公开(公告)号：US06242208B1

公开(公告)日：2001-06-05

申请号：US08235238

申请日：1994-05-02

申请人： Yasushi Shirahase ,  Kenji Isshiki ,  Yoshifumi Watazu

发明人： Yasushi Shirahase ,  Kenji Isshiki ,  Yoshifumi Watazu

IPC分类号： C12Q132

CPC分类号： C12Q1/32

摘要： By a process comprising inhibiting LDH2, LDH3, LDH4 and LDH5 in a sample with a protease in the presence of a protein-denaturating agent and then determining LDH1 remaining uninhibited, LDH1 in the sample is easily determined.

摘要翻译： 通过包括在蛋白质变性剂存在下用蛋白酶抑制LDH2，LDH3，LDH4和LDH5，然后测定LDH1保持不受抑制的方法，容易确定样品中的LDH1。

公开(公告)号：US6114134A

公开(公告)日：2000-09-05

申请号：US453474

申请日：1999-12-02

申请人： Koji Kishi ,  Tsutomu Kakuyama ,  Yasushi Shirahase ,  Yoshifumi Watazu

发明人： Koji Kishi ,  Tsutomu Kakuyama ,  Yasushi Shirahase ,  Yoshifumi Watazu

IPC分类号： C12Q1/60 ,  G01N33/92 ,  C12Q1/00

CPC分类号： G01N33/92 ,  C12Q1/60 ,  Y10S435/975

摘要： A method for assaying biological specimens for substances contained in the components of each specimen by the use of one or more kinds of calixarenes; and a reagent comprising one or more kinds of calixarenes. The method utilizes complexes formed by calixarenes and the components of biological specimens, and makes it possible to assay biological specimens for substances contained in the components of each specimen, for example, for cholesterol contained in high-density lipoprotein (HDL), without preliminary separation from the other components of the biological specimen. The method can be conducted by easy and simple operations and lessen measurement errors or problems caused by man, and permits continuous measurement with general-purpose automatic analyzing apparatuses and multi-channel measurement together with other test items.

摘要翻译： 一种通过使用一种或多种杯芳烃来测定每个样品组分中所含物质的生物样品的方法; 和包含一种或多种杯芳烃的试剂。 该方法利用由杯芳烃形成的复合物和生物标本的成分，并且可以测定生物标本中各物质组分中所含的物质，例如高密度脂蛋白（HDL）中所含的胆固醇，而无需初步分离 从生物样本的其他成分。 该方法可以通过简单和简单的操作进行，减少人为测量误差或问题，并允许通用自动分析仪器和多通道测量与其他测试项目连续测量。

公开(公告)号：US07148063B2

公开(公告)日：2006-12-12

申请号：US10424740

申请日：2003-04-29

申请人： Yasushi Shirahase ,  Tadahiro Kajita ,  Koji Kishi ,  Kazuaki Yamashita

发明人： Yasushi Shirahase ,  Tadahiro Kajita ,  Koji Kishi ,  Kazuaki Yamashita

IPC分类号： C12N5/20 ,  C07K16/40 ,  G01N33/53

CPC分类号： C07K16/40 ,  Y10S435/975

摘要： The object of the present invention is to provide a method for more accurately assaying the enzyme activities of mCK isozymes and a method for separately assaying the enzyme activities of CK isozymes by separately assaying the enzyme activities of ubiquitous mCK (umCK) and sarcomeric mCK (smCK). The above object is attained by an assay using an antibody that specifically recognizes umCK protein. In addition, other anti-mCK antibodies (e.g., anti-smCK antibody) and/or anti-human CK-M-inhibiting antibody can also be used. The above antibody is a polyclonal antibody or a monoclonal antibody. As a result of these antibodies, a monoclonal antibody (U1-1881) that is capable of specifically recognizing human umCK and is produced by a hybridoma having a deposition number of FERM BP-8342 is provided.

公开(公告)号：US07074581B2

公开(公告)日：2006-07-11

申请号：US10633518

申请日：2003-08-05

申请人： Kazuaki Yamashita ,  Yasushi Shirahase

发明人： Kazuaki Yamashita ,  Yasushi Shirahase

IPC分类号： C12Q1/60

CPC分类号： G01N33/92 ,  C12Q1/44 ,  C12Q1/60

摘要： Effective stabilizing amount at least of one antioxidant is added to a composition containing an esterase and surfactant(s).

摘要翻译： 将至少一种抗氧化剂的有效稳定化量加入到含有酯酶和表面活性剂的组合物中。

公开(公告)号：US06586194B1

公开(公告)日：2003-07-01

申请号：US09665063

申请日：2000-09-19

申请人： Yasushi Shirahase ,  Tadahiro Kajita ,  Tadashi Hoshino

发明人： Yasushi Shirahase ,  Tadahiro Kajita ,  Tadashi Hoshino

IPC分类号： G01N3300

CPC分类号： G01N33/573 ,  C12Q1/50 ,  Y10S435/81

摘要： A method for measuring the activity of creatine kinase MB (CK-MB) isozyme which is accurate, high in specificity, convenient, by inhibiting the activity of a mitochondria localized creatine kinase (mCK) isozyme to avoid the influence of mCK and a measurement reagent therefor.

摘要翻译： 通过抑制线粒体局部肌酸激酶（mCK）同功酶的活性来测定肌酸激酶MB（CK-MB）同功酶的活性，该方法准确，特异性高，方便，以避免mCK和测定试剂的影响 因此。

公开(公告)号：US5250416A

公开(公告)日：1993-10-05

申请号：US823113

申请日：1992-01-21

申请人： Tsuyoshi Ohno ,  Masaru Suzuki ,  Tatsuo Horiuchi ,  Yasushi Shirahase ,  Koji Kishi ,  Yoshifumi Watazu

发明人： Tsuyoshi Ohno ,  Masaru Suzuki ,  Tatsuo Horiuchi ,  Yasushi Shirahase ,  Koji Kishi ,  Yoshifumi Watazu

IPC分类号： C12Q1/26 ,  C12Q1/00 ,  C12Q1/32 ,  C12Q1/48 ,  C12N9/96 ,  C12P19/36

CPC分类号： C12Q1/48 ,  C12Q1/008

摘要： The present invention relates to a method for determining a slight amount of NADH or XTP which is present in a solution, with high sensitivity by the use of an NADH kinase specific for NADH by utilizing a cycling reaction, and this method permits highly sensitive determination of NADH without any influence of NAD.sup.+ and hence is useful for diagnoses of diseases and the like.

公开(公告)号：US5122455A

公开(公告)日：1992-06-16

申请号：US483747

申请日：1990-02-23

申请人： Yasushi Shirahase ,  Yoshifumi Watazu

发明人： Yasushi Shirahase ,  Yoshifumi Watazu

IPC分类号： C12Q1/37 ,  C12Q1/52

CPC分类号： C12Q1/52 ,  C12Q1/37

摘要： A glutamic-oxaloacetic transaminase (GOT) isozyme assay, which comprises selectively inhibiting s-GOT in a sample such as serum or plasma by proteinase K, optionally an excess amount of proteinase K by a protease inhibitor and then determining m-GOT remaining intact, is described.