公开(公告)号：US08394850B2

公开(公告)日：2013-03-12

申请号：US12920684

申请日：2009-03-04

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Masahiro Abo

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Masahiro Abo

IPC分类号： A61K31/352 ,  C07D311/82

CPC分类号： C07D311/82 ,  G01N31/22 ,  G01N2021/7786 ,  Y10T436/206664

摘要： A compound represented by the following general formula (IA) or (IB) (R1 represents an electron withdrawing substituent, R2 and R3 represent a hydrogen atom or a halogen atom; R4 and R3 represent a hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, and R6 represents a hydrogen atom or an alkyl group) or a salt thereof, and a reagent for measuring hydrogen peroxide comprising the compound or a salt thereof.

摘要翻译： 由以下通式（IA）或（IB）表示的化合物（R1表示吸电子取代基，R2和R3表示氢原子或卤素原子; R4和R3表示氢原子，烷基羰基或烷基羰氧基甲基 基团，R6表示氢原子或烷基）或其盐，以及含有该化合物或其盐的测量过氧化氢的试剂。

公开(公告)号：US08258171B2

公开(公告)日：2012-09-04

申请号：US12447723

申请日：2007-11-15

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Daisuke Asanuma

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Daisuke Asanuma

IPC分类号： A61K31/40 ,  C07D209/56

CPC分类号： C07F5/022 ,  G01N21/80

摘要： A compound represented by the following general formula (I): wherein R1 represents an amino group which may be substituted with one or two alkyl groups; R2, R3, R4 and R5 independently represent an alkyl group; and R6 and R7 independently represent a monocarboxyalkyl group, a salt thereof, or an ester thereof, which is useful as a pH-sensitive fluorescent probe which emits intense fluorescence in an acidic region.

摘要翻译： 由以下通式（I）表示的化合物：其中R1表示可被一个或两个烷基取代的氨基; R2，R3，R4和R5独立地表示烷基; 并且R6和R7独立地表示单羧基烷基，其盐或其酯，其可用作在酸性区域发射强荧光的pH敏感荧光探针。

公开(公告)号：US20110251404A1

公开(公告)日：2011-10-13

申请号：US12991750

申请日：2009-05-15

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

IPC分类号： C07D311/82 ,  C07D311/90

CPC分类号： G01N31/225 ,  C07D311/82 ,  C07D493/10 ,  G01N2021/7786

摘要： A compound represented by the formula (I) (R1 represents an aryl group such as hydroxyphenyl group, R2 represents 2-carboxyphenyl group etc., and R3 and R4 represent —(CH2)p—N(R5)(R6) (R5 and R6 represent —(CH2)n—COOH), a salt thereof, or an ester thereof, which is useful as a reagent for highly sensitive measurement of reactive oxygen such as hydroxy radical present at a trace amount in living bodies or tissues by a bioimaging technique over a long period of time.

摘要翻译： 由式（I）表示的化合物（R 1表示芳基如羟基苯基，R 2表示2-羧基苯基等，R 3和R 4表示 - （CH 2）p -N（R 5）（R 6）（R 5和 R6表示 - （CH2）n-COOH），其盐或其酯，可用作活性氧如活体或组织中微量存在的活性氧的高灵敏度测定的试剂，通过生物成像 技术在很长一段时间内。

公开(公告)号：US20110033944A1

公开(公告)日：2011-02-10

申请号：US12910457

申请日：2010-10-22

申请人： Ken-ichi SETSUKINAI ,  Yasuteru URANO ,  Tetsuo NAGANO

发明人： Ken-ichi SETSUKINAI ,  Yasuteru URANO ,  Tetsuo NAGANO

IPC分类号： G01N21/76

CPC分类号： G01N21/6428 ,  G01N2021/7786 ,  Y10T436/14 ,  Y10T436/19 ,  Y10T436/196666 ,  Y10T436/200833

摘要： A method for measuring hypochlorite ion, which comprises the steps of:(A) reacting, with hypochlorite ion, a compound represented by the following general formula (I): wherein R1 represents a 2-carboxyphenyl group which may be substituted; R2 represents a phenyl group which is substituted with a substituted or unsubstituted amino group; X1 and X2 each independently represents either hydrogen atom or a halogen atom; or a salt thereof; and (B) measuring fluorescence of a dearylated compound generated in the aforementioned step (A) or a salt thereof.

