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    Porcine CNP gene and precursor protein
    2.
    发明授权
    Porcine CNP gene and precursor protein 失效
    猪CNP基因和前体蛋白

    公开(公告)号：US6020168A

    公开(公告)日：2000-02-01

    申请号：US728220

    申请日：1991-07-12

    申请人： Hisayuki Matsuo ,  Kenji Kangawa ,  Naoto Minamino ,  Shoji Tanaka ,  Kayoko Fuchimura ,  Yasunori Tawaragi

    发明人： Hisayuki Matsuo ,  Kenji Kangawa ,  Naoto Minamino ,  Shoji Tanaka ,  Kayoko Fuchimura ,  Yasunori Tawaragi

    IPC分类号： C12N15/09 ,  A61K38/00 ,  C07H21/04 ,  C07K14/575 ,  C07K14/58 ,  C12N15/16 ,  C12P21/02 ,  C12R1/19 ,  C12P21/06 ,  C07H17/00 ,  C07K14/00

    CPC分类号： C07K14/58 ,  A61K38/00

    摘要： The gene and cDNA of a procine derived CNP (C-type natriuretic peptide), and a procine derived CNP precursor protein and derivatives thereof are disclosed. The proceine derivative CNP precursor is represented by the following amino acid sequence:Met His Leu Ser Gln Leu Leu Ala Cys Ala - Leu Leu Leu Thr Leu Leu Ser Leu Arg Pro - Ser Glu Ala Lys Pro Gly Ala Pro Pro Lys - Val Pro Arg Thr Pro Pro Gly Glu Glu Val - Ala Glu Pro Gln Ala Ala Gly Gly Gly Gln - Lys Lys Gly Asp Lys Thr Pro Gly Gly Gly - Gly Ala Asn Leu Lys Gly Asp Arg Ser Arg - Leu Leu Arg Asp Leu Arg Val Asp Thr Lys - Ser Arg Ala Ala Trp Ala Arg Leu Leu His - Glu His Pro Asn Ala Arg Lys Tyr Lys Gly - Gly Asn Lys Lys Gly Leu Ser Lys Gly Cys - Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser These derivatives are novel and have natriuretic and hypotensive activities.

    摘要翻译： 公开了源自CNP（C型利尿钠肽）的基因和cDNA，以及源自CNP前体蛋白及其衍生物的衍生物。 程序衍生物CNP前体由以下氨基酸序列表示： - Met His Leu Ser Gln Leu Leu Ala Cys Ala - - Leu Leu Leu Thr Leu Leu Ser Leu Arg Pro - Ser Glu Ala Lys Pro Gly Ala Pro Pro Lys - - Val Pro Arg Thr Pro Pro Gly Glu Glu Val - - Ala Glu Pro Gln Ala Ala Gly Gly Gly Gln - - Lys Lys Gly Asp Lys Thr Pro Gly Gly Gly - - Gly Ala Asn Leu Lys Gly Asp Arg Ser Arg - - Leu Leu Arg Asp Leu Arg Val Asp Thr Lys - - Ser Arg Ala Ala Trp Ala Arg Leu Leu His - - Glu His Pro Asn Ala Arg Lys Tyr Lys Gly - - Gly Asn Lys Lys Gly Leu Ser Lys Gly Cys - Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser - - Met Ser Gly Leu Gly Cys。 - 这些衍生物是新颖的，具有钠尿和降血压活性。

    Human CNP gene and precursor protein
    3.
    发明授权
    Human CNP gene and precursor protein 失效
    人CNP基因和前体蛋白

    公开(公告)号：US6034231A

    公开(公告)日：2000-03-07

    申请号：US765830

    申请日：1991-09-26

    申请人： Shoji Tanaka ,  Kayoko Fuchimura ,  Yasunori Tawaragi ,  Hisayuki Matsuo ,  Kenji Kanagawa ,  Naoto Minamino

    发明人： Shoji Tanaka ,  Kayoko Fuchimura ,  Yasunori Tawaragi ,  Hisayuki Matsuo ,  Kenji Kanagawa ,  Naoto Minamino

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/22 ,  A61P7/10 ,  C07K14/00 ,  C07K14/575 ,  C07K14/58 ,  C12N15/16 ,  C07H17/00

    CPC分类号： C07K14/58 ,  A61K38/00

    摘要： The gene of a human CNP (human C-type natriuretic peptide), as well as the hCNP precursor protein that is encoded by said gene. The hCNP precursor is represented by the following amino acid sequence.Met His Leu Ser Gln Leu Leu Ala Cys Ala - Leu Leu Leu Thr Leu Leu Ser Leu Arg Pro - Ser Glu Ala Lys Pro Gly Ala Pro Pro Lys - Val Pro Arg Thr Pro Pro Ala Glu Glu Leu - Ala Glu Pro Gln Ala Ala Gly Gly Gly Gln - Lys Lys Gly Asp Lys Ala Pro Gly Gly Gly - Gly Ala Asn Leu Lys Gly Asp Arg Ser Arg - Leu Leu Arg Asp Leu Arg Val Asp Thr Lys - Ser Arg Ala Ala Trp Ala Arg Leu Leu Gln - Glu His Pro Asn Ala Arg Lys Tyr Lys Gly - Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys - Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser - Met Ser Gly Leu Gly Cys. The hCNP precursor and its derivatives are novel and have natriuretic and hypotensive activities.

