenic plants expressing fungal YHL035C protein, which can be used for removing heavy metals and herbicides from polluted soil or water.
摘要:
The present invention relates to an isolated DNA encoding fungal MRP (multidrug resistance associated protein) subfamily of ATP-binding cassette (ABC) transporter protein which confers organisms with resistance to and/or accumulation of toxic materials such as heavy metals and herbicides, vectors containing the isolated DNA, and organisms transformed with the isolated DNA. Organisms transformed with the fungal MRP subfamily of ABC transporters of the present invention can be used for remediation of environment polluted with toxic materials. For example, the transformed plants can be used for cleaning polluted soil or water, and thereby providing an environmentally-friendly way to remediate polluted resources with low cost.
摘要:
The present invention relates to a gene being capable of modifying resistance against a heavy metal or salt, or accumulation properties, a recombination vector including the genes, and a transformant using the recombination vector. A gene having heavy metal resistance and accumulation properties includes a sequence encoding a transmembrane protein having five times repeated similar four transmembrane domains. A recombination vector includes the gene having heavy metal resistance and accumulation properties, and further includes a salt or drought resistance gene having at least one selected from the group consisting of a sequence encoding an ABC transporter including twice repeated six transmembrane domains and ATP-binding domains; a sequence encoding a protein including a GTP-binding domain and a CaaL domain (geranylgeranylation motif) being capable of transferring its position from a cytoplasm to cell membrane
摘要:
The present invention relates to novel Pcr family genes that confer tolerance to heavy metals to plants and microorganisms. More particularly, the present invention relates to novel Pcr family genes that confer tolerance to cadmium. The invention also relates to plant and microorganisms transformed with any of the Pcr genes thereby having improved resistance to and increased level of toxic materials, and methods of removing heavy metals from contaminated soil and water contaminated by growing the Pcr-transformed plants on the contaminated soils and water.
摘要:
A composition for transportation of phytochelatin including two DNA molecule encoding two members of ABCC (multidrug resistance-associated protein (MRP)) subfamily of ATP-binding cassette (ABC) transporter protein in a plant is provided. The composition for transportation of phytochelatin can be useful in accumulating and sequestering phytochelatin alone or in combination with a harmful substance by transporting the phytochelatin and/or harmful substance into the vacuole of a cell, thereby reducing a content of the toxic substance from cytosol, and also reducing it from translocation from the root to the shoot of a plant. Therefore, the DNA molecule can be useful to develop a crop in which the toxic substance present in edible parts, such as leaves, stems and fruits, of the plant is reduced in content. Furthermore, the DNA molecule can be useful to develop inedible plants for phytoremediation or phytoextraction due to an increase in accumulated amount of and resistance against arsenic, an arsenic compound and cadmium, thereby making it possible to achieve phytoremediation or phytoextraction in an environmentally friendly and economic manner.
摘要:
The present invention relates to novel Pcr family genes which confer tolerance to heavy metals to organisms. More particularly, the present invention relates to novel Pcr family genes which confer tolerance to cadmium. Organisms transformed with any of the Pcr genes thereby have improved resistance to and increased level of toxic materials. Soil and water contaminated with heavy metals can be cleaned in an environment-friendly and economical manner by growing the Pcr-transformed organism, especially a plant transformed with the Pcr gene.