公开(公告)号：US5137807A

公开(公告)日：1992-08-11

申请号：US380751

申请日：1989-07-17

申请人： Shinichi Yoshida ,  Eiji Ishikawa ,  Akira Oshima ,  Yasuo Bai ,  Yasuteru Muragaki ,  Kazushi Iwata ,  Kenichi Obata ,  Yasuo Nagai

发明人： Shinichi Yoshida ,  Eiji Ishikawa ,  Akira Oshima ,  Yasuo Bai ,  Yasuteru Muragaki ,  Kazushi Iwata ,  Kenichi Obata ,  Yasuo Nagai

IPC分类号： A61K39/00 ,  C12Q1/00 ,  C12Q1/26 ,  G01N33/573 ,  G01N33/577

CPC分类号： G01N33/573 ,  Y10S436/811 ,  Y10S436/82

摘要： The present invention is directed to a method for detecting hepatic diseases which are associated with fibrosis by determining the level of human prolyl hydroxylase in a serum sample which comprises:(a) contacting a serum sample of a patient suspected of having said hepatic disease associated with fibrosis with a monoclonal antibody specific to the .beta.-subunit of human prolyl hydroxylase to form an antigen antibody complex bound on a solid support;(b) contacting said antigen antibody complex bound on said solid support with an enzyme-labeled monoclonal or enzyme-labeled polyclonal antibody specific to human prolyl hydroxylase to form an antibody antigen enzyme-labeled antibody complex; and(c) measuring the amount of enzyme activity of said bound antibody antigen enzyme-labeled antibody complex to determine the level of human prolyl hydroxylase present in said serum sample.

摘要翻译： 本发明涉及通过测定血清样品中人脯氨酰羟化酶水平来检测与纤维化相关的肝病的方法，该方法包括：（a）使疑似患有所述肝病的患者的血清样品与 纤维化与对人脯氨酰羟化酶的β亚单位特异的单克隆抗体形成结合在固体支持物上的抗原抗体复合物; （b）将结合在所述固体支持物上的所述抗原抗体复合物与人脯氨酰羟化酶特异性的酶标记的单克隆或酶标记的多克隆抗体接触以形成抗体抗原酶标记的抗体复合物; 和（c）测量所述结合的抗体抗体酶标记的抗体复合物的酶活性的量以确定存在于所述血清样品中的人脯氨酰羟化酶的水平。

公开(公告)号：US5316914A

公开(公告)日：1994-05-31

申请号：US831645

申请日：1992-02-07

申请人： Akira Oshima ,  Kazushi Iwata ,  Yasuteru Muragaki ,  Yasuo Bai ,  Eisaku Matsumoto ,  Satoshi Miyamoto

发明人： Akira Oshima ,  Kazushi Iwata ,  Yasuteru Muragaki ,  Yasuo Bai ,  Eisaku Matsumoto ,  Satoshi Miyamoto

IPC分类号： G01N33/68 ,  G01N33/543 ,  G01N33/576 ,  G01N33/577

CPC分类号： G01N33/6887

摘要： A method for determining human type III, IV and VI collagen peptides by way of enzyme immunoassay according to the sandwich technique using a monoclonal antibody and a polyclonal antibody each to the human type III, IV or VI collagen peptide, characterized in that the monoclonal antibody to the collagen peptide is used as an antibody to be coated onto a solid phase and/or an antibody to be labeled with an enzyme. Also disclosed is a sandwich technique using two different monoclonal antibodies. This method can be carried out with a smaller amount of samples in a simple manner to obtain a precise result and is thus useful for diagnosis of hepatic diseases.

摘要翻译： 一种通过使用单克隆抗体和针对人III，IV或VI型胶原肽的多克隆抗体的夹心技术通过酶免疫测定法测定人III，IV和VI型胶原肽的方法，其特征在于单克隆抗体 使用胶原肽作为要涂覆在待用酶标记的固相和/或抗体上的抗体。 还公开了使用两种不同单克隆抗体的夹心技术。 该方法可以用较少量的样品以简单的方式进行，以获得精确的结果，因此可用于诊断肝脏疾病。