公开(公告)号：US5118615A

公开(公告)日：1992-06-02

申请号：US023818

申请日：1987-03-09

申请人： Hisayuki Matsuo ,  Kenji Kangawa ,  Yujiro Hayashi ,  Shinzo Oikawa ,  Takehiro Oshima ,  Shoji Tanaka ,  Hiroshi Nakazato ,  Yasunori Tawaragi

发明人： Hisayuki Matsuo ,  Kenji Kangawa ,  Yujiro Hayashi ,  Shinzo Oikawa ,  Takehiro Oshima ,  Shoji Tanaka ,  Hiroshi Nakazato ,  Yasunori Tawaragi

IPC分类号： A61K38/00 ,  C07K14/58 ,  C12N15/12 ,  C12N15/70

CPC分类号： C07K14/58 ,  C12N15/70 ,  A61K38/00 ,  Y10S514/869

摘要： Disclosed is a DNA fragment comprising a base sequence coding for a peptide occurring in human atrium cordis and having diuretic action or a precursor of the peptide, plasmids containing the DNA fragment, microorganisms transformed with the plasmid, and a process for production of the peptide using the transformant.Also disclosed is a new peptide consisting of 126 amino acids occurring in human atrium cordis and a precursor thereof. The peptide has notable diuretic action and hypotensive or antihypertensive actions.

摘要翻译： 公开了一种DNA片段，其包含编码人心房中出现的具有利尿作用或肽前体的肽的碱基序列，含有该DNA片段的质粒，用质粒转化的微生物，以及使用 转化子。 还公开了由人心房中出现的126个氨基酸及其前体组成的新肽。 肽具有显着的利尿作用和降血压或降压作用。

公开(公告)号：US6034231A

公开(公告)日：2000-03-07

申请号：US765830

申请日：1991-09-26

申请人： Shoji Tanaka ,  Kayoko Fuchimura ,  Yasunori Tawaragi ,  Hisayuki Matsuo ,  Kenji Kanagawa ,  Naoto Minamino

发明人： Shoji Tanaka ,  Kayoko Fuchimura ,  Yasunori Tawaragi ,  Hisayuki Matsuo ,  Kenji Kanagawa ,  Naoto Minamino

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/22 ,  A61P7/10 ,  C07K14/00 ,  C07K14/575 ,  C07K14/58 ,  C12N15/16 ,  C07H17/00

CPC分类号： C07K14/58 ,  A61K38/00

摘要： The gene of a human CNP (human C-type natriuretic peptide), as well as the hCNP precursor protein that is encoded by said gene. The hCNP precursor is represented by the following amino acid sequence.Met His Leu Ser Gln Leu Leu Ala Cys Ala - Leu Leu Leu Thr Leu Leu Ser Leu Arg Pro - Ser Glu Ala Lys Pro Gly Ala Pro Pro Lys - Val Pro Arg Thr Pro Pro Ala Glu Glu Leu - Ala Glu Pro Gln Ala Ala Gly Gly Gly Gln - Lys Lys Gly Asp Lys Ala Pro Gly Gly Gly - Gly Ala Asn Leu Lys Gly Asp Arg Ser Arg - Leu Leu Arg Asp Leu Arg Val Asp Thr Lys - Ser Arg Ala Ala Trp Ala Arg Leu Leu Gln - Glu His Pro Asn Ala Arg Lys Tyr Lys Gly - Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys - Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser - Met Ser Gly Leu Gly Cys. The hCNP precursor and its derivatives are novel and have natriuretic and hypotensive activities.