摘要翻译： 一种测定次氯酸根离子的方法，包括以下步骤：（A）与次氯酸根离子反应由以下通式（I）表示的化合物：其中R1表示可被取代的2-羧基苯基; R2表示被取代或未取代的氨基取代的苯基; X1和X2各自独立地表示氢原子或卤素原子; 或其盐; 和（B）测量在上述步骤（A）中产生的脱芳基化合物的荧光或其盐。

公开(公告)号：US20090318707A1

公开(公告)日：2009-12-24

申请号：US12066323

申请日：2006-09-13

申请人： Takuya Matsumoto ,  Takuji Shoda ,  Yasuteru Urano ,  Tetsuo Nagano

发明人： Takuya Matsumoto ,  Takuji Shoda ,  Yasuteru Urano ,  Tetsuo Nagano

IPC分类号： C07F5/02

CPC分类号： C07F5/022 ,  C09B23/04 ,  C09B57/10 ,  G01N21/6428 ,  G01N2021/6439

摘要： A compound represented by the following general formula (I): [wherein R1 and R2 independently represent hydrogen atom, or a group represented by the following formula (A): (wherein X1 and X2 represent hydrogen atom, an alkyl group, an alkoxy group, or a halogen atom), provided that R1 and R2 do not simultaneously represent hydrogen atom; R3 and R6 represent an alkyl group; R4 and R7 represent hydrogen atom, an alkyl group, carboxy group, an alkoxycarbonyl group, or sulfo group; and R5 and R8 represents an alkyl group, an aryl group, an alkoxycarbonyl group, a vinyl group, a thienyl group, or a pyrrolyl group], or a salt thereof, which is usable for efficient screening for a chemical substance having applicability as a catalyst of the reaction of Michael addition.

摘要翻译： 由以下通式（I）表示的化合物：[其中R 1和R 2独立地表示氢原子或由下式（A）表示的基团：（其中X 1和X 2表示氢原子，烷基，烷氧基 或卤素原子），条件是R 1和R 2不同时表示氢原子; R3和R6表示烷基; R4和R7表示氢原子，烷基，羧基，烷氧基羰基或磺基; R5和R8表示烷基，芳基，烷氧基羰基，乙烯基，噻吩基或吡咯基]或其盐，其可用于有效筛选具有适用性的化学物质作为 催化剂的迈克尔加成反应。

公开(公告)号：US07524876B2

公开(公告)日：2009-04-28

申请号：US11493815

申请日：2006-07-27

申请人： Hideo Takakura ,  Yasuteru Urano ,  Tetsuo Nagano

发明人： Hideo Takakura ,  Yasuteru Urano ,  Tetsuo Nagano

IPC分类号： A61K31/428 ,  C07D277/62 ,  C07D417/04

CPC分类号： C07D277/68

摘要： A compound represented by the following general formula (I) or a salt thereof: [wherein R1 and R2 represent hydrogen atom, a C1-6 alkyl group, or a group represented by the following formula (A): [wherein X1 and X2 represent hydrogen atom, or a group represented as —N(R3)(R4) (R3 and R4 represent hydrogen atom, a C1-6 alkyl group, a C1-6 alkylcarbonyl group, or a C1-6 alkyloxycarbonyl group); and n represents an integer of 1 to 6 ], provided that R1 and R2 do not simultaneously represent hydrogen atom), which is a novel luciferin derivative that serves as a luciferase substrate.

摘要翻译： 由以下通式（I）表示的化合物或其盐：[其中R1和R2表示氢原子，C1-6烷基或由下式（A）表示的基团：[其中X1和X2表示 或者表示为-N（R3）（R4）的基团（R3和R4表示氢原子，C1-6烷基，C1-6烷基羰基或C1-6烷氧基羰基）表示的基团。 并且n表示1〜6的整数]，条件是R1和R2不同时表示氢原子），其是用作荧光素酶底物的新型荧光素衍生物。

公开(公告)号：US20080014602A1

公开(公告)日：2008-01-17

申请号：US10570355

申请日：2004-09-03

申请人： Tetsuo Nagano ,  Mako Kamiya ,  Yasuteru Urano

发明人： Tetsuo Nagano ,  Mako Kamiya ,  Yasuteru Urano

IPC分类号： C12Q1/34 ,  G01N21/64 ,  G01N21/78

CPC分类号： C09B11/08 ,  C12Q1/34

摘要： A fluorescent probe which is represented by the following formula (I): (wherein, R1 represents a monovalent substituent other than hydrogen atom, carboxy group, or sulfo group; R2 represents hydrogen atom, or a monovalent substituent; R3 and R4 each independently represents hydrogen atom or a halogen atom; and R5 represents a monovalent group which is cleaved by contact with a measuring object, provided that a combination of R1 and R2 is selected so that the oxidation potential of the benzene ring to which they bind makes (1) the compound represented by the formula (I) substantially no fluorescent before the cleavage, and (2) a compound after the cleavage, which is derived from the compound represented by the formula (I), substantially highly fluorescent after the cleavage).

