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20 条记录 (耗时 0.026 秒)

    NADH kinase and a process for producing the same
    2.
    发明授权
    NADH kinase and a process for producing the same 失效
    NADH激酶及其生产方法

    公开(公告)号：US5227299A

    公开(公告)日：1993-07-13

    申请号：US822906

    申请日：1992-01-21

    申请人： Tsuyoshi Ohno ,  Masaru Suzuki ,  Tatsuo Horiuchi

    发明人： Tsuyoshi Ohno ,  Masaru Suzuki ,  Tatsuo Horiuchi

    IPC分类号： C12N9/12 ,  C12Q1/48 ,  C12R1/84

    CPC分类号： C12N9/12 ,  Y10S435/938

    摘要： The present invention relates to a novel NADH kinase which has high stability and is specific for NADH, and a process for producing the NADH kinase by culturing a yeast belonging to the genus Pichia in a culture medium, and this enzyme permits highly sensitive determination of NADH alone and hence is useful in the field of clinical medicine.

    摘要翻译： 本发明涉及一种具有高稳定性且对NADH具有特异性的新型NADH激酶，以及通过在培养基中培养属于毕赤酵母属的酵母来生产NADH激酶的方法，并且该酶能够高度敏感地测定NADH 因此在临床医学领域是有用的。

    Cycloisomaltooligosaccharides
    3.
    发明授权
    Cycloisomaltooligosaccharides 失效
    环状异麦芽低聚糖

    公开(公告)号：US5364936A

    公开(公告)日：1994-11-15

    申请号：US174596

    申请日：1993-12-28

    申请人： Tetsuya Oguma ,  Tatsuo Horiuchi ,  Koichiro Tobe

    发明人： Tetsuya Oguma ,  Tatsuo Horiuchi ,  Koichiro Tobe

    IPC分类号： C08B37/02 ,  C12N9/44 ,  C12P19/18 ,  C08B37/16

    CPC分类号： C12N9/2451 ,  C08B37/0021 ,  C12P19/18

    摘要： The present invention relates to a novel cycloisomaltooligosaccharide selected from the group consisting of novel cycloisomaltoheptaose having a cyclic structure composed of 7 glucose residues in .alpha.-1,6 linkage, novel cycloisomaltooctaose having a cyclic structure composed of 8 glucose residues in .alpha.-1,6 linkage and novel cycloisomaltononaose having a cyclic structure composed of 9 glucose residues in .alpha.-1,6 linkage, novel cycloisomaltooligosaccharide synthase forming said oligosaccharides from dextran, and a process for producing said oligosaccharides by use of said enzyme or a microorganism capable of producing said enzyme.

    摘要翻译： 本发明涉及一种选自下组的新型环状半缩水甘露寡糖，其由具有由α-1,6键连接的7个葡萄糖残基组成的环状结构的新型环异麦芽糖糖，具有由α-1,6中的8个葡萄糖残基组成的环状结构的新型环状体细菌 连接和具有由α-1,6键连接的9个葡萄糖残基组成的环状结构的新型环状马耳骨霉素，以及由右旋糖酐形成所述寡糖的新型环状二缩水甘露糖合成酶，以及通过使用所述酶或能够产生所述酶的微生物来生产所述寡糖的方法 。

    Method for assay of .alpha.-amylase activity
    4.
    发明授权
    Method for assay of .alpha.-amylase activity 失效
    α-淀粉酶活性测定方法

    公开(公告)号：US4395487A

    公开(公告)日：1983-07-26

    申请号：US256194

    申请日：1981-04-21

    申请人： Narimasa Saito ,  Tatsuo Horiuchi

    发明人： Narimasa Saito ,  Tatsuo Horiuchi

    IPC分类号： C12Q1/40

    CPC分类号： C12Q1/40

    摘要： A method for the assay of .alpha.-amylase activity, which comprises adding an .alpha.-amylase-containing sample to maltohexaitol or maltohexaonic acid used as substrate, reacting, at the same time or subsequent to the addition, .alpha.-glucosidase with the resulting mixture, and determining the reaction product to assay the .alpha.-amylase activity.

    摘要翻译： 一种测定α-淀粉酶活性的方法，其包括将含有α-淀粉酶的样品添加到用作底物的麦芽六糖醇或麦芽六糖酸中，同时或之后加入α-葡糖苷酶与所得混合物， 并测定反应产物以测定α-淀粉酶活性。

    L-fucose dehydrogenase, a process for production thereof, quantitative assay for L-fucose using said enzyme and a kit for quantitative assay
    5.
    发明授权
    L-fucose dehydrogenase, a process for production thereof, quantitative assay for L-fucose using said enzyme and a kit for quantitative assay 失效
    L-岩藻糖脱氢酶，其生产方法，使用所述酶定量测定L-岩藻糖和用于定量测定的试剂盒