    摘要翻译： 人CNP（人C型利钠肽）的基因以及由所述基因编码的hCNP前体蛋白。 hCNP前体由以下氨基酸序列表示。 - Met His Leu Ser Gln Leu Leu Ala Cys Ala - - Leu Leu Leu Leu Leu Leu Leu Ser Leu Arg Pro - - Ser Glu Ala Lys Pro Gly Ala Pro Pro Lys - - Val Pro Arg Thr Pro Pro Ala Glu Glu Leu - - Ala Glu Pro Gln Ala Ala Gly Gly Gly Gln - - Lys Lys Gly Asp Lys Ala Pro Gly Gly Gly - - Gly Ala Asn Leu Lys Gly Asp Arg Ser Arg - - Leu Leu Arg Asp Leu Arg Val Asp Thr Lys - - Ser Arg Ala Ala Trp Ala Arg Leu Leu Gln - - Glu His Pro Asn Ala Arg Lys Tyr Lys Gly - - Ala Asn Lys Lys Gly Leu Ser Lys Gly Cys - Phe Gly Leu Lys Leu Asp Arg Ile Gly Ser - - Met Ser Gly Leu Gly Cys。 - hCNP前体及其衍生物是新颖的，具有钠尿和降血压活性。

    Method for screening compounds regulating the expression of human-inducible nitric oxide synthase
    4.
    发明授权
    Method for screening compounds regulating the expression of human-inducible nitric oxide synthase 失效
    筛选调节人诱导型一氧化氮合酶表达的化合物的方法

    公开(公告)号：US06203982B1

    公开(公告)日：2001-03-20

    申请号：US09068880

    申请日：1998-09-02

    申请人： Youichi Nunokawa ,  Shinzo Oikawa ,  Shoji Tanaka

    发明人： Youichi Nunokawa ,  Shinzo Oikawa ,  Shoji Tanaka

    IPC分类号： A61K3821

    CPC分类号： C07D295/185 ,  A61K31/12 ,  A61K31/165 ,  A61K31/215 ,  C07C50/06 ,  C07C50/30 ,  C12N9/0075 ,  C12Q1/6897 ,  G01N2500/04

    摘要： A plasmid was constructed, in which the hiNOS structural gene was replaced with the luciferase structural gene as a reporter gene, with retaining functions of the hiNOS gene 5′-promoter region and 3′-untranslated region. This plasmid was stably transfected into human cell lines. The above transformed cells selectively expressed the reporter gene in the presence of inducers. It has become possible, by examining the reporter gene expression in these transformed cells, to simply and easily screen, with high sensitivity, a compound which is expected to be useful for treating inflammations and sepsis by suppressing the hiNOS expression, or a compound which is expected to be useful for antitumor, antiviral, and vascular restenosis prevention treatments by the hiNOS induction.

    摘要翻译： 构建了质粒，其中HiNOS结构基因被作为报告基因的荧光素酶结构基因替代，具有hiNOS基因5'-启动子区和3'非翻译区的保留功能。 将该质粒稳定转染入人细胞系。 上述转化细胞在诱导物存在下选择性地表达报道基因。 通过检查这些转化细胞中的报告基因表达，可以以高灵敏度简单且容易地筛选预期可用于通过抑制hiNOS表达来治疗炎症和败血症的化合物，或者是化合物 预期通过hiNOS诱导对抗肿瘤，抗病毒和血管再狭窄的预防治疗有用。

    CNP analog peptides and their use
    8.
    发明授权
    CNP analog peptides and their use 失效
    CNP类似物及其用途

    公开(公告)号：US5434133A

    公开(公告)日：1995-07-18

    申请号：US828450

    申请日：1992-01-31

    申请人： Shoji Tanaka ,  Yoshiharu Minamitake ,  Yasuo Kitajima ,  Mayumi Furuya ,  Hisayuki Matsuo

    发明人： Shoji Tanaka ,  Yoshiharu Minamitake ,  Yasuo Kitajima ,  Mayumi Furuya ,  Hisayuki Matsuo