摘要翻译： 由下式（I）表示的荧光探针：（其中，R 1表示除氢原子，羧基或磺基以外的一价取代基; R 2 O >表示氢原子或一价取代基; R 3和R 4各自独立地表示氢原子或卤素原子; R 5表示 如果选择R 1和R 2的组合，使得与测量对象接触而被切割的一价基团，使得苯环的氧化电位 它们的结合使（1）式（I）表示的化合物在切割前基本上不发荧光，和（2）衍生自由式（I）表示的化合物的裂解后的化合物在 裂解）。

公开(公告)号：US20070155806A1

公开(公告)日：2007-07-05

申请号：US11493815

申请日：2006-07-27

申请人： Hideo Takakura ,  Yasuteru Urano ,  Tetsuo Nagano

发明人： Hideo Takakura ,  Yasuteru Urano ,  Tetsuo Nagano

IPC分类号： A61K31/428 ,  C07D417/04

CPC分类号： C07D277/68

摘要： A compound represented by the following general formula (I) or a salt thereof: [wherein R1 and R2 represent hydrogen atom, a C1-6 alkyl group, or a group represented by the following formula (A): [wherein X1 and X2 represent hydrogen atom, or a group represented as —N(R3)(R4) (R3 and R4 represent hydrogen atom, a C1-6 alkyl group, a C1-6 alkylcarbonyl group, or a C1-6 alkyloxycarbonyl group); and n represents an integer of 1 to 6], provided that R1 and R2 do not simultaneously represent hydrogen atom), which is a novel luciferin derivative that serves as a luciferase substrate.

摘要翻译： 由下列通式（I）表示的化合物或其盐：其中R 1和R 2代表氢原子，C 1-6烷基， 或由下式（A）表示的基团：[其中X 1和X 2表示氢原子或表示为-N （R 3）（R 4）（R 3和R 4）代表氢原子，C

公开(公告)号：US20070074967A1

公开(公告)日：2007-04-05

申请号：US11366464

申请日：2006-03-03

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Tomonori Kobayashi ,  Noriyuki Suzuki

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Tomonori Kobayashi ,  Noriyuki Suzuki

IPC分类号： C07C209/68 ,  C07C39/04 ,  C07C209/00

CPC分类号： C07D311/82 ,  Y02P20/55

摘要： A photodegradable protective group comprising a benzyl group which is bound with a light capturing moiety that can absorb light of around 470 nm and having one or more nitro groups on the benzene ring.

摘要翻译： 包含与可吸收约470nm的光并且在苯环上具有一个或多个硝基的光捕获部分结合的苄基的光可降解保护基。

公开(公告)号：US20060275912A1

公开(公告)日：2006-12-07

申请号：US10547305

申请日：2004-02-27

申请人： Tetsuo Nagano ,  Yu Gabe ,  Yasuteru Urano

发明人： Tetsuo Nagano ,  Yu Gabe ,  Yasuteru Urano

IPC分类号： C07F5/02 ,  G01N33/00

CPC分类号： C07F5/022 ,  C09K11/06 ,  C09K2211/1055 ,  G01N31/22 ,  Y10T436/145555 ,  Y10T436/177692

摘要： A compound represented by the formula (I) wherein R1 and R2 represent amino groups that substitute at adjacent positions on the benzene ring, wherein one of the amino groups may have one alkyl group; R3 and R4 represent hydrogen atom, a C1-6 alkyl group, or a C1-6 alkoxy group, R5 and R8 independently represent a C1-6 alkyl group, R6 and R9 represent a carboxy-substituted C1-6 alkyl group, an alkoxycarbonyl-substituted C1-6 alkyl group, a sulfo-substituted C1-6 alkyl group, or an alkyl sulfonate-substituted C1-6 alkyl group, and R7 and R10 represent a C1-6 alkyl group, an aryl group, a C1-6 alkoxycarbonyl group, a vinyl group, a thienyl group, or a pyrrolyl group, and an agent for measurement of nitrogen monoxide containing the compound. Said compound can give a fluorescent substance that is free from change in fluorescence intensity in a wide pH range.

摘要翻译： 由式（I）表示的化合物，其中R 1和R 2代表在苯环上的相邻位置取代的氨基，其中氨基之一可具有 一个烷基; R 3和R 4代表氢原子，C 1-6烷基或C 1-6 - 烷基 >烷氧基，R 5和R 8独立地表示C 1-6烷基，R 6和 R 9表示羧基取代的C 1-6烷基，烷氧基羰基取代的C 1-6烷基，磺基取代的 C 1-6烷基或烷基磺酸酯取代的C 1-6烷基，R 7和R 10 代表C 1-6烷基，芳基，C 1-6烷氧基羰基，乙烯基，噻吩基或吡咯基 组和用于测量含有该化合物的一氧化氮的试剂。 所述化合物可以产生在宽pH范围内没有荧光强度变化的荧光物质。