    公开(公告)号：US5260197A

    公开(公告)日：1993-11-09

    申请号：US407148

    申请日：1989-09-14

    申请人： Tatsuo Horiuchi ,  Minoru Hiruma ,  Toshiyuki Suzuki

    发明人： Tatsuo Horiuchi ,  Minoru Hiruma ,  Toshiyuki Suzuki

    IPC分类号： C12N9/04 ,  C12Q1/26 ,  C12Q1/32 ,  C12R1/38

    CPC分类号： C12N9/0006 ,  C12Q1/32

    摘要： L-Fucose dehydrogenase having the following Physicochemical properties (1) and (2):(1) Action and substrate specificityIt withdraws hydrogen from L-fucose and converts into L-fuconolactone and at the same time, reduces coenzyme NADP.sup.+ to NADPH.(2) pH and stable pH rangeWhen Tris-imidazole-sodium acetate buffer solution is used, the optimum pH is in a range of 9.0 to 10.0 and the stable pH range is between 8.0 and 10.5.The L-fucose dehydrogenase utilizing NADP.sup.+ as coenzyme can be obtained by culturing L-fucose dehydrogenase-producing strain belonging to the genus Pseudomonas in a medium and collecting formed L-fucose dehydrogenase from the culture.L-Fucose can be quantitatively determined by reacting a sample containing L-fucose with L-fucose dehydrogenase requiring NADP.sup.+ as coenzyme and assaying NADPH produced.

    摘要翻译： 具有以下物理化学性质（1）和（2）的L-岩藻糖脱氢酶：（1）作用和底物特异性从L-岩藻糖中提取氢并转化为L- uconolactone，同时将辅酶NADP +还原成NADPH。 （2）pH和稳定的pH范围当使用三咪唑 - 乙酸钠缓冲溶液时，最佳pH在9.0至10.0的范围内，稳定的pH范围在8.0和10.5之间。 利用NADP +作为辅酶的L-岩藻糖脱氢酶可以通过在培养基中培养属于假单胞菌属的L-岩藻糖脱氢酶生产菌株并从培养物收集形成的L-岩藻糖脱氢酶而获得。 可以通过使含有L-岩藻糖的样品与需要NADP +的L-岩藻糖脱氢酶作为辅酶并测定产生的NADPH来定量测定L-岩藻糖。

    N-acetylhexosamine oxidase and process for producing the same
    6.
    发明授权
    N-acetylhexosamine oxidase and process for producing the same 失效
    N-乙酰己糖胺氧化酶及其制造方法

    公开(公告)号：US4621057A

    公开(公告)日：1986-11-04

    申请号：US580243

    申请日：1984-02-15

    申请人： Tatsuo Horiuchi

    发明人： Tatsuo Horiuchi

    IPC分类号： C12N9/06 ,  C12N9/04 ,  C12R1/38

    CPC分类号： C12N9/0006 ,  Y10S435/874

    摘要： An N-acetylhexosamine oxidase having the following physiochemical properties.(1) Action and specificity for substrateOxidizes N-acetylhexosamine in the presence of oxygen to form N-acetylhexosaminic acid and hydrogen peroxide. Hardly acts or does not act at all on hexose and hexosamine.(2) Optimum pH and stable pH rangeWhen a potassium phosphate buffer solution contining 0.1M glycine is used, an optimum pH is 7.5 to 8.5 and a stable pH range is 3 to 9.(3) Molecular weightHas a molecular weight of about 140,000 to 150,000 when measured according to a gel filtration method using Sephadex G-200 by the use of 0.05M potassium phosphate buffer solution.

    摘要翻译： 具有以下理化特性的N-乙酰基己糖胺氧化酶。 （1）底物的作用和特异性在氧气存在下氧化N-乙酰基己糖胺以形成N-乙酰基己糖胺酸和过氧化氢。 在己糖和己糖胺上几乎不起作用或不起作用。 （2）最佳pH值和稳定的pH范围当使用连续使用0.1M甘氨酸的磷酸钾缓冲溶液时，最适pH为7.5〜8.5，pH范围为3〜9。（3）分子量约为 根据使用Sephadex G-200通过使用0.05M磷酸钾缓冲溶液的凝胶过滤方法测量时，为140,000至150,000。

    Auxiliary head lamp device for car equipped with cover
    10.
    发明授权
    Auxiliary head lamp device for car equipped with cover 失效
    汽车辅助前灯装置配有盖

    公开(公告)号：US4443836A

    公开(公告)日：1984-04-17

    申请号：US363514

    申请日：1982-03-30

    申请人： Tatsuo Horiuchi ,  Kazushige Kiyota

    发明人： Tatsuo Horiuchi ,  Kazushige Kiyota

    IPC分类号： B60Q1/05 ,  B60Q1/18 ,  F21V15/00 ,  F21V17/00

    CPC分类号： B60Q1/18 ,  B60Q1/05

    摘要： The invention discloses an auxiliary head lamp device for a car equipped with a cover which is optionally fitted to a car and is lit during driving in fog or the like. The cover of this device can be opened when a solenoid of a cover driving mechanism is energized by operating a switch disposed inside the room of the car. The solenoid releases engagement of a stopper that restricts the cover urged in the opening direction. The switch may be used also as a switch for a light source bulb. After the solenoid is energized and the opening operation of the cover starts, a current to the solenoid is automatically cut off so that over-heat or burn-out of the solenoid that would occur if the current is continuously applied thereto can be prevented.

    摘要翻译： 本发明公开了一种用于车辆的辅助头灯装置，其配备有可选地安装在汽车上的盖子，并且在雾中驾驶期间点亮等。 当通过操作设置在汽车的房间内的开关来给盖驱动机构的螺线管通电时，可以打开该装置的盖。 螺线管释放限制沿打开方向推动的盖的止动器的接合。 该开关也可以用作光源灯泡的开关。 在螺线管通电并且盖子的打开操作开始之后，自动切断到螺线管的电流，从而可以防止如果电流被连续地施加到电磁阀时会发生的螺线管的过热或烧坏。