    IPC分类号： A61K38/22 ,  A61K38/00 ,  A61P43/00 ,  C07K7/08 ,  C07K14/575 ,  C07K14/58 ,  C12N15/16 ,  C07K14/00 ,  A61K38/16 ,  A61K38/17 ,  C07K14/435

    CPC分类号： C07K14/58 ,  A61K38/00 ,  Y02P20/55

    摘要： Novel peptides represented by the general formula: ##STR1## and physiologically acceptable acid addition salts thereof; where (A) represents H-, H-Gly, H-Lys-Gly, H-Ser-Lys-Gly, H-Leu-Ser-Lys-Gly, H-Gly-Leu-Ser-Lys-Gly, H-ser, H-Ser-Ser, H-Arg-Ser-Ser, H-Arg-Arg-Ser-Ser, H-Leu-Arg-Arg-Ser-Ser, H-Ser-Leu-Arg-Arg-Ser-Ser;(B) represents H-Cys or Pmp;(C) represents Phe-, pCl-Phe, pF-Phe, pNO.sub.2 -Phe or Cha;(D) represents Ile, Val, Aib, tLeu, Gly or Leu;(E) represents Lys or Arg;(F) represents Ile, Leu or Met;(G) represents Ser or Ala;(H) represents Met or Gln;(I) represents --OH, -Asn-OH, -Asn-Ser-OH, -Asn-Ser-Phe-OH, -Asn-Ser-Phe-Arg-OH or -Asn-Ser-Phe-Arg-Tyr-OH; and the symbol " . . . " represents a disulfide bond;provided that 1) .alpha.-hANP, 2) .alpha.-hANP (7-28) and 3) CNP-22 are excluded from the scope of that general formula.Also disclosed are agents for suppressing the growth of vascular smooth muscle cells that contains those peptides as effective ingredients.

    摘要翻译： 由通式表示的新肽：其加成盐; 其中（A）表示H，H-Gly，H-Lys-Gly，H-Ser-Lys-Gly，H-Leu-Ser-Lys-Gly，H-Gly-Leu-Ser-Lys-Gly， Ser，H-Ser-Ser，H-Arg-Ser-Ser，H-Arg-Arg-Ser-Ser，H-Leu-Arg-Arg-Ser-Ser，H-Ser-Leu-Arg-Arg- 塞尔 （B）代表H-Cys或Pmp; （C）表示Phe-，pCl-Phe，pF-Phe，pNO2-Phe或Cha; （D）代表Ile，Val，Aib，tLeu，Gly或Leu; （E）表示Lys或Arg; （F）表示Ile，Leu或Met; （G）表示Ser或Ala; （H）表示Met或Gln; （I）表示-OH，-Asn-OH，-Asn-Ser-OH，-Asn-Ser-Phe-OH，-Asn-Ser-Phe-Arg-OH或-Asn-Ser-Phe-Arg-Tyr- 哦; 符号“......”代表二硫键; 条件是1）α-hANP，2）α-hANP（7-28）和3）CNP-22被排除在该通式的范围之外。 还公开了抑制含有那些肽作为有效成分的血管平滑肌细胞生长的药剂。

    Expression vector for human TNF
    10.
    发明授权
    Expression vector for human TNF 失效
    人TNF的表达载体

    公开(公告)号：US5059530A

    公开(公告)日：1991-10-22

    申请号：US402675

    申请日：1989-09-05

    申请人： Takehiro Oshima ,  Shoji Tanaka ,  Shigekazu Matsukura

    发明人： Takehiro Oshima ,  Shoji Tanaka ,  Shigekazu Matsukura

    IPC分类号： A61K38/00 ,  C07K14/525 ,  C12N1/21 ,  C12N15/70

    CPC分类号： C07K14/525 ,  C12N15/70 ,  A61K38/00

    摘要： The present invention provides a vector plasmid capable of efficient tumor necrosis factor (TNF) production, a process capable of efficient TNF production in a host transformed with said plasmid and a composition containing the TNF produced by said process.The novel plasmid of the present invention is characterized by having inserted therein a DNA fragment that has a phage-derived promoter region upstream of a structural gene for TNF and in which a DNA fragment containing an E. coli gene-derived transcription termination coding base sequence (terminator) is joined immediately downstream of a base sequence coding for the termination of translation of said structural gene.

    摘要翻译： 本发明提供能够有效的肿瘤坏死因子（TNF）产生的载体质粒，能够在用所述质粒转化的宿主中有效地产生TNF的方法和含有由所述方法产生的TNF的组合物。 本发明的新型质粒的特征在于，在其中插入有在TNF结构基因上游具有噬菌体衍生的启动子区域的DNA片段，其中含有大肠杆菌基因的转录终止编码碱基序列的DNA片段 （终止子）紧接在编码所述结构基因翻译终止的碱基序列的下游。