摘要翻译： 人CNP（人C型利钠肽）的基因以及由所述基因编码的hCNP前体蛋白。 hCNP前体由以下氨基酸序列表示。 - Met His Leu Ser Gln Leu Leu Ala Cys Ala - - Leu Leu Leu Leu Leu Leu Leu Ser Leu Arg Pro - - Ser Glu Ala Lys Pro Gly Ala Pro Pro Lys - - Val Pro Arg Thr Pro Pro Ala Glu Glu Leu - - Ala Glu Pro Gln Ala Ala Gly Gly Gly Gln - - Lys Lys Gly Asp Lys Ala Pro Gly Gly Gly - - Gly Ala Asn Leu Lys Gly Asp Arg Ser Arg - - Leu Leu Arg Asp Leu Arg Val Asp Thr Lys - - Ser Arg Ala Ala Trp Ala Arg Leu Leu Gln - - Glu His Pro Asn Ala Arg Lys Tyr Lys Gly - - Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys - Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser - - Met Ser Gly Leu Gly Cys。 - hCNP前体及其衍生物是新颖的，具有钠尿和降血压活性。

公开(公告)号：US6020168A

公开(公告)日：2000-02-01

申请号：US728220

申请日：1991-07-12

申请人： Hisayuki Matsuo ,  Kenji Kangawa ,  Naoto Minamino ,  Shoji Tanaka ,  Kayoko Fuchimura ,  Yasunori Tawaragi

发明人： Hisayuki Matsuo ,  Kenji Kangawa ,  Naoto Minamino ,  Shoji Tanaka ,  Kayoko Fuchimura ,  Yasunori Tawaragi

IPC分类号： C12N15/09 ,  A61K38/00 ,  C07H21/04 ,  C07K14/575 ,  C07K14/58 ,  C12N15/16 ,  C12P21/02 ,  C12R1/19 ,  C12P21/06 ,  C07H17/00 ,  C07K14/00

CPC分类号： C07K14/58 ,  A61K38/00

摘要： The gene and cDNA of a procine derived CNP (C-type natriuretic peptide), and a procine derived CNP precursor protein and derivatives thereof are disclosed. The proceine derivative CNP precursor is represented by the following amino acid sequence:Met His Leu Ser Gln Leu Leu Ala Cys Ala - Leu Leu Leu Thr Leu Leu Ser Leu Arg Pro - Ser Glu Ala Lys Pro Gly Ala Pro Pro Lys - Val Pro Arg Thr Pro Pro Gly Glu Glu Val - Ala Glu Pro Gln Ala Ala Gly Gly Gly Gln - Lys Lys Gly Asp Lys Thr Pro Gly Gly Gly - Gly Ala Asn Leu Lys Gly Asp Arg Ser Arg - Leu Leu Arg Asp Leu Arg Val Asp Thr Lys - Ser Arg Ala Ala Trp Ala Arg Leu Leu His - Glu His Pro Asn Ala Arg Lys Tyr Lys Gly - Gly Asn Lys Lys Gly Leu Ser Lys Gly Cys - Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser These derivatives are novel and have natriuretic and hypotensive activities.

摘要翻译： 公开了源自CNP（C型利尿钠肽）的基因和cDNA，以及源自CNP前体蛋白及其衍生物的衍生物。 程序衍生物CNP前体由以下氨基酸序列表示： - Met His Leu Ser Gln Leu Leu Ala Cys Ala - - Leu Leu Leu Thr Leu Leu Ser Leu Arg Pro - Ser Glu Ala Lys Pro Gly Ala Pro Pro Lys - - Val Pro Arg Thr Pro Pro Gly Glu Glu Val - - Ala Glu Pro Gln Ala Ala Gly Gly Gly Gln - - Lys Lys Gly Asp Lys Thr Pro Gly Gly Gly - - Gly Ala Asn Leu Lys Gly Asp Arg Ser Arg - - Leu Leu Arg Asp Leu Arg Val Asp Thr Lys - - Ser Arg Ala Ala Trp Ala Arg Leu Leu His - - Glu His Pro Asn Ala Arg Lys Tyr Lys Gly - - Gly Asn Lys Lys Gly Leu Ser Lys Gly Cys - Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser - - Met Ser Gly Leu Gly Cys。 - 这些衍生物是新颖的，具有钠尿和降血压活性。