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    Detection method of nucleic acid and kit and using thereof
    1.
    发明授权
    Detection method of nucleic acid and kit and using thereof 有权
    核酸和试剂盒的检测方法及其应用

    公开(公告)号：US09404153B2

    公开(公告)日：2016-08-02

    申请号：US14007201

    申请日：2011-04-12

    申请人： Xingjie Liang ,  Hua Deng ,  Xiaowei Ma ,  Chan Li

    发明人： Xingjie Liang ,  Hua Deng ,  Xiaowei Ma ,  Chan Li

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q1/6816 ,  C12Q1/689 ,  C12Q2565/107

    摘要： A detection method of nucleic acid is provided. The method includes: providing nucleic acid to be tested, making the nucleic acid to be tested react under asymmetric PCR conditions with a pair of primers for target nucleic acid amplification, DNA polymerase, adenine deoxyribonucleotide, guanine deoxyribonucleotide, cytosine deoxyribonucleotide and thymidine deoxyribonucleotide in a PCR buffer solution, mixing the reaction product and liquid that contains probe molecules, and judging whether the nucleic acid to be tested contains the target nucleic acid by observing the obtained mixture color or color change. A kit is also provided that can be used in the nucleic acid detection by the said method. Application of the method and the kit in inspection and quarantine is also provided. The method is a quick and easy, sensitive and a specific detection method of nucleic acid with direct observation using naked eyes. The method does not need additional equipment.

    摘要翻译： 提供核酸的检测方法。 该方法包括：提供待测试的核酸，使待测试的核酸在不对称PCR条件下与靶核酸扩增的一对引物，DNA聚合酶，腺嘌呤脱氧核糖核苷酸，鸟嘌呤脱氧核糖核苷酸，胞嘧啶脱氧核糖核苷酸和胸苷脱氧核糖核苷酸反应 PCR缓冲溶液，混合含有探针分子的反应产物和液体，并通过观察获得的混合物颜色或颜色变化来判断待测核酸是否含有靶核酸。 还提供了可用于所述方法的核酸检测中的试剂盒。 还提供了该方法和试剂盒在检验检疫中的应用。 该方法是使用肉眼直观观察的核酸的快速，容易，灵敏和具体的检测方法。 该方法不需要额外的设备。

    DETECTION METHOD OF NUCLEIC ACID AND KIT AND USING THEREOF
    3.
    发明申请
    DETECTION METHOD OF NUCLEIC ACID AND KIT AND USING THEREOF 有权
    核酸和试剂盒的检测方法及其使用

    公开(公告)号：US20140017671A1

    公开(公告)日：2014-01-16

    申请号：US14007201

    申请日：2011-04-12

    申请人： Xingjie Liang ,  Hua Deng ,  Xiaowei Ma

    发明人： Xingjie Liang ,  Hua Deng ,  Xiaowei Ma

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q1/6816 ,  C12Q1/689 ,  C12Q2565/107

    摘要： A detection method of nucleic acid is provided. The method includes: providing nucleic acid to be tested, making the nucleic acid to be tested react under asymmetric PCR conditions with a pair of primers for target nucleic acid amplification, DNA polymerase, adenine deoxyribonucleotide, guanine deoxyribonucleotide, cytosine deoxyribonucleotide and thymidine deoxyribonucleotide in a PCR buffer solution, mixing the reaction product and liquid that contains probe molecules, and judging whether the nucleic acid to be tested contains the target nucleic acid by observing the obtained mixture color or color change. A kit is also provided that can be used in the nucleic acid detection by the said method. Application of the method and the kit in inspection and quarantine is also provided. The method is a quick and easy, sensitive and a specific detection method of nucleic acid with direct observation using naked eyes. The method does not need additional equipment.

    摘要翻译： 提供核酸的检测方法。 该方法包括：提供待测试的核酸，使待测试的核酸在不对称PCR条件下与靶核酸扩增的一对引物，DNA聚合酶，腺嘌呤脱氧核糖核苷酸，鸟嘌呤脱氧核糖核苷酸，胞嘧啶脱氧核糖核苷酸和胸苷脱氧核糖核苷酸反应 PCR缓冲溶液，混合含有探针分子的反应产物和液体，并通过观察获得的混合物颜色或颜色变化来判断待测核酸是否含有靶核酸。 还提供了可用于所述方法的核酸检测中的试剂盒。 还提供了该方法和试剂盒在检验检疫中的应用。 该方法是使用肉眼直观观察的核酸的快速，容易，灵敏和具体的检测方法。 该方法不需要额外的